A5057173635- Congenital heart defects research
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Congenital Diaphragmatic Hernia Studies
- Congenital Anomalies and Fetal Surgery
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Environmental DNA in Biodiversity Studies
Rice University
2023-2025
Massachusetts General Hospital
2021-2023
Bacteriophages (phages) facilitate gene transfer and microbial evolution in all ecosystems have applications as tools for engineering microbiomes antimicrobials. Historic efforts to map phage hosts, such plaque assays, are limited culturable bacteria, low throughput, hard apply environmentally-relevant contexts. To overcome these limitations, a synthetic ribozyme that stores information about DNA uptake 16S ribosomal RNA (rRNA) was used identify phage-host interactions by integrating the...
Microbes can be programmed to record participation in gene transfer by coding biological-recording devices into mobile DNA. Upon DNA uptake, these transcribe a catalytic RNA (cat-RNA) that binds conserved sequences within ribosomal (rRNA) and perform trans-splicing reaction adds barcode the rRNA. Existing cat-RNA designs were manually generated broad-host range, providing no control over organisms barcoded. To achieve barcoded cat-RNA, we created program called Ribodesigner uses input sets...
ABSTRACT Biological recorders can code information in DNA, but they remain challenging to apply complex microbial communities. To program microbiome storage, a synthetic catalytic RNA (cat-RNA) was used write ribosomal (rRNA) about gene transfer host range. By reading out native and modified rRNA using amplicon sequencing, we find that 140 of 279 wastewater community members from twenty taxonomic orders participate conjugation observe differences storage across sequence variants. Twenty the...
ABSTRACT Congenital diaphragmatic hernia (CDH) is a relatively common and genetically heterogeneous structural birth defect associated with high mortality morbidity. We describe eight unrelated families novel X-linked condition characterized by diaphragm defects, variable anterior body wall anomalies, and/or facial dysmorphism. Using linkage analysis whole exome or genome sequencing, we identified missense variants in the actin binding domains of plastin 3 ( PLS3 ), gene encoding an bundling...