A5027094411- Dysphagia Assessment and Management
- Head and Neck Cancer Studies
- Tracheal and airway disorders
- Voice and Speech Disorders
- Head and Neck Surgical Oncology
- Ear and Head Tumors
- Reconstructive Surgery and Microvascular Techniques
- Salivary Gland Tumors Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Airway Management and Intubation Techniques
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Esophageal and GI Pathology
- Cancer Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Lymphoma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Facial Nerve Paralysis Treatment and Research
- Bone Tumor Diagnosis and Treatments
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience of respiration and sleep
- Oral and Maxillofacial Pathology
- Ear Surgery and Otitis Media
Yale University
2012-2021
Yale Cancer Center
1993-2020
National and Kapodistrian University of Athens
2010-2018
Evangelismos Hospital
2018
National Centre of Scientific Research "Demokritos"
2018
College Station Medical Center
2016-2017
Triological Society
2016-2017
Okayama Prefecture
2017
Nagano Prefecture Forestry Research Center
2016
Charlottesville Medical Research
2016
Purpose We sought to determine the prevalence of biologically relevant human papillomavirus (HPV) in oropharyngeal squamous cell carcinoma (OSCC). Retinoblastoma (Rb) downregulation by HPV E7 results p16 upregulation. hypothesized that overexpression OSCC defines HPV-induced tumors with favorable prognosis. Methods Using real-time polymerase chain reaction for HPV16, we determined HPV16 viral load a cohort 79 OSCCs annotated long-term patient follow-up. A tissue microarray including these...
Programmed death-ligand 1 (PD-L1; also known as CD274 or B7-H1) expression represents a mechanism of immune escape for cancer. Our purpose was to characterize tumor PD-L1 and associated T-cell infiltration in primary laryngeal squamous cell carcinomas (SCC).A well-annotated cohort 260 operable SCCs [formalin-fixed paraffin-embedded (FFPE) specimens] morphologically characterized stromal tumor-infiltrating lymphocytes (TIL), on hematoxylin/eosin-stained whole sections mRNA by qRT-PCR FFPE...
Successful application of programmed death 1 (PD1) checkpoint inhibitors in the clinic may ultimately benefit from appropriate patient selection based upon predictive biomarkers. Molecular characterization circulating tumor cells (CTC) is crucial for investigation molecular-targeted therapies while biomarkers response to PD1 are lacking. We sought assess whether overexpression PD-L1 CTCs could be detected at baseline and different timepoints during treatment a prospective cohort head neck...
In order to measure tinnitus induced by sodium salicylate injections, 84 pigmented rats, distributed among 14 groups in five experiments, were used a conditioned suppression paradigm. Experiment 1, all trained with stimulus (CS) consisting of the offset continuous background noise. One group began injections before Pavlovian training, second started after and control received daily saline injections. Resistance extinction was profound when but minimal initiated which suggests that...
Abstract Background: Several lines of evidence support the epidermal growth factor receptor (EGFR) as a molecular target for therapy in head and neck squamous cell carcinomas (HNSCC). Determination tumor EGFR levels by conventional immunohistochemistry has not always predicted antitumor efficacy. Quantitative assays may provide more accurate assessment level tumor, which thus reliable prognostic predictive information. We studied value quantitative oropharyngeal cancers treated with...
The E6 and E7 genes of human papillomavirus type 16 (HPV16) encode oncoproteins that bind degrade p53 retinoblastoma (pRb) tumor suppressors, respectively. We examined the effects repressing oncogene expression on transformed phenotype HPV16-positive oropharyngeal cancer cell lines. Human squamous 147T 090 (harboring integrated HPV16 DNA) 040T (HPV DNA–negative) cells were infected with retroviruses expressed a short hairpin RNA (shRNA) targeting or scrambled-sequence control shRNA. Flow...
PURPOSE: Vascular Endothelial Growth Factor (VEGF) promotes angiogenesis in many different tumor types. VEGF levels may affect growth, metastatic potential, and response to radiotherapy. This study assesses the prognostic value of protein a cohort patients with oral oropharyngeal squamous cell carcinomas. The relationships between clinical outcome covariables tumor-node-metastasis stage, disease stage (I IV), grade, margin status, race, sex, age were also determined. PATIENTS AND METHODS:...
Abstract Lyme disease is a multisystemic illness caused by tick‐borne spirochete. Once considered unique to the Connecticut coastline, thousands of cases are now documented throughout United States, northern Europe, and Australia. Unilateral bilateral facial paralysis may occur in up 11% patients with disease. This paper reviews clinical course, distinguishing features, outcome 124 such palsies 101 seen between 1975 1984. The 99.2% spontaneous recovery rate demonstrates unequivocally...
