A5083176418- Folate and B Vitamins Research
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Aldose Reductase and Taurine
- Cannabis and Cannabinoid Research
- Amino Acid Enzymes and Metabolism
- Muscle metabolism and nutrition
- Endoplasmic Reticulum Stress and Disease
- HIV Research and Treatment
- Child Nutrition and Water Access
- Receptor Mechanisms and Signaling
- Dietary Effects on Health
- Sulfur Compounds in Biology
- Autophagy in Disease and Therapy
- Neuropeptides and Animal Physiology
- Neuroscience of respiration and sleep
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Diet and metabolism studies
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Gut microbiota and health
- Neurotransmitter Receptor Influence on Behavior
Orentreich Foundation for the Advancement of Science
2010-2025
SUNY Old Westbury
1998-1999
Stearoyl-CoA desaturase-1 (SCD1) is a key enzyme in fatty acid and energy metabolism, but little known about its nutritional regulation. Dietary methionine restriction rats decreases hepatic <i>Scd1</i> mRNA protein, increases expenditure, fat-pad mass/body-weight% (FM/BW%). In humans, plasma concentrations of the product, cysteine, are associated with obesity. To determine which consequences methionine-restriction mediated by decreased cysteine availability, we monitored obesity-related...
<b><i>Background/Aims:</i></b> Methionine restriction (MR) is a dietary intervention that increases lifespan, reduces adiposity and improves insulin sensitivity. These effects are reversed by supplementation of the MR diet with cysteine (MRC). Genomic metabolomic studies were conducted to identify potential mechanisms which induces favorable metabolic effects, supplementation. <b><i>Methods:</i></b> Gene expression was examined microarray...
Methionine restriction (MR) limits age-related adiposity in Fischer 344 (F344) rats. To assess the mechanism of resistance, effect MR on adipose tissue (AT) 11β-hydroxysteroid dehydrogenase-1 (11β-HSD1) was examined. induced 11β-HSD1 activity all ATs, correlating with increased corticosterone. However, an inverse relationship between and adipocyte size observed. Because dietary controls lipogenic lipolytic rates, MR's effects enzymes were evaluated. triglyceride lipase acetyl-coenzyme A...
Identifying novel approaches to combat obesity is important improve health span. It was hypothesized that methionine restriction (MR) will induce weight loss in obese mice by reducing adipose tissue mass caused increased energy expenditure and reprogramming of homeostasis. The roles adiponectin (ADIPOQ) fibroblast growth factor 21 (FGF21) during MR were also tested.
Sulfur amino acid restriction (SAAR), lowering the dietary concentration of sulfur acids methionine and cysteine, induces strong anti-obesity effects in rodents. Due to difficulties formulating SAAR diet for human consumption, its translation is challenging. Since our previous studies suggest a mechanistic role low glutathione (GSH) SAAR-induced effects, we investigated if pharmacological GSH recapitulates lean phenotype mice on acid-replete diet. Male obese C57BL6/NTac were fed high-fat...
Abstract The mechanisms underlying life span extension by sulfur amino acid restriction (SAAR) are unclear. Cysteine and methionine essential for the biosynthesis of proteins glutathione (GSH), a major redox buffer in endoplasmic reticulum (ER). We hypothesized that SAAR alters protein synthesis modulating milieu. Male F344‐rats were fed control (CD: 0.86% without cysteine) diets (0.17% 12 weeks. Growth rates, food intake, cysteine GSH levels, associated with status translation, fractional...
Decreasing the dietary intake of methionine exerts robust anti-adiposity effects in rodents but modest humans. Since cysteine can be synthesized from methionine, animal diets are formulated by decreasing and eliminating cysteine. Such exert both restriction (MR) (CR), that is, sulfur amino acid (SAAR). Contrarily, SAAR for human consumption included cysteine, thus might have exerted only MR. Epidemiological studies positively correlate body adiposity with plasma not suggesting CR, MR, is...
Abstract Protein folding in the endoplasmic reticulum (ER) requires a high ratio of oxidized to reduced glutathione (GSSG/rGSH). Since GSSG/rGSH depends on total (tGSH = GSSG + rGSH) levels, we hypothesized that limiting GSH biosynthesis will ameliorate protein misfolding by enhancing ER oxidative milieu. As proof-of-concept, used DL-buthionine-(S,R)-sulfoximine (BSO) inhibit Akita mice, which are prone proinsulin misfolding. We conducted 2-week intervention investigate if BSO was safe and...
Abstract Trade‐offs in life‐history traits are clinically and mechanistically important. Sulfur amino acid restriction (SAAR) extends lifespan. But whether this benefit comes at the cost of other including stress resistance growth is unclear. We investigated effects SAAR on markers (body weight, IGF1, IGFBP3) physiological stresses. Male‐F344 rats were fed control (0.86% Met) (0.17% diets starting 2, 10, 20 months. Rats injected with keyhole‐limpet‐hemocyanin (KLH) to measure immune...
Abstract Diet can affect health and longevity by altering the gut microbiome profile. Sulfur amino acid restriction (SAAR), like caloric restriction, extends lifespan. But, its effect on profile functional significance of such effects are understudied. We investigated whether SAAR alters bile composition, an index microbial metabolism. also compared these changes with those induced a 12% low-calorie diet (LCD). Male 21-week-old C57BL6/J mice were fed control (CD; 0.86% methionine), (0.12%...
Summary Decreasing dietary intake of methionine exerts robust anti-adiposity effects in rodents but modest humans. Since cysteine can be synthesized from methionine, animal diets are formulated by decreasing and eliminating cysteine. Such exert both restriction (MR) (CR), i.e., sulfur amino acid (SAAR). Contrarily, human used clinical studies did not eliminate thus might have exerted only MR. Epidemiological positively correlate body adiposity with plasma suggesting that CR, MR, is...