A5021846918- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Neurobiology and Insect Physiology Research
- Advanced Chemical Sensor Technologies
- Neuroscience and Neuropharmacology Research
- Cell Image Analysis Techniques
- Advanced Memory and Neural Computing
- Biochemical Analysis and Sensing Techniques
- Memory and Neural Mechanisms
- Medical Image Segmentation Techniques
- Image Retrieval and Classification Techniques
- Sleep and Wakefulness Research
- Photoreceptor and optogenetics research
- Remote-Sensing Image Classification
- Ion channel regulation and function
- CCD and CMOS Imaging Sensors
- Insect Pheromone Research and Control
- Spectroscopy Techniques in Biomedical and Chemical Research
- Image Processing Techniques and Applications
- Adipose Tissue and Metabolism
- EEG and Brain-Computer Interfaces
- Computational Drug Discovery Methods
- Essential Oils and Antimicrobial Activity
- bioluminescence and chemiluminescence research
The Francis Crick Institute
2017-2025
Hungarian Academy of Sciences
2006-2024
Czech Academy of Sciences, Institute of Experimental Medicine
2023-2024
HUN-REN Institute of Experimental Medicine
2006-2023
University College London
2017-2022
Max Planck Institute for Medical Research
2012-2022
Heidelberg University
2019-2022
Integrating camera chips into neural recording arrays provides a massively parallel brain-machine interface.
The olfactory bulb (OB) is the first site of synaptic odor information processing, yet a wealth contextual and learned has been described in its activity. To investigate mechanistic basis modulation, we use whole-cell recordings to measure responses across rapid learning episodes identified mitral/tufted cells (MTCs). Across these episodes, diverse response changes occur already during sniff cycle. Motivated mice develop active sniffing strategies that robustly correspond changes, resulting...
In cerebellar Purkinje cell dendrites, heterosynaptic calcium signaling induced by the proximal climbing fiber (CF) input controls plasticity at distal parallel (PF) synapses. The substrate and regulation of this long-range dendritic are poorly understood. Using high-speed imaging, we examine role active conductances. Under basal conditions, CF stimulation evokes T-type displaying sharp proximodistal decrement. Combined mGluR1 receptor activation depolarization, two activity-dependent...
Potassium channels comprise the most diverse family of ion channels. In nerve cells, their critical roles in synaptic integration and output generation have been demonstrated. Here, we provide evidence for a distribution that predicts novel role K + CNS. Our experiments revealed highly selective clustering Kv4.3 A-type channel subunits at specialized junctions between climbing fibers cerebellar GABAergic interneurons. High-resolution ultrastructural immunohistochemical demonstrated these are...
<h3>Abstract</h3> In awake mice, sniffing behavior is subject to complex contextual modulation. It has been hypothesized that variance in inhalation dynamics alters odor concentration profiles the naris despite a constant environmental concentration. Using whole-cell recordings olfactory bulb of we directly demonstrate rapid mimics effect increase at level both mitral and tufted cell (MTC) firing rate responses temporal responses. Paradoxically, find mice are capable discriminating fine...
A core organizing principle of the vertebrate brain is its symmetry along multiple axes. However, structure these axes, and precision with which neurons, circuit modules, regions align to them, remain poorly understood. Here, we used 3D spatial transcriptomics reconstruct anatomical molecular organization mouse olfactory bulb. We mapped positions nearly one thousand molecularly distinct glomeruli, structural functional units odor processing, revealing highly symmetric across hemispheres....
Information is routed between brain areas via parallel streams. Neurons may share common inputs yet convey distinct information to different downstream targets. Here, we leverage the anatomical organisation of mouse olfactory bulb (OB), where dozens projection neurons (mitral and tufted cells, M/TCs) affiliate with a single input unit, glomerulus. To link functional properties M/TCs their glomerular association at scale, combine in vivo two-photon (2P) imaging synchrotron μCT analysis...
Although topographical maps organize many peripheral sensory systems, it remains unclear whether olfactory neurons (OSNs) choose which of the ~1100 odor receptors (ORs) to express based upon their spatial location in epithelium (OE) or instead ORs are scattered randomly. Here we reveal that each OR is expressed at a precise mean position along OE dorsoventral axis, thereby instantiating receptor map. This patterning reflects differential use, by precursors and mature OSNs, coherent gene...
