A5047874356- Protein Degradation and Inhibitors
- Cytokine Signaling Pathways and Interactions
- Multiple Myeloma Research and Treatments
- HIV/AIDS drug development and treatment
- NF-κB Signaling Pathways
- Ubiquitin and proteasome pathways
- Immune Response and Inflammation
- interferon and immune responses
- Helicobacter pylori-related gastroenterology studies
- Histone Deacetylase Inhibitors Research
- Inflammatory Bowel Disease
- Viral Infections and Immunology Research
- Pediatric health and respiratory diseases
- Cancer Research and Treatments
- Immune cells in cancer
- Asthma and respiratory diseases
- Animal testing and alternatives
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- Multiple Sclerosis Research Studies
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Psoriasis: Treatment and Pathogenesis
- IL-33, ST2, and ILC Pathways
Natera (United States)
2024
Rostov State Medical University
2024
Ministry of Health of the Russian Federation
2024
Eurofins (United States)
2019-2022
University of California, Davis
2008-2018
DiscoveRx (United States)
2015-2018
Bayer (United States)
2008-2010
Universitair Ziekenhuis Leuven
2007
Amsterdam UMC Location University of Amsterdam
2007
Abstract The histone acetyltransferases CBP/p300 are involved in recurrent leukemia-associated chromosomal translocations and key regulators of cell growth. Therefore, efforts to generate inhibitors clinical value. We developed a specific potent acetyl-lysine competitive protein–protein interaction inhibitor, I-CBP112, that targets the bromodomains. Exposure human mouse leukemic lines I-CBP112 resulted substantially impaired colony formation induced cellular differentiation without...
Abstract Mucin over-production is one of the hallmarks chronic airway diseases such as obstructive pulmonary disease, asthma, and cystic fibrosis. NF-κB activation in epithelial cells has been shown to play a positive inflammatory role diseases; however, mucin gene expression unresolved. In this study, we have that proinflammatory cytokines, IL-1β IL-17A, both which utilize pathway, are potent inducers (MUC)5AC mRNA protein synthesis by well-differentiated primary normal human bronchial cell...
A major pathological feature of chronic airway diseases is the elevated expression gel-forming mucins. NF-κB activation in epithelial cells has been shown to play a proinflammatory role diseases; however, specific mucin gene not characterized. In this study, we show that cytokines, IL-1β and IL-17A, both which use pathway, are potent inducers MUC5B mRNA well differentiated primary normal human bronchial cell line, HBE1. induction by these cytokines was time- dose-dependent, attenuated small...
Granulocyte-macrophage colony-stimulating factor (GM-CSF) therapy is effective in treating some Crohn's disease (CD) patients and protects mice from colitis induced by dextran sulfate sodium (DSS) administration. However, its mechanisms of action remain elusive. We hypothesized that GM-CSF affects intestinal mucosal repair.DSS colitic were treated with daily pegylated or saline clinical, histological, inflammatory parameters kinetically evaluated. Further, the role bone marrow-derived cells...
Abstract In addition to antiviral effects, Type I interferons (IFN) have potent antiproliferative and immunomodulatory activities. Because of these properties IFNs been evaluated as therapeutics for the treatment a number human diseases, including cancer. Currently, shown be efficacious only select cancers. The reason this is unclear. Recent evidence has demonstrated that some cancer cell types seem defective in their ability respond IFN. It suggested defects IFN signaling one mechanism by...
Janus kinase-signal transducers and activators of transcription (JAK-STAT) signaling is critical to multiple cellular processes, including survival, differentiation, proliferation. JAK-STAT dysregulation has been noted in inflammatory disorders, aberrant JAK2 pathway activation implicated myelofibrosis polycythemia vera. Moreover, 4 therapeutic inhibitors (ruxolitinib, fedratinib, momelotinib, pacritinib) have either approved or are advanced clinical development for myelofibrosis. Although...
The present study tested the hypothesis that murine (m)IFN-beta or mIFN-alpha(2) can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3). CVB3-inoculated male Balb/c mice exhibited signs of illness, including lethargy, progressive weight loss, death (10% on day 3 100% 8). Cardiac was high [4,277 +/- 1,009 plaque-forming units 25 5 copies CVB3/hypoxanthine guanine phosphoribosyl transferase 1 mRNA] 4. tissue severe inflammatory...
