A5013803913- Genomics and Phylogenetic Studies
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Environmental DNA in Biodiversity Studies
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
Rice University
2022-2025
Oregon Health & Science University
2018-2019
Abstract Individual RNA remains a challenging signal to synthetically transduce into different types of cellular information. Here, we describe Ribozyme-ENabled Detection (RENDR), plug-and-play strategy that uses transcripts template the assembly split ribozymes, triggering splicing reactions generate orthogonal protein outputs. To identify ribozymes require templating for splicing, use laboratory evolution evaluate activities variants Tetrahymena thermophila ribozyme. The best design...
The matrix (MA) domains of HIV-1 precursor Gag (PrGag) proteins direct PrGag to plasma membrane (PM) assembly sites where envelope (Env) protein trimers are incorporated into virus particles. MA targeting PM is facilitated by its binding phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2], and cellular RNAs appears serve a chaperone function that prevents from associating with intracellular membranes prior arrival at the PI(4,5)P2-rich PM. Investigations have shown genetic evidence an...
ABSTRACT Biological recorders can code information in DNA, but they remain challenging to apply complex microbial communities. To program microbiome storage, a synthetic catalytic RNA (cat-RNA) was used write ribosomal (rRNA) about gene transfer host range. By reading out native and modified rRNA using amplicon sequencing, we find that 140 of 279 wastewater community members from twenty taxonomic orders participate conjugation observe differences storage across sequence variants. Twenty the...
ABSTRACT Individual RNA remains a challenging signal to synthetically transduce into different types of cellular information. Here, we describe Ribozyme-ENabled Detection (RENDR), plug-and-play strategy that uses transcripts template the assembly split ribozymes, triggering splicing reactions generate orthogonal protein outputs. To identify ribozymes require templating for splicing, used laboratory evolution evaluate activities variants Tetrahymena thermophila ribozyme. The best design...