A5035578646- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Animal Genetics and Reproduction
- Bacterial Genetics and Biotechnology
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- Gene Regulatory Network Analysis
- Genomics and Phylogenetic Studies
Rice University
2025
University of Utah
2019-2021
Microbes can be programmed to record participation in gene transfer by coding biological-recording devices into mobile DNA. Upon DNA uptake, these transcribe a catalytic RNA (cat-RNA) that binds conserved sequences within ribosomal (rRNA) and perform trans-splicing reaction adds barcode the rRNA. Existing cat-RNA designs were manually generated broad-host range, providing no control over organisms barcoded. To achieve barcoded cat-RNA, we created program called Ribodesigner uses input sets...
Expanding the genetic toolbox for prokaryotic synthetic biology is a promising strategy enhancing dynamic range of gene expression and enabling new engineered applications research biomedicine. Here, we reverse current trend moving parts from prokaryotes to eukaryotes demonstrate that activating eukaryotic transcription factor QF its corresponding DNA-binding sequence can be moved E. coli introduce transcriptional activation, in addition tight off states. We further used devices respond low...
Synthetic biology is a relatively new field of science that combines aspects and engineering to create novel tools for the construction biological systems. Using within synthetic biology, stem cells can then be reprogrammed differentiated into specified cell type. Stem have already proven largely beneficial in many different therapies paved way tissue regenerative medicine. Although scientists made great strides engineering, there still remain questions answered regard regeneration....