Abstract Alcohol intake associates with overeating in humans. This is a clinical concern, but its causes are puzzling, because alcohol (ethanol) calorie-dense nutrient, and calorie usually suppresses brain appetite signals. The biological factors necessary for ethanol-induced remain unclear, societal have been proposed. Here we show that core elements of the brain’s feeding circuits—the hypothalamic Agrp neurons normally activated by starvation evoke intense hunger—display electrical...
Abstract Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying totality cells belonging such neuronal modules, “inputs” and “outputs,” remains a major challenge. Here, we present development nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation substantial volume tissue. Combining finite element modeling focused ion beam milling, NEMs permit substantially higher...
Juxtaglomerular cells (JGCs) of the olfactory bulb (OB) glomerular layer (GL) play a fundamental role in information processing. Their variability morphology, physiology, and connectivity suggests distinct functions. The quantitative understanding population-wise morphological physiological properties comprehensive classification based on parameters, however, is still lacking, impeding analysis microcircuits. Here, we provide multivariate clustering 95 <i>in vitro</i> sampled from GL mouse...
Mammalian brains consist of 10s millions to 100s billions neurons operating at millisecond time scales, which current recording techniques only capture a tiny fraction. Recording capable sampling neural activity high spatiotemporal resolution have been difficult scale. The most intensively studied mammalian neuronal networks, such as the neocortex, show layered architecture, where optimal technology samples densely over large areas. However, need for application-specific designs well...
Abstract Multi-channel electrical recordings of neural activity in the brain is an increasingly powerful method revealing new aspects communication, computation, and prosthetics. However, while planar silicon-based CMOS devices conventional electronics scale rapidly, interface have not kept pace. Here, we present a strategy to chips with three-dimensional microwire arrays, providing link between rapidly-developing high density interfaces. The system consists bundle microwires mated...
Abstract Theoretical and functional studies predicted a highly non‐uniform distribution of voltage‐gated ion channels on the neuronal surface. This was confirmed by recent immunolocalization experiments for Na + , Ca 2+ hyperpolarization activated mixed cation K channels. These also indicated that some were clustered in synaptic or non‐synaptic membrane specializations. Here we analysed subcellular Kv4.2 Kv4.3 subunits rat main olfactory bulb at high resolution to address whether clustering...
During wakefulness, the VTA represents valence of experiences and mediates affective response to outside world. Recent work revealed that two major populations - dopamine GABA neurons are highly active during REM sleep less NREM sleep. Using long-term cell type brain state-specific recordings, machine learning, optogenetics, we examined role sleep-activity these plays in subsequent awake behavior. We found activity (but not REM) correlated with exploratory features next day's Disrupting...
Summary Mammalian brains consist of 10s millions to 100s billions neurons operating at millisecond time scales, which current recording techniques only capture a tiny fraction. Recording capable sampling neural activity such temporal resolution have been difficult scale: The most intensively studied mammalian neuronal networks, as the neocortex, show layered architecture, where optimal technology samples densely over large areas. However, need for application-specific designs well mismatch...
The olfactory bulb (OB) is the very first site of odor information processing, yet a wealth contextual and learned has been described in its activity. To investigate mechanistic basis modulation, we use whole-cell recordings to measure responses across rapid (<30 min) learning episodes identified mitral/tufted cells (MTCs). Across these episodes, found that diverse response changes occur already during sniff cycle. Motivated mice develop active sniffing strategies learning, it this change...
Summary For stable perception of odor intensity, there must exist a neural correlate that is invariant across other parameters, such as the highly variable sniff cycle. Previous hypotheses suggest variance in inhalation dynamics alters concentration profiles naris despite constant environmental concentration. Using whole cell recordings olfactory bulb awake mice, we directly demonstrate rapid sniffing mimics effect increase at level both mitral and tufted (MTC) firing rate responses temporal...
Unsupervised segmentation in biological and non-biological images is only partially resolved. Segmentation either requires arbitrary thresholds or large teaching datasets. Here we propose a spatial autocorrelation method based on Local Moran’s I coefficient to differentiate signal, background noise any type of image. The method, originally described for geoinformatics, does not require predefined intensity threshold algorithm image allows quantitative comparison samples obtained different...