The magnetic resonance (MR) and computed tomographic (CT) images of 53 patients with sinonasal tumors were analyzed compared for accuracy in tumor mapping. the findings confirmed by means either surgery or biopsy. MR 60 inflammatory disease also studied, at surgery. Forty-seven additional examined showing histologic characteristics similar to those found group but occurring elsewhere head neck, excluding orbit. This study concludes that nearly 95% have an intermediate T2 signal, while only...
Abstract Subglottic stenosis carries devastating consequences. Its pathogenesis, and therefore prevention, have thus far eluded precise definition. The following data suggest that tracheostomy results in a contaminated wound, secondarily infecting larynx which may been injured by prior intubation, fracture, or surgery. interpretation of these is based upon the tested assumption infection prolongs healing tissue predisposes to scar stricture formation. Therefore, ability control stomal...
Changes in spontaneous neuronal activity of the inferior colliculus albino guinea pigs before and after administration sodium salicylate were analyzed. Animals anesthetized with pentobarbital, two microelectrodes separated by a few hundred microns driven through colliculus. After collecting sufficiently large sample cells, (450 mg/kg) was injected i.p. recordings again made 2 h injection. Comparison recorded revealed highly statistically significant differences (p<0.001). salicylate,...
Abstract The clinical observation of aspiration following prolonged tracheostomy prompted a neurophysiologic investigation the glottic closure reflex in dogs longitudinally evaluated after permanent tracheostomy. data support significant alterations central organization protective heretofore considered phylogenetically primitive and therefore physiologically stable over wide ranges functional demand. indicate that chronic upper airway bypass results in: 1. increased threshold evoked adductor...
• The function of the epiglottis in adult man is unclear. However, during early infancy epiglottic cartilage appears to play an important role separating upper respiratory tract from digestive tract. This separation accomplished by approximation palate, providing a contiuous airway nose through larynx into trachea. structural arrangement, however, uniquely lost postnatal development. Maturational descent epiglottis, found occur between 4 and 6 months age, verified cineradiography. change,...
Objective Establish the feasibility of a predictive validity study in sinus surgery simulation training and demonstrate effectiveness Endoscopic Sinus Surgery Simulator (ES3) as device. Study Design Prospective, multi‐institutional controlled trial. Setting Four tertiary academic centers with accredited otolaryngology‐head neck residency programs. Subjects Twelve ES3‐trained novice residents were compared 13 control residents. Methods assessed on performance basic tasks. Their first vivo...
Abstract Objectives: To determine the feasibility of sentinel node radiolocalization in stage N0 head and neck squamous cell carcinoma to gain insight as whether could be prognostic regional micrometastatic disease. Study Design: A prospective report on application eight patients with region. Methods: For each patient a peritumoral submucosal injection filtered technetium ( 99m Tc) prepared sulfur colloid was performed immediately following intubation. After at least 30 minutes, focal areas...
Functional inactivation of p16 is an early and frequent event in head neck squamous cell cancers. In this study, we sought to determine whether expression prognostic importance oropharyngeal carcinoma.p16 protein was evaluated by immunohistochemistry a tissue microarray composed 123 cancers with mean patient follow-up time 33 months.p16 overexpression associated more advanced Tumor-Node-Metastasis stage higher histologic grade. Despite association unfavorable features, decreased 5-year local...
Abstract The reported mortality (40%) and neurologic morbidity (25%) rates for carotid rupture remain unacceptably high. This study was conducted to assess the impact of endovascular detachable balloon occlusion changing characteristics in head neck surgery. Between January 1, 1988, June 30, 1994, 18 ruptures were identified 15 patients. Etiologic factors included radical surgery, radiation therapy, wound complications, recurrent or persistent carcinoma. In instances rupture, patients...
Computed tomography of temporal bone pneumatization: 1. Normal pattern and morphologyC Virapongse, M Sarwar, S Bhimani, C Sasaki R ShapiroAudio Available | Share
PURPOSE Two consecutive randomized trials were run at our institution using the bioreductive alkylating agent mitomycin as an adjunct to radiation therapy in effort improve outcome patients with squamous cell carcinoma of head and neck. METHODS Between 1980 1992, two (trial 1) dicumarol 2) neck conducted institution. The stratified by intent therapy, extent disease, primary tumor site. Within each strata, receive or without mitomycin/dicumarol 2). RESULTS A total 203 enrolled onto both...