A5078463038- Legionella and Acanthamoeba research
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antifungal resistance and susceptibility
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fungal Biology and Applications
- Fungal and yeast genetics research
- Biotin and Related Studies
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Heme Oxygenase-1 and Carbon Monoxide
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
University of Toronto
2016-2024
University of Alberta
2019
Alberta Health Services
2019
Invasive fungal infections are a leading cause of human mortality. Effective treatment is hindered by the rapid emergence resistance to limited number antifungal drugs, demanding new strategies treat life-threatening infections. Here, we explore powerful strategy enhance efficacy against pathogens using natural product beauvericin. We found that beauvericin potentiates activity azole antifungals azole-resistant Candida isolates via inhibition multidrug efflux and itself effluxed Yor1. As...
Invasive fungal infections caused by the pathogen Candida albicans have transitioned from a rare curiosity to major cause of human mortality. This is in part due emergence resistance limited number antifungals available treat infections. Azoles function targeting biosynthesis ergosterol, key component cell membrane. Loss-of-function mutations ergosterol biosynthetic gene ERG3 mitigate azole toxicity and enable that depends upon stress responses. Here, we performed genome-wide synthetic...
Abstract To remodel their hosts and escape immune defenses, many pathogens rely on large arsenals of proteins (effectors) that are delivered to the host cell using dedicated translocation machinery. Effectors hold significant insight into biology both encode for them pathways they manipulate. One most powerful systems tools studying effectors is model organism, Saccharomyces cerevisiae . For pathogens, heterologous expression in yeast growth inhibitory at a frequency much higher than...
Abstract To remodel their hosts and escape immune defenses, many pathogens rely on large arsenals of proteins (effectors) that are delivered to the host cell using dedicated translocation machinery. Effectors hold significant insight into biology both encode them pathways they manipulate. One most powerful systems tools for studying effectors is model organism, Saccharomyces cerevisiae. For pathogens, heterologous expression in yeast growth inhibitory at a frequency much higher than...
uses over 300 translocated effector proteins to rewire host cells during infection and create a replicative niche for intracellular growth. To date, several studies have identified
Legionellosis was diagnosed in an immunocompromised 3-year-old girl Canada. We traced the source of bacterium through co-culture with ameba collected from a hot tub her home. identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm environmental clinical isolates were common origin.
Abstract Legionella pneumophila uses over 300 translocated effector proteins to rewire host cells during infection and create a replicative niche for intracellular growth. To date, several studies have identified L. effectors that indirectly directly regulate the activity of other effectors, providing an additional layer regulatory complexity. Amongst these are “metaeffectors” – special class once inside host. A defining feature metaeffectors is direct, physical interaction with target...
The eukaryotic CCR4-NOT deadenylase complex is a highly conserved regulator of mRNA metabolism that influences the expression complete transcriptome, representing prime target for generalist bacterial pathogen. We show translocated effector protein, PieF (Lpg1972)
ABSTRACT The eukaryotic CCR4-NOT deadenylase complex is a highly conserved regulator of mRNA metabolism that influences the expression complete transcriptome, representing prime target for generalist bacterial pathogen. We show translocated effector protein, PieF (Lpg1972) L. pneumophila Str. Philadelphia-1, interacts specifically with CNOT7/8 nuclease module CCR4-NOT, dissociation constant in low nanomolar range. inhibits catalytic subunit CNOT7 stoichiometric, dose-dependent manner vitro ....
Presentation of poster 2144B at TAGC 2020 Online. File is a PDF the (EnsmingerTAGC2020.pdf)