A5078975633- Platelet Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Electrospun Nanofibers in Biomedical Applications
- Cell Adhesion Molecules Research
- Neuroscience and Neural Engineering
- Mesenchymal stem cell research
- Blood groups and transfusion
- Periodontal Regeneration and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cardiac Structural Anomalies and Repair
- Silk-based biomaterials and applications
- Chronic Myeloid Leukemia Treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Liver Disease Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Fibroblast Growth Factor Research
- Eosinophilic Disorders and Syndromes
- Adenosine and Purinergic Signaling
- Corneal Surgery and Treatments
- Proteoglycans and glycosaminoglycans research
- Blood properties and coagulation
- Protease and Inhibitor Mechanisms
Enzo Life Sciences (United States)
2022
Adarza BioSystems (United States)
2018-2020
New York Stem Cell Foundation
2015-2017
University of Pavia
2005-2014
Istituti di Ricovero e Cura a Carattere Scientifico
2008-2013
Tufts University
2009-2013
Recent advances in techniques to differentiate human induced pluripotent stem cells (hiPSCs) hold the promise of an unlimited supply derived cardiac from both healthy and disease populations. That has been tempered by observation that hiPSC-derived cardiomyocytes (hiPSC-CMs) typically retain a fetal-like phenotype, raising concern about translatability vitro data obtained drug safety, discovery, development studies. The Biowire II platform was used generate 3D engineered tissues (ECTs)...
Background The mechanism by which megakaryocytes (Mks) proliferate, differentiate, and release platelets into circulation are not well understood. Growing evidence indicates that a complex regulatory mechanism, involving cellular interactions, composition of the extracellular matrix physical parameters such as oxygen tension, may contribute to quiescent or permissive microenvironment related Mk differentiation maturation within bone marrow. Methodology/Principal Findings Differentiating...
Platelets are specialized cells produced by megakaryocytes in the bone marrow that represent first defense against hemorrhage, yet they also play a pathological role thrombosis, inflammation, and cancer. Millions of platelet transfusions conducted each year, supply this blood component is limited. There many diseases where production or function impaired with severe consequences for patients. With such clinical need, new insight into formation platelets would have major impact on patients...
Background The interaction of adenosine diphosphate with its P2Y1 and P2Y12 receptors on platelets is important for platelet function. However, nothing known about function in human megakaryocytes.Design Methods We studied the role P2Y proplatelet formation by megakaryocytes culture.Results Megakaryocytes expressed all eight subtypes receptors, constitutively released diphosphate. Proplatelet was inhibited scavengers apyrase CP/CPK 60-70% inhibitors cangrelor 2-MeSAMP 50-60%, but not...
Megakaryocytes release platelets into the bloodstream by elongating proplatelets. In this study, we showed that human megakaryocytes constitutively Transforming Growth Factor β1 and express its receptors. Importantly, downstream signaling, through SMAD2/3 phosphorylation, was shown to be active in extending proplatelets, indicating a type of autocrine stimulation on megakaryocyte development. Furthermore, inactivation receptor inhibitors SB431542 Stemolecule ALK5 inhibitor, determined...
Safety attrition due to drug-induced inotropic changes remains a significant risk factor for drug development. Mitigating these events during early screening challenging. Several in vitro predictive models have been developed address issues, with varying success detecting changes. In this study, we compared traditional two-dimensional human-induced pluripotent stem cell-derived cardiomyocytes (2D hiPSC-CMs) three-dimensional engineered cardiac tissues (3D ECTs) assess their ability detect 17...
Following cardiac injury, the ischaemic heart tissue is characterized by invasion of pro-inflammatory (M1) and pro-healing (M2) macrophages. Any engineered will inevitably interact with inflammatory environment found at site myocardial infarction time implantation. However, interactions between repair cells remain poorly understood. Here we recapitulated in vitro some important cellular events such as macrophage recruitment their effect on differentiation maturation, taking into account...
Cardiac contractility modulation (CCM) is a medical device therapy whereby non-excitatory electrical stimulations are delivered to the myocardium during absolute refractory period enhance cardiac function. We previously evaluated effects of standard CCM pulse parameters in isolated rabbit ventricular cardiomyocytes and 2D human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) monolayers, on flexible substrate. In present study, we sought extend these results 3D...
One of the main efforts in myocardial tissue engineering is towards designing cardiac tissues able to rescue reduction heart function once implanted at site infarction. To date, efficiency this approach preclinical applications limited part by our incomplete understanding inflammatory environment known be present infarct and poor vascularization. It was recently reported that polarized macrophages infarction secrete bone morphogenetic proteins (BMPs)-2 -4 causing changes expression a 2D...