A5058396834- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Health and Disease Prevention
- RNA Interference and Gene Delivery
- Cerebral Palsy and Movement Disorders
- Balance, Gait, and Falls Prevention
- Advanced biosensing and bioanalysis techniques
- Muscle activation and electromyography studies
- Intracranial Aneurysms: Treatment and Complications
- Acute Ischemic Stroke Management
- Stroke Rehabilitation and Recovery
- Venous Thromboembolism Diagnosis and Management
- Diagnosis and Treatment of Venous Diseases
- DNA and Nucleic Acid Chemistry
- Transcranial Magnetic Stimulation Studies
- Neurobiology of Language and Bilingualism
- Dermatologic Treatments and Research
- Genetic Neurodegenerative Diseases
- Medical Imaging and Analysis
- Central Venous Catheters and Hemodialysis
- Virus-based gene therapy research
- Musculoskeletal pain and rehabilitation
- Oropharyngeal Anatomy and Pathologies
- Microfluidic and Bio-sensing Technologies
- Sports Performance and Training
- Pain Mechanisms and Treatments
IRCCS Istituto Auxologico Italiano
2017-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2017-2022
University of Milan
2021
GTx (United States)
2020
Politecnico di Milano
2011-2019
National Interuniversity Consortium of Materials Science and Technology
2018-2019
Mylan (Switzerland)
2014-2015
Curculio Institute
2015
Milano University Press
2014
BackgroundAmong asymptomatic patients with severe carotid artery stenosis but no recent stroke or transient cerebral ischaemia, either stenting (CAS) endarterectomy (CEA) can restore patency and reduce long-term risks. However, from national registry data, each option causes about 1% procedural risk of disabling death. Comparison their protective effects requires large-scale randomised evidence.MethodsACST-2 is an international multicentre trial CAS versus CEA among thought to require...
The development of multifunctional vectors for efficient and safe gene delivery is one the major challenges scientists working in therapy field. In this context, we have designed a novel type aminoglycoside-rich dendrimers with defined structure based on polyamidoamine (PAMAM) order to develop efficient, nontoxic vehicles. Three different conjugates, i.e., PAMAM G4-neamine, -paromomycin, -neomycin, were synthesized characterized by nuclear magnetic resonance (NMR) MALDI analysis. conjugates...
Background Successful non-viral gene delivery currently requires compromises to achieve useful transfection levels while minimizing toxicity. Despite high molecular weight (MW) branched polyethylenimine (bPEI) is considered the gold standard polymeric transfectant, it suffers from cytotoxicity. Inversely, its low MW counterpart less toxic and effective in transfection. Moreover, chitosan a highly biocompatible biodegradable polymer but characterized by very efficiency. In this scenario,...
Commercially sourced cationic polymers for gene delivery are thoroughly characterized, compared and optimized.
The decoration of polyamidoamine (PAMAM) dendrimers with some functional moieties holds promises for improving the gene-delivery behavior resulting polyplexes. Herein, we reported synthesis and development non-viral gene vectors built on an array PAMAMs, spanning generation 2 (G2), G4, G7, specifically tethered multivalent neomycin (Neo) or guanidinoneomycin (GNeo). Following a thorough chemical characterization by means NMR MALDI analyses, ability PAMAM–(G)Neo conjugates to serve as...
Atherosclerosis is a systemic disease that leads to accumulation of deposits, known as atherosclerotic plaques, within the walls carotids. In particular, three types plaque can be distinguished: soft, fibrous, and calcific. Most computational studies who investigated interplay between blood flow on patient-specific geometries used nonstandard medical images directly delineate segment its components. However, these techniques are not so widely available in clinical practice. this context, aim...
Abstract Covalent attachment of a phenolic antioxidant analogue α‐tocopherol to graphite‐coated magnetic cobalt nanoparticles (CoNPs) provided novel magnetically responsive capable preventing the autoxidation organic materials and showing reduced toxicity toward human cells.
Purpose Endovascular ablation of the great saphenous vein has been proposed as a less invasive alternative to conventional ligation and stripping varicose veins. Outcomes patients treated with radiofrequency ClosureFast™ system over an eight-year period from single-center were evaluated. Methods Three-year follow-up data included duplex ultrasound scan, complication rate, questionnaires assess patients' QOL, level pain, days off work. Results A total 1080 consecutive (49.5 ± 18.6 years, 72%...
Balance, i.e., the ability not to fall, is often poor in neurological patients and this impairment increases their risk of falling. The Mini-Balance Evaluation System Test (Mini-BESTest), a rating scale, Timed Up Go (TUG) test, gait measures are commonly used quantify balance. This study assesses criterion validity these as balance measures.The probability being faller within nine months was criterion. Mini-BESTest, TUG (instrumented with inertial sensors), walking test were administered...
Polyplexes are nanoparticles formed by the self-assembly of DNA/RNA and cationic polymers specifically designed to deliver exogenous genetic material cells a process called transfection. There is general consensus that subtle balance between sufficient extracellular protection intracellular release nucleic acids key factor for successful gene delivery. Therefore, there strong need develop suitable tools techniques enabling monitoring stability polyplexes in biological environment they face...
