A5062497655- Aquatic Ecosystems and Biodiversity
- Click Chemistry and Applications
- Environmental Toxicology and Ecotoxicology
- Computational Drug Discovery Methods
- Effects and risks of endocrine disrupting chemicals
- Pharmaceutical and Antibiotic Environmental Impacts
- Monoclonal and Polyclonal Antibodies Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Fecal contamination and water quality
- Chemical Synthesis and Analysis
- Neurobiology and Insect Physiology Research
- Child Nutrition and Water Access
- Toxic Organic Pollutants Impact
- Chemical Analysis and Environmental Impact
- Circadian rhythm and melatonin
- Per- and polyfluoroalkyl substances research
- Molecular Biology Techniques and Applications
- Evolution and Genetic Dynamics
- Vitamin D Research Studies
- Physiological and biochemical adaptations
- Microplastics and Plastic Pollution
- Aquaculture disease management and microbiota
- Carcinogens and Genotoxicity Assessment
- Vitamin K Research Studies
- Thyroid Disorders and Treatments
Oak Ridge Institute for Science and Education
2023-2025
Environmental Protection Agency
2022-2025
Oak Ridge Associated Universities
2023-2025
Research Triangle Park Foundation
2025
North Carolina State University
2015-2019
Baylor University
2011
Aquatic Systems (United States)
2011
The Tox24 challenge was designed to evaluate the progress that has been made in computational method development for prediction of vitro activity since Tox21 challenge. In this challenge, participants were tasked with developing models predict chemical binding transthyretin (TTR), a serum protein, based on structure. analyzed dataset included chemicals screened competitive assay measure reduction fluorescence due displacement 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANSA) from...
In recent years pharmaceuticals have been detected in aquatic systems receiving discharges of municipal and industrial effluents. Although diphenhydramine (DPH) has reported water, sediment, fish tissue, an understanding its impacts on organisms is lacking. Diphenhydramine multiple modes action (MOA) targeting the histamine H1, acetylcholine (ACh), 5-HT reuptake transporter receptors, as such used hundreds pharmaceutical formulations. The primary objective this study was to develop a...
Transthyretin (TTR) is one of the serum binding proteins responsible for transport thyroid hormones (TH) to target tissue and maintaining balance available TH. Chemical TTR subsequent displacement TH has been identified as an end point in screening chemicals potential disruption system. To address lack data regarding TTR, we optimized
New approach methodologies (NAMs) are an increasing priority in the field of toxicology to fill data gaps and reduce time resources chemical safety assessment. We describe NAMs workflow that integrates vitro high-throughput bioassay with silico computational model. In defining this workflow, we propose, as a crucial step development, identification explicit "purpose contexts": priori definitions scope intent solution, which provide natural targets for mechanistic interpretation, validation,...
ABSTRACT Microplastics (MPs) are known vectors for the transport of pathogens and antibiotic resistance genes (ARGs), but few studies have examined long‐term (> 30 days) development MP biofilms. Wastewater Treatment Plant (WWTP) effluents a significant source MPs, pathogens, antibiotics released into environment. We explored biofilms on high‐ low‐density polyethylene, polypropylene, polystyrene incubated in an experimental flow‐through stream facility over course 10 weeks. Treatments...
The Tox24 challenge was designed to evaluate the progress that has been made in computational method development for prediction of vitro activity since Tox21 challenge. In this challenge, participants were tasked with developing models predict chemical binding transthyretin (TTR), a serum protein, based on structure. analyzed dataset included chemicals screened competitive assay measure reduction fluorescence due displacement 8-anilino-1-naphthalenesulfonic acid ammonium salt (ANSA) from...
Transthyretin (TTR) is one of the serum binding proteins responsible for transport thyroid hormones (TH) to target tissue and maintaining balance available TH. Chemical TTR subsequent displacement TH has been identified as an endpoint in screening chemicals potential disruption system. To address lack data regarding TTR, we optimized vitro assay utilizing fluorescent probe 8-anilino-1-napthalenesulfonic acid (ANSA) human protein screen over 1,500 from U.S. EPA’s ToxCast ph1_v2, ph2, e1k...
Nitrogenous compounds enter the environment through various anthropogenic sources. Among these are nitrate (NO
Transthyretin (TTR) is one of the serum binding proteins responsible for transport thyroid hormones (TH) to target tissue and maintaining balance available TH. Chemical TTR subsequent displacement TH has been identified as an endpoint in screening chemicals potential disruption system. To address lack data regarding TTR, we optimized vitro assay utilizing fluorescent probe 8-anilino-1-napthalenesulfonic acid (ANSA) human protein screen over 1,500 from U.S. EPA’s ToxCast ph1_v2, ph2, e1k...
Thyroid hormone (TH) carrier proteins play an important role in distributing TH to target tissue as well maintaining the balance of free versus bound blood. Interference with has been identified a potential mechanism thyroid system disruption. To address lack data regarding chemicals binding these and displacing TH, fluorescence-based
Biological rhythms regulate innumerable physiological processes, yet little is known of factors that many these rhythms. Disruption in the timing can have devastating impacts on population sustainability. We hypothesized molt infradian rhythm crustacean Daphnia magna regulated by joint action protein E75 and nitric oxide. Further, we disruption function would adversely impact several processes related to growth reproduction. Analysis mRNA levels genes, involved regulating cycle insects,...
The high throughput screening of chemicals for interaction with intracellular targets is gaining prominence in the toxicity evaluation environmental chemicals. We describe ligand-mediated receptor assembly as an early event signaling and its application to targeted receptors. utilized bioluminescence resonance energy transfer (BRET) detect quantify methyl farnesoate (MfR) response various high-production volume other hormone binds MfR regulate aspects reproduction development crustaceans....