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS). Interferon-β (IFN-β) therapy for MS hypothesized to cause short-term and long-term changes in gene expression that shift inflammation from Th1 Th2. In vivo induction define kinetics response IFN-β a large cohort patients described. Differential peripheral blood mononuclear cells (PBMCs) obtained relapsing-remitting (RRMS) was assessed using high content microarrays. Rapid onset appeared within 4 h...
Ranges of absorbency in grams 1Corresponding term 6 and under Light absorbency.6 to 9Regular absorbency.
Type I interferons (IFNs) are cytokines that play a central role in mediating antiviral, antiproliferative, and immunomodulatory activities virtually all cells. These entirely dependent on the interaction of IFNs with their particular cell surface receptor. In this report, we identify two specific tyrosine residues located within cytoplasmic domain IFNAR2c obligatory for IFN-dependent signaling. Various mutants were expressed human lung fibroscarcoma line lacking (U5A). Stable clones...
We present evidence of a link between interferonβ-1b (IFN-β) and G-protein signaling by demonstrating that IFN-β can induce the expression negative regulator 1 (RGS1). RGS1 reduces activation immune cell migration interacting with heterotrimeric G-proteins enhancing their intrinsic GTPase activity. In this study, treatment resulted in induction peripheral blood mononuclear cells (PBMCs), monocytes, T cells, B cells. Induction was concentration dependent observed at both RNA protein level....
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent environmental toxicant. Epidemiological studies have associated TCDD exposure with the development of chronic obstructive pulmonary disease, which manifested by mucous/goblet cell hyperplasia. The purpose this research was to elucidate pathway/mechanisms that lead TCDD-induced gene expression in both primary normal human bronchial epithelial cells and an immortalized line, HBE1, under air-liquid interface conditions. induced...
In the context of human airway, interleukin-17A (IL-17A) signaling is associated with severe inflammation, as well protection against pathogenic infection, particularly at mucosal surfaces such airway. The intracellular molecule Act1 has been demonstrated to be an essential mediator IL-17A signaling. cytoplasm, it serves adaptor protein, binding both domain IL-17 receptor members canonical nuclear factor kappa B (NF-κB) pathway. It also enzymatic activity, and E3 ubiquitin ligase. airway...
Human type I interferons (IFNs) play an important role in the regulation of antiviral defense mechanisms, immunomodulatory activities, and growth control. Recent efforts have demonstrated importance IFNs activation ofsignal transducers activators transcription (STATs). The STAT1 STAT2 IFN-dependent JAK-STAT signaling is well established; however, STAT3 its by remains unclear. Understanding increasing interest because recent studies that may a cancer. Studies revealed constitutively active...
Identifying pan-tumor biomarkers that predict responses to immune checkpoint inhibitors (ICI) is critically needed. In the AMADEUS clinical trial (NCT03651271), patients with various advanced solid tumors were assessed for changes in intratumoral CD8 percentages and their response ICI. Patients grouped based on tumoral levels: those <15% (CD8-low) received nivolumab (anti-PD-1) plus ipilimumab (anti-CTLA4) ≥15% (CD8-high) monotherapy. 79 (72 CD8-low 7 CD8-high) treated. The disease...
Summary Cholera toxin (CT) is a bacterial component that increases intracellular cAMP levels in host cells and suppresses T‐cell activation. Recently, CT was reported to induce T helper type 17‐skewing dendritic activate interleukin‐17A (IL‐17A) production CD4 + through cAMP‐dependent pathway. However, the underlying mechanism by which regulates IL‐17A not completely defined. In this study, we took advantage of small molecule protein kinase A (PKA) inhibitor (H89) different analogues:...
Over the past two decades, much evidence has accumulated that confirms crucial role of alternative splicing in process tumorigenesis. A more detailed study mechanisms revealed targeting central for atypical cells could be a potential new approach treatment malignant neoplasms. Firstly, specific protein isoforms are formed as result and involved tumorigenesis can potentially act target Second, high rates cell proliferation presumably make tumor highly dependent on functional spliceosome,...
Defined content of fatty acids in lipids cat stem cells derived from fetal primary material. Fetal (FSCs) treated by culturing material the CO2 incubator containing 5% CO2, at a temperature 37°С DMEM medium with addition 15 – 20% bulls serum and 1% antibiotic–antimycotic. When confluent monolayer reached 70 80%, are removed culture dishes held subcultivation to reduce heterogeneity culture. The resulting tested for acid gas–liquid chromatography.Determination conducted under SOST ISO...