In hemiplegic gait the paretic lower limb provides less muscle power and shows a briefer stance compared with unaffected limb. Yet, longer higher can be obtained from if speed is increased. This supports existence of ‘learned non-use’ phenomenon, similar to that underlying some asymmetric impairments motion eyes upper limbs. Crouch (CG) (bent-hip bent-knee, about 30° minimum knee flexion) might an effective form ‘forced-use’ treatment It not known whether it also stimulates more symmetric...
Neck proprioception is commonly assessed with head repositioning tests. In such a test, an operator rotates the of blindfolded individual to target position. After returning rest position, participant actively repositions target. Joint Position Error (JPE) angular difference between angle (however oriented in 3D space) and reached positions (the smaller difference, better proprioception). This study aimed validate head-to-target (HTT) test using optoelectronic system for also measuring...
Walking on a split-belt treadmill (each of the two belts running at different speed) has been proposed as an experimental paradigm to investigate flexibility neural control gait and form therapeutic exercise. However, scarcity dynamic investigations challenges validity available findings. The aim present study was asymmetries lower limbs healthy adults during adaptation treadmill. Ten walked mounted force sensors, with either same speed ('tied' condition) or speeds ('split' condition, 0.4...
Microfluidic platform for the high-throughput screening of gene delivery vectors and optimization transfection protocols at a single cell level.
Gait analysis (GA) typically includes surface electromyographic (sEMG) recording from several lower limb muscles, optoelectronic measurement of joint rotations, and force recordings ground-based platforms. From the latter two variables, muscle power acting on joints can be estimated. Recently, gait a split-belt treadmill (GAFT) was validated for study adult walking. It showed high reliability spatiotemporal, kinematic, dynamic, sEMG parameters, matching those obtainable with GA basis ground...
Twenty-six months after a left hemispheric ischemic stroke an aphasic patient showed significant improvement in verbal fluency following ten daily sessions of inhibitory 1 Hz repetitive transcranial magnetic stimulation over the right cortex homologous to Broca's area.No was observed for other linguistic functions or executive ones. Results confirm segregation neural circuitries subtending phonemic and semantic suggest selective usefulness treatment.
Few studies have investigated the kinematic aspects of body centre mass motion, that is, its three-dimensional path during strides and their changes with child development. This study aimed to describe in children while walking order disentangle effect age from absolute forward speed size define preliminary pediatric normative values. The was compared across healthy 5-6- years (n = 6), 7-8 9-10 5), 11-13 5) adults (23-48 years, n 6). Participants walked on a force-sensing treadmill at...
This single centre study investigates the influence of diabetes mellitus on outcomes following carotid artery endarterectomy or stenting.In total, 752 revascularizations (58.2% stenting and 41.8% endarterectomy) were performed in 221 (29.4%) patients with 532 (70.6%) without diabetes. The death, disabling non-disabling stroke, transient ischaemic attack restenosis within 36 months after procedure.Patients had higher periprocedural risk any stroke death (3.6% vs 0.6% no diabetes; p < 0.05),...
Since bacterial pollution is more troublesome than other nonbiologic air pollutants, the need to control airborne micro-organisms has led renewed interest in filter media for filtration indoor environments. Although mechanical of aerosols by HEPA systems most common method particle removal, these filters characterized high efficiency usually reveal a higher drop pressure and noise are very expensive. On this basis, we aimed develop novel, effective removal bacteria from confined...
Abstract Aims The metabolic syndrome (MetS) is composed of a cluster related cardiovascular risk factors. aim the present study was to determine how MetS contributes short‐ (30‐day) and long‐term complications restenosis after carotid endarterectomy (CEA) or stenting (CAS). Methods A consecutive cohort 752 patients undergoing CEA (n = 314) CAS 438) in single institution examined, which 296 (39.4%) were identified as having MetS. All followed‐up with duplex ultrasound scan supraaortic vessels...
During walking, falling is most likely to occur towards the side of supporting lower limb during single stance. Timely lateral redirection centre mass (CoM) preceding no-return position necessary for balance. We analysed curvature peaks (the inverse radius curvature) three-dimensional path CoM entire stride. Twelve healthy adults walked on a force-sensorized treadmill at constant velocities from 0.4 1.2 m s−1, in 0.2 s−1 increments. The displacements CoM, muscular power sustaining motion...
The aim of this retrospective single-center study is to describe and analyze short-, mid-, long-term risk factors for great saphenous vein (GSV) recanalization after endovenous radiofrequency ablation (RFA).All consecutive patients with GSV incompetence varicose veins underwent RFA were enrolled between 2009 2018. Data on demographic, pre- postoperative color Doppler scan (CDUS) findings, perioperative complications, follow-up prospectively collected. Primary outcome was rate at 1 week RFA....
Although rare, central post-stroke pain remains one of the most refractory forms neuropathic pain. Repetitive transcranial magnetic stimulation (rTMS) has been reported to be effective in chronic cases. However, there are no data on effects acute and subacute phases after stroke. In this study, we present a case patient with thalamic stroke onset paresthesia who was responsive rTMS. After right stroke, 32-year-old woman presented drug-resistant left side body. There were motor or sensory...