A5049078756- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Advanced Breast Cancer Therapies
- Telomeres, Telomerase, and Senescence
- Cytomegalovirus and herpesvirus research
- Acute Myeloid Leukemia Research
- Cancer-related Molecular Pathways
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
- Neutropenia and Cancer Infections
- Transgenic Plants and Applications
- Genetic diversity and population structure
- Reproductive tract infections research
- Beetle Biology and Toxicology Studies
- Pediatric Urology and Nephrology Studies
- Dermatology and Skin Diseases
- Acute Lymphoblastic Leukemia research
- Kidney Stones and Urolithiasis Treatments
- Monoclonal and Polyclonal Antibodies Research
- Chronic Myeloid Leukemia Treatments
University of Southampton
2014-2025
Cancer Research UK
2011-2022
NIHR CRUK Experimental Cancer Medicine Centre
2022
Southampton General Hospital
2019
Genomics (United Kingdom)
2016
Royal Bournemouth Hospital
2011-2013
St James's University Hospital
2008-2011
Abstract The value of genome-wide over targeted driver analyses for predicting clinical outcomes cancer patients is debated. Here, we report the whole-genome sequencing 485 chronic lymphocytic leukemia enrolled in trials as part United Kingdom’s 100,000 Genomes Project. We identify an extended catalog recurrent coding and noncoding genetic mutations that represents a source future studies provide most complete high-resolution map structural variants, copy number changes global genome...
ATM mutation and BIRC3 deletion and/or have independently been shown to prognostic significance in chronic lymphocytic leukemia. However, the relative clinical importance of these abnormalities patients with a 11q encompassing gene has not established. We screened cohort 166 enriched for 11q-deletions mutations determined overall progression-free survival among 133 cases treated within UK LRF CLL4 trial. SNP6.0 profiling demonstrated that occurred 83% 11q-deleted always co-existed deletion....
The pathogenesis of splenic marginal zone lymphoma (SMZL) remains largely unknown. Recent high-throughput sequencing studies have identified recurrent mutations in key pathways, most notably NOTCH2 >25% patients. These are based on small, heterogeneous discovery cohorts, and therefore only captured a fraction the lesions present SMZL genome. To identify further novel pathogenic within related biochemical we applied whole exome (WES) copy number (CN) analysis to biologically clinically...
Rapid, high throughput diagnostics are a valuable tool, allowing the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in populations so as to identify and isolate people with asymptomatic symptomatic infections. Reagent shortages restricted access testing solutions have limited effectiveness conventional assays such quantitative RT-PCR (RT-qPCR), particularly throughout first months disease 2019 pandemic. We investigated use LamPORE, where loop-mediated isothermal...
Abstract Despite advances in chronic lymphocytic leukaemia (CLL) treatment, globally chemotherapy remains a central treatment modality, with trials representing an invaluable resource to explore disease-related/genetic features contributing long-term outcomes. In 499 LRF CLL4 cases, trial >12 years follow-up, we employed targeted resequencing of 22 genes, identifying 623 mutations. After background mutation rate correction, 11/22 genes were recurrently mutated at frequencies between 3.6%...
The biological features of IGHV-M chronic lymphocytic leukemia responsible for disease progression are still poorly understood. We undertook a longitudinal study close to diagnosis, pre-treatment and post relapse in 13 patients presenting with cMBL or Stage A good-risk biomarkers (IGHV-M genes, no del(17p) del(11q) low CD38 expression) who nevertheless developed progressive disease, whom 10 have required therapy. Using cytogenetics, fluorescence situ hybridisation, genome-wide DNA...
Background The skin microbiome is increasingly recognised as a critical component to the pathogenesis of many inflammatory disorders including atopic dermatitis. However, few studies have looked in detail at impact changing both sampling methodology and 16S rRNA hypervariable region sequencing tandem for analysis. Methods We set out undertake detailed analysis comparing microbiota population diversity composition using swab, tape, scrape then swab assayed by two regions. Comparisons were...
Abstract Chronic lymphocytic leukemia patients with mutated immunoglobulin heavy-chain genes (IGHV-M), particularly those lacking poor-risk genomic lesions, often respond well to chemoimmunotherapy (CIT). DNA methylation profiling can subdivide early-stage into naive B-cell–like CLL (n-CLL), memory (m-CLL), and intermediate (i-CLL), differing times first treatment overall survival. However, whether identify destined favorably CIT has not been ascertained. We classified treatment-naive (n =...
<ns3:p><ns3:bold>Background:</ns3:bold><ns3:italic> Chlamydia trachomatis</ns3:italic> is a prolific human pathogen that can cause serious long-term conditions if left untreated. Recent developments in <ns3:italic>Chlamydia</ns3:italic> genetics have opened the door to conducting targeted and random mutagenesis experiments identify gene function. In present study, an inducible transposon approach was developed for <ns3:italic>C. using self-replicating vector deliver transposon-transposase...
Next-generation sequencing (NGS) has transitioned from research to clinical routine, yet the comparability of different technologies for mutation profiling remains an open question. We performed a European multicenter (n=6) evaluation three amplicon-based NGS assays targeting 11 genes recurrently mutated in chronic lymphocytic leukemia. Each assay was assessed by two centers using 48 pre-characterized leukemia samples; libraries were sequenced on Illumina MiSeq instrument and bioinformatics...
Abstract The Eurasian red squirrel ( Sciurus vulgaris ) is an emblematic species for conservation, and its decline in the British Isles exemplifies impact that alien introductions can have on native ecosystems. Indeed, squirrels this region declined dramatically over last 60 years due to spread of squirrelpox virus following introduction gray carolinensis ). Currently, populations Britain are fragmented need be closely monitored order assess their viability effectiveness conservation...
Abstract Chronic lymphocytic leukemia (CLL) cells have variably low surface IgM (sIgM) levels/signaling capacity, influenced by chronic antigen engagement at tissue sites. Within these levels, CLL with relatively high sIgM (CLLhigh) progresses more rapidly than (CLLlow). During ibrutinib therapy, surviving redistribute into the peripheral blood and can recover expression. Return of to may eventually recur, where could promote tumor growth. We analyzed time new treatment (TTNT) following in...
Abstract Background The skin microbiome is increasingly recognised as a critical component of the innate immune response and central to pathogenesis many inflammatory disorders including atopic dermatitis. Previous studies have not looked in detail at impact changing both sampling methodology hypervariable region sequencing for analysis. Objectives We set out undertake detailed analysis comparing microbe population diversity resolution single species level by swab, tape, scrape then swab...
Introduction: Rapid, high throughput diagnostics are a valuable tool, allowing the detection of SARS-CoV-2 in populations, order to identify and isolate people with asymptomatic symptomatic infections. Reagent shortages restricted access testing solutions have limited effectiveness conventional assays such as reverse transcriptase quantitative PCR (RTqPCR), particularly throughout first months pandemic. We investigated use LamPORE, where loop mediated isothermal amplification (LAMP) is...