A5005084773- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- Lipid Membrane Structure and Behavior
- Calcium signaling and nucleotide metabolism
- Cardiovascular Disease and Adiposity
- Gestational Diabetes Research and Management
- Mesenchymal stem cell research
- Birth, Development, and Health
- Biotin and Related Studies
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Lipid metabolism and biosynthesis
- Pregnancy and preeclampsia studies
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Diet and metabolism studies
- Mitochondrial Function and Pathology
- Growth Hormone and Insulin-like Growth Factors
- Peroxisome Proliferator-Activated Receptors
- Bariatric Surgery and Outcomes
- RNA Research and Splicing
University of Massachusetts Chan Medical School
2016-2025
Morningside College
2023
Massachusetts Academy of Math and Science
2023
National Institutes of Health
2020
Yale University
2010-2020
Harvard University
1986-2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Cornell University
2020
Princeton University
2020
Adipose tissue plays a central role in the control of energy homeostasis through storage and turnover triglycerides secretion factors that affect satiety fuel utilization. Agents enhance insulin sensitivity, such as rosiglitazone, appear to exert their therapeutic effect adipose tissue, but precise mechanisms actions are unclear. Rosiglitazone changes morphological features protein profiles mitochondria 3T3-L1 adipocytes. To examine relevance these effects vivo, we studied white adipocytes...
Phagosomal biogenesis is a fundamental biological process of particular significance for the function phagocytic and antigen-presenting cells. The precise mechanisms governing maturation phagosomes into phagolysosomes are not completely understood. Here, we applied property pathogenic mycobacteria to cause phagosome arrest in infected macrophages as tool dissect critical steps phagosomal biogenesis. We report requirement 3-phosphoinositides acquisition Rab5 effector early endosome...
White adipose tissue is an important endocrine organ involved in the control of whole-body metabolism, insulin sensitivity, and food intake. To better understand these functions, 3T3-L1 cell differentiation was studied by using combined proteomic genomic strategies. The proteomics approach developed here exploits velocity gradient centrifugation as alternative to isoelectric focusing for protein separation first dimension. A 20- 30-fold increase concentration numerous mitochondrial proteins...
Storage of energy as triglyceride in large adipose-specific lipid droplets is a fundamental need all mammals. Efficient sequestration fat adipocytes also prevents fatty acid overload skeletal muscle and liver, which can impair insulin signaling. Here we report that the Cide domain-containing protein Cidea, previously thought to be mitochondrial protein, colocalizes around with perilipin, regulator lipolysis. Cidea-GFP greatly enhances droplet size when ectopically expressed preadipocytes or...
Background— Adipose tissue expands in response to excess caloric intake, but individuals prone deposit visceral instead of subcutaneous adipose have higher risk metabolic disease. The role angiogenesis the expandability human depots is unknown. objective this study was measure and establish whether there a relationship between obesity, status, angiogenic properties these depots. Methods Results— Angiogenic capacity determined by quantifying capillary branch formation from explants embedded...
Transforming growth factor (TGF)β is an important physiological regulator of cellular and differentiation. It activates a receptor threonine/serine kinase that phosphorylates the transcription Smad2, which then translocates into nucleus to trigger specific transcriptional events. Here we show activated type I II TGFβ receptors internalize endosomes containing early endosomal protein EEA1. The extent TGFβ-stimulated Smad2 phosphorylation, nuclear translocation, correlated closely with...
Human platelet-derived growth factor receptors (PDGFRs) expressed in human Hep G2 cells internalized and concentrated a juxtanuclear region near the Golgi network within 10 minutes after were treated with PDGF. A PDGFR mutant (F5) that lacks high-affinity binding sites for Src homology 2 domain-containing proteins phosphatidylinositol-3 kinase (PI-3 kinase), Ras guanosine triphosphatase activating protein, phospholipase C-γ, phosphotyrosine phosphatase (Syp) remained at cell periphery....
Insulin stimulates the accumulation of iron by isolated fat cells increasing uptake diferric transferrin. Analysis cell-surface binding 125I-transferrin indicated that insulin caused a 3-fold increase in cell surface number transferrin receptors. This result was confirmed demonstration increases an anti-rat receptor monoclonal antibody (OX-26) to cells. The basis this effect examined investigating receptors membrane fractions from disrupted Two methods were employed. First isotherm membranes...
Phosphatidylinositol 3-kinases (PI 3-kinases) have been implicated in membrane trafficking the secretory and endocytic pathways of yeast mammalian cells, but molecular mechanisms by which these lipid kinases operate are not known. Here we identify a protein 170 kDa that is rapidly released from cell membranes response to wortmannin, potent inhibitor PI 3-kinases. The amino acid sequence peptides p170 reveal its identity early endosomal antigen (EEA) 1, an with homology several proteins...
PPARgamma activators such as rosiglitazone (RSG) stimulate adipocyte differentiation and increase subcutaneous adipose tissue mass. However, in addition to preadipocyte differentiation, expansion requires neovascularization support increased numbers. Paradoxically, endothelial cell growth is potently inhibited by RSG vitro, raising the question of how this drug can induce an mass while inhibiting angiogenesis. We find that from mice treated with have capillary density. To determine whether...
Single-cell sequencing technologies have revealed an unexpectedly broad repertoire of cells required to mediate complex functions in multicellular organisms. Despite the multiple roles adipose tissue maintaining systemic metabolic homeostasis, adipocytes are thought be largely homogenous with only 2 major subtypes recognized humans so far. Here we report existence and characteristics 4 distinct human adipocyte subtypes, their respective mesenchymal progenitors. The phenotypes these...