A5032389760- Pancreatic and Hepatic Oncology Research
- Intraperitoneal and Appendiceal Malignancies
- Pancreatitis Pathology and Treatment
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Mast cells and histamine
- Receptor Mechanisms and Signaling
- Gastrointestinal motility and disorders
- 3D Printing in Biomedical Research
- Ion Transport and Channel Regulation
- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Urticaria and Related Conditions
- Ion Channels and Receptors
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Drug Transport and Resistance Mechanisms
- Asthma and respiratory diseases
- Diabetes Treatment and Management
- Clinical Nutrition and Gastroenterology
- Long-Term Effects of COVID-19
- Galectins and Cancer Biology
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Wnt/β-catenin signaling in development and cancer
Center for Cancer Research
2019-2024
National Cancer Institute
2018-2024
National Institutes of Health
2018-2024
Johns Hopkins Medicine
2015-2019
Johns Hopkins University
2015-2019
University of Antwerp
2009-2014
As COVID-19 continues, an increasing number of patients develop long COVID symptoms varying in severity that last for weeks, months, or longer. Symptoms commonly include lingering loss smell and taste, hearing loss, extreme fatigue, "brain fog." Still, persistent cardiovascular respiratory problems, muscle weakness, neurologic issues have also been documented. A major problem is the lack clear guidelines diagnosing COVID. Although some studies suggest due to prolonged inflammation after...
Background & AimsHuman enteroids present a novel tool to study human intestinal ion transport physiology and pathophysiology. The describes the contributions of Cl- HCO3- secretion total cyclic adenosine monophosphate (cAMP)-stimulated electrogenic anion in duodenal enteroid monolayers relevant changes after differentiation.MethodsHuman derived from 4 donors were grown as differentiated by protocol that includes removal Wnt3A, R-spondin1, SB202190 for 5 days. messenger RNA level protein...
Abstract Over 90% of pancreatic ductal adenocarcinomas (PDAC) express mesothelin (MSLN). Overexpression or knockdown MSLN has been implicated in PDAC aggressiveness. This activity ascribed to MSLN-induced activation MAPK NF-κB signaling pathways and interaction with its only known binding partner, MUC16. Here, we used CRISPR/Cas9 gene editing delete from PDAC, then restored expression wild-type (WT) Y318A mutant by viral transduction. We found that KO cells grew culture as subcutaneous...
Sorting nexin 27 (SNX27) contains a PDZ domain that is phylogenetically related to the domains of NHERF proteins. Studies on nonepithelial cells have shown this protein located in endosomes, where it regulates trafficking cargo proteins domain-dependent manner. However, role SNX27 epithelial has not been adequately explored. Here we show directly interacts with NHE3 (C-terminus) primarily through domain. A combination knockdown and reconstitution experiments wild type mutant (GYGF → GAGA)...
The expression of transient receptor potential vanilloid type 1 channel (TRPV1) in the enteric nervous system is still subject debate. Although a number studies have reported that TRPV1 limited to extrinsic afferent fibers, other argue for an intrinsic TRPV1. In present study, reverse transcriptase PCR was employed establish mRNA throughout gastrointestinal tract. Using two antibodies directed against different epitopes TRPV1, we were able show at protein level observed distribution pattern...
Despite significant improvements in methodologies behind the development of new anticancer therapies, path from early-stage drug through preclinical and clinical pipeline is still arduous. Further refinement current models complementing alternative techniques that enable more reliable studies are paramount. The emerging organ-on-a-chip (OoC) technologies physiological-like organ biomimetic systems built on a microfluidic chip, capable enabling precise control over various physicochemical...
The intestinal epithelial brush border Na+/H+ exchanger NHE3 accounts for a large component of Na absorption. is regulated during digestion by signaling complexes on its COOH terminus that include the four multi-PDZ domain-containing NHERF family proteins. All bind to and take part in different aspects regulation. Because roles each appear vary basis cell model or segment studied because our recent finding NHERF3-NHERF2 heterodimer appears important regulation Caco-2 cells, we examined role...
Abstract Background Although a number of intestinal inflammatory conditions pertain to the ileum, whole-genome gene expression analyses in animal models ileal inflammation are lacking date. Therefore, we aimed identify and characterize alterations acutely inflamed ileum two murine inflammation, namely schistosomiasis TNBS-induced ileitis, compared healthy controls. To this end, used microarrays, followed by bioinformatics detect over-represented Kyoto Encyclopedia Genes Genomes pathways Gene...
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of childhood death from diarrhea and the Traveler's diarrhea. E. heat-stable enterotoxin (ST) major virulence factor ETEC inhibits brush border Na/H exchanger NHE3 in producing regulation involves multiprotein signaling complexes that form on its COOH terminus. In this study, hypothesis was tested ST signals via members regulatory (NHERF) family scaffolding proteins, NHERF2, which had been previously shown to have role, now with...
Abstract Peritoneum is the second most common site of metastasis in patients with pancreatic ductal adenocarcinoma (PDAC). Peritoneal colonization impaired PDAC cells knockout (KO) cancer surface antigen mesothelin (MSLN) or by introducing Y318A mutation MSLN to prevent binding mucin‐16 (MUC‐16). has a membrane‐bound form but also shed release soluble (sMSLN). Their individual roles peritoneal are unknown. Here, C‐terminal truncated mutant (∆591) incapable cell membrane insertion proficient...
Some members of the Mas‐related gene receptor (Mrg) family have been suggested to play a role in mediating IgE‐independent mast cell activation, as well neuroimmune communication. In previous study, we observed an increased expression some Mrgs, such MrgD, murine ileum during intestinal inflammation. To further unravel distribution and involvement MrgD inflammation, compared non‐inflamed Schistosoma mansoni ‐infected wild‐type −/− mice. mice, ileum, immunohistochemistry revealed no...
Corticotropin releasing factor (CRF) and urocortins (UCNs) are important ligands in the CRF signaling pathways, most well‐known from hypothalamic‐pituitary‐adrenal stress axis. However, peripheral has profound effects on gastrointestinal functions. Despite potential of murine animal model elucidating this complexly balanced pathway through genetic manipulation, little is known regarding expression UCNs intestinal wall mouse. This study therefore investigates cellular localization ileum...
We have previously shown that the Mas‐related gene receptor D (MrgD) is expressed de novo in mouse intestine during inflammation, specifically intrinsic sensory neurons and mucosal mast cells (MMCs). also MrgD deletion leads to increased MMC recruitment neuropeptide expression. These findings indicate role(s) for regulation of mastocytosis (mast cell mediated)‐sensory neuromodulation. To further examine effect on intestinal function, we compared motility ileum healthy Schistosoma...
<p>In vivo BLI of mice inoculated IP with Luc/GFP+ KLM1 Mock or KO#2 on day 3 (D3), 7 (D7) and 14 (D14) post-injection.</p>
<p>Examination of involvement NF-κB pathway in MSLN IP pro-tumorigenicity.</p>
<p>Identifying a novel mediator for MSLN activity using qPCR.</p>