A5082142068- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Polyamine Metabolism and Applications
- RNA Research and Splicing
- Fungal Infections and Studies
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Neurogenetic and Muscular Disorders Research
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA regulation and disease
- Peptidase Inhibition and Analysis
- Fermentation and Sensory Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Amyotrophic Lateral Sclerosis Research
- Biochemical and Molecular Research
- Yeasts and Rust Fungi Studies
George Washington University
2021
Université de Montréal
2021
Centre Hospitalier de l’Université de Montréal
2021
Concordia University
2015-2017
Boston Children's Hospital
2016
Harvard University
2016
Invasive fungal infections are a leading cause of human mortality. Effective treatment is hindered by the rapid emergence resistance to limited number antifungal drugs, demanding new strategies treat life-threatening infections. Here, we explore powerful strategy enhance efficacy against pathogens using natural product beauvericin. We found that beauvericin potentiates activity azole antifungals azole-resistant Candida isolates via inhibition multidrug efflux and itself effluxed Yor1. As...
Cytoplasmic stress granules (SGs) are dynamic foci containing translationally arrested mRNA and RNA-binding proteins (RBPs) that form in response to a variety of cellular stressors. It has been debated SGs may evolve into cytoplasmic inclusions observed many neurodegenerative diseases. Recent studies have examined the SG proteome by interrogating interactome G3BP1. However, it is widely accepted multiple baits required capture full proteome. To gain further insight proteome, we employed...
The zinc cluster transcription factor Put3 was initially characterized in Saccharomyces cerevisiae as the transcriptional activator of PUT1 and PUT2, two genes acting early proline assimilation pathway. We have used phenotypic studies, profiling, chromatin immunoprecipitation with microarray technology (ChIP-chip) to establish that unlike S. cerevisiae, which only uses a nitrogen source, Candida albicans can use carbon or source both carbon. However, C. put3 null mutant cannot grow on...
Abstract Zinc cluster transcription factors (TFs) are essential fungal regulators of gene expression. In the pathogen Candida albicans, orf19.1604 encodes a zinc TF regulating filament development. Hyperactivation orf19.1604, which we have named RHA1 for Regulator Hyphal Activity, generates wrinkled colony morphology under nonhyphal growth conditions, triggers formation, invasiveness, and enhanced biofilm formation causes reduced virulence in mouse model systemic infection. The strain...
Abstract Cytoplasmic stress granules (SGs) are dynamic non-membranous foci containing translationally arrested mRNA and RNA binding proteins that form in response to a variety of cellular stressors. SGs may evolve into the cytoplasmic inclusions observed many neurodegenerative diseases. Recent studies have examined SG proteome by interrogating interactome G3BP1, core protein. To gain further insight proteome, we employed an immunoprecipitation coupled with mass spectrometry approach...