A5068823299- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- Enzyme function and inhibition
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Bone health and treatments
- Vitamin D Research Studies
- Renin-Angiotensin System Studies
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Disease and Adiposity
- Medical Imaging and Pathology Studies
- RNA modifications and cancer
- Neuroblastoma Research and Treatments
- ATP Synthase and ATPases Research
- melanin and skin pigmentation
- Metabolism and Genetic Disorders
- CRISPR and Genetic Engineering
- Chromatin Remodeling and Cancer
- Amino Acid Enzymes and Metabolism
- Bone and Joint Diseases
- Nuclear Structure and Function
- Cholinesterase and Neurodegenerative Diseases
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- RNA Research and Splicing
Ceinge Biotecnologie Avanzate (Italy)
2012-2025
University of Naples Federico II
2012-2025
Federico II University Hospital
2022
Associazione Italiana Vulvodinia Onlus
2018
Cellular senescence is a permanent cell cycle arrest triggered by different stimuli. We recently identified up-regulation of microRNA (miR)-494 as component the genetic program leading to human diploid IMR90 fibroblasts. Here, we used 2-dimensional differential gel electrophoresis (2D-DIGE) coupled mass spectrometry profile protein expression changes induced adoptive overexpression miR-494 in cells. robust perturbation proteome significantly (P<0.05) down-regulating number proteins....
Carbonic anhydrases constitute a family of metalloenzymes vital for maintaining acid-base balance and regulating pH in physio-pathological processes. These findings suggest carbonic as potential therapeutic targets treating pH-associated disorders, including cerebral ischemia, to mitigate hypoxia- reoxygenation-induced neuronal damage. A focus on anhydrase IX showed that ischemic stress altered subcellular distributions this enzyme rodent populations. Given the enzyme's canonical membrane...
Use of monoclonal antibodies is emerging as a highly promising and fast-developing scenario for innovative treatment viral, autoimmune tumour diseases. The search diagnostic therapeutic currently depends on in vitro screening approaches, such phage yeast display technologies. Antibody production still represents critical step preclinical clinical evaluations. Accordingly, improving an opportunity, to facilitate downstream target validations. SINEUP RNAs are long non-coding transcripts,...
Cetuximab is a chimeric antibody approved for the treatment of metastatic colorectal cancer that selectively targets epidermal growth factor receptor (EGFR) signaling. Treatment efficacy with this drug often impaired by acquired resistance and poor information has been accumulated on mechanisms underlying such phenomenon. By taking advantage syngenic cellular system sensitivity to anti-EGFR therapy in carcinoma GEO cell line, we profiled protein expression differences between...
In the last decades, cellular senescence is viewed as a complex mechanism involved in different processes, ranging from tumor suppression to induction of age-related degenerative alterations. Senescence-inducing stimuli are myriad and, recently, we and others have demonstrated role exerted by microRNAs maintenance senescence, identification subset Senescence-Associated (SAmiRs) up-regulated during replicative or stress-induced able induce premature senescent phenotype when over-expressed...
Human Carbonic Anhydrase (hCA) IX is a membrane-associated member of the CA enzyme family, involved in solid tumor acidification. This marker hypoxia and prognostic factor for several human cancers. In recent paper, we showed that interacts with cullin-associated NEDD8-dissociated protein 1 (CAND1), nuclear gene transcription assembly SCF ubiquitin ligase complexes. A functional role this interaction was also identified, since lower levels were observed cells decreased CAND1 expression via...
The vitamin D and microRNA (miR) systems may play a role in the pathogenesis of cardiometabolic disorders, including hypertension. HYPODD study was double-blind placebo-controlled trial aiming to assess effects cholecalciferol treatment patients with well-controlled hypertension hypovitaminosis (25OHD levels < 50 nmol/L). In addition this clinical trial, we also evaluated calcitriol on miR-21 expression vivo vitro, respectively. Changes cardiovascular risk profiles were treated (C-cohort)...
The human carbonic anhydrase IX (CA IX) is a hypoxia-induced transmembrane protein belonging to the α-CA enzyme family. It has crucial role in pH regulation hypoxic cells and acts by buffering intracellular acidosis induced hypoxia. Indeed, it frequently expressed cancer cells, where contributes tumor progression. CA also able localize nucleus, 47S rRNA precursor genes transcription; however, mechanisms assisting its nuclear translocation still remain unclear. aim of our study was deepen...
The 10q24.33 locus is known to be associated with susceptibility cutaneous malignant melanoma (CMM), but the mechanisms underlying this association have been not extensively investigated. We carried out an integrative genomic analysis of using epigenomic annotations and in vitro reporter gene assays identify regulatory variants. found two putative functional single nucleotide polymorphisms (SNPs) enhancer promoter OBFC1, respectively, neural crest CMM cells, one, rs2995264, altering...
The PID1/NYGGF4/PCLI1 gene encodes for a protein with phosphotyrosine-binding domain, which interacts the lipoprotein receptor-related 1. Previous work by us and others suggested function of in cell proliferation NIH3T3 fibroblasts 3T3-L1 pre-adipocytes. molecular characterization PCLI1 protein, ectopically expressed fibroblasts, revealed two phosphorylation sites at Ser154 Ser165. In order to clarify functions this gene, we analyzed effects its downregulation on cellular cycle progression...
Normal cells do not replicate indefinitely, rather after a certain number of divisions, they enter into state permanent arrest proliferation defined cellular senescence. Cellular senescence is accompanied by reprogramming gene expression profile, resulting in changes the regulatory mechanisms that act at both transcriptional and post-trascriptional as well epigenetic level. MicroRNAs (miRs) are small non-coding RNAs play fundamental role negative regulation post-transcriptional level. They...