A5058998346- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Endometriosis Research and Treatment
- Maternal and fetal healthcare
- Pregnancy and Medication Impact
- Preterm Birth and Chorioamnionitis
- Prenatal Screening and Diagnostics
- Uterine Myomas and Treatments
- Reproductive Physiology in Livestock
- Gestational Trophoblastic Disease Studies
- Ectopic Pregnancy Diagnosis and Management
- Malaria Research and Control
- Birth, Development, and Health
- COVID-19 Impact on Reproduction
- Cell Adhesion Molecules Research
- Vitamin D Research Studies
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Cardiovascular Issues in Pregnancy
- Global Maternal and Child Health
- Monoclonal and Polyclonal Antibodies Research
- Endometrial and Cervical Cancer Treatments
- Gynecological conditions and treatments
- Gestational Diabetes Research and Management
- Galectins and Cancer Biology
Newcastle University
2013-2022
Royal Victoria Infirmary
2006-2021
Guangzhou Medical University
2019
Guangzhou Women and Children Medical Center
2019
University of Newcastle Australia
1992-2017
University of Glasgow
1999-2013
Institute of Genetics and Cancer
2013
Sunderland Royal Hospital
2004
University of Birmingham
2004
Queen Elizabeth Hospital Birmingham
2004
Granulated lymphocytes with an unusual antigenic phenotype (CD56 + CD38 CD2 CD3 - CD16 -) form a substantial proportion of leukocytes in human endometrial stroma. The purpose this study was to examine morphological and anti- genie heterogeneity granulated (eGL) imprint preparations, paraffin-embedded sections frozen sections. eGL decidual imprints showed varia tions cell size, nuclear shape chromatin content the number size cytoplasmic granules. were detected using phloxine tartrazine,...
Failure to transform uteroplacental spiral arteries is thought underpin disorders of pregnancy, including preeclampsia and fetal growth restriction (FGR). In this study, artery remodeling extravillous-cytotrophoblast were examined in placental bed biopsies from normal pregnancy (n = 25), 22), severe FGR 10) then compared with clinical parameters. Biopsies immunostained determine vessel wall integrity, location/density, periarterial fibrinoid, endothelium. Muscle disruption was reduced...
Abstract Remodeling of uterine spiral arteries is critical for the continuation a successful pregnancy. Uterine natural killer (uNK) cells are predominant leukocyte population in early pregnant decidua, and role these artery remodeling pregnancy has been suggested. Angiogenic growth factors were measured isolated uNK total (unseparated) decidual (8–10 or 12–14 weeks gestation, n=5 each gestational age) after culture 48 h. Angiopoietin (Ang)1, placental factor, transforming factor-β1...
Uterine spiral artery remodeling is required for successful human pregnancy; impaired associated with pregnancy complications, including late miscarriage, preeclampsia, and fetal growth restriction. The molecular triggers of are not known, but it now clear that there “trophoblast-independent” “trophoblast-dependent” stages. natural killer (uNK) cells abundant in decidualized endometrium early they surround arteries secrete a range angiogenic factors. We hypothesized uNK mediate the initial...
Increased numbers of phenotypically unusual CD56(bright) CD16- uterine natural killer (uNK) cells have been associated with recurrent reproductive failure. uNK produce angiogenic growth factors and are potential regulators decidual angiogenesis in early pregnancy. The final common mechanism for pregnancy loss is thought to be onset the maternal circulation excessive placental oxidative stress. We tested hypothesis that increased preimplantation endometrium altered angiogenesis.Women failure...
The active form of vitamin D, 1,25-dihydroxyvitamin D(3) (1,25[OH](2)D(3)) is a potent immunomodulatory seco-steroid. We have demonstrated that several components D metabolism and signaling are strongly expressed in human uterine decidua from first trimester pregnancies, suggesting locally produced 1,25(OH)(2)D(3) may exert immunosuppressive effects during early stages gestation. To investigate this further, we used primary cultures decidual cells third pregnancies to demonstrate expression...
Pregnant women are more likely to contract malaria than their non‐pregnant counterparts. The aim of this study was develop a simple classification system for the histopathological diagnosis placental infection applicable placentas collected in field conditions. were classified into four groups depending on presence and disribution parasites pigment: active infection, active‐chronic past‐chronic not infected. frequency parasitized (26.4%) keeping with prevalence parasitaemia documented...
Objective To assess the incidence and distribution of both oestrogen progesterone receptors throughout female lower urinary tract, to compare receptor expression in women varying status. Patients methods The study included 90 undergoing surgery for urogynaecological conditions; 33 were premenopausal, 26 postmenopausal taking no supplementation, 31 receiving some form hormone‐replacement therapy. Biopsies taken during from bladder dome, trigone, proximal urethra, distal vagina vesicovaginal...
Successful remodeling of the uterine spiral arteries is essential for a complication-free pregnancy and best described in terms its morphologic features. The molecular mediators cellular sources artery are not known, although role leukocytes has been proposed. Immunohistochemical assessment placental bed biopsies demonstrated NK cells, macrophages, T lymphocytes wall adventitia at different stages remodeling, regardless presence extravillous trophoblast cells. Leukocytes were more prevalent...
Alterations in the balance of leucocyte populations uterine decidua may lead to generation an unfavourable cytokine environment that is associated with unsuccessful pregnancy. Single and double immunohistochemical labelling was used examine from normal third trimester, foetal growth-restricted pre-eclamptic pregnancies. Placental bed biopsies 12 women undergoing elective Caesarean section no hypertension or growth restriction (FGR), 8 FGR without maternal pre-eclampsia (PE) were quantify...
In clinical trials, maternal tetanus toxoid (TT) vaccination is effective in protecting newborns against infection, but inadequate placental transfer of antibodies may contribute to lower-than-expected rates protection routine practice. We studied the effect malaria and human immunodeficiency virus (HIV) infection on tetanus.A total 704 maternal-cord paired serum samples were tested by ELISA for tetanus. The HIV status all women was determined an immunoglobulin G antibody-capture...
Extravillous trophoblast (EVT) cell invasion of uterine decidua and the inner third myometrium is critical for successful pregnancy. Many decidual factors are likely to play a role in regulating this process. We have previously shown that cytokines, known be produced by natural killer (uNK) cells, such as TNF-alpha, TGF-beta1 IFN-gamma inhibit EVT invasion. therefore hypothesized supernatants from purified uNK cells would invasion.Total unfractionated 8 10 weeks gestation increased placental...
In addition to its role in the prevention of neural tube defects, folic acid has many other physiological functions, including cell proliferation, DNA replication, and antioxidant protection. The aim this study was determine that regulating placental trophoblast development. Placental explants from placentae at gestational age 7 wk (n = 3) were cultured concentrations 10(-6) M, 10(-8) 10(-10) M. Extravillous (EVT) invasion assessed following 6-day culture, used for immunohistochemical...