A5067884120- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Axon Guidance and Neuronal Signaling
- Memory and Neural Mechanisms
- Olfactory and Sensory Function Studies
- Bone fractures and treatments
- Periodontal Regeneration and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Dental Research and COVID-19
- Dental materials and restorations
- Crystallography and Radiation Phenomena
- Craniofacial Disorders and Treatments
- Electron and X-Ray Spectroscopy Techniques
- Neural dynamics and brain function
- dental development and anomalies
- Machine Learning in Materials Science
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Cleft Lip and Palate Research
- Facial Trauma and Fracture Management
The Francis Crick Institute
2018-2024
Biomedical Research Networking Center on Neurodegenerative Diseases
2010-2019
Universitat de Barcelona
1992-2019
Vall d'Hebron Institut de Recerca
2015-2016
Centro de Investigación Biomédica en Red
2015-2016
Instituto de Salud Carlos III
2010-2016
Barcelona Biomedical Research Park
2016
Institute for Research in Biomedicine
2010-2012
State University of New York
2007
Aarhus University
1995-2001
Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure cells and tissues through continuous depths at least 1 micrometer. A burgeoning grassroots community effort fast building profile revealing impact vEM technology in life sciences clinical research.
A substantial interest exists in developing substitute materials and human recombinant boneinducing factors to enhance bone regeneration both the craniofacial complex other parts of skeleton. persistent problem has been find an animal model that allows for comparison different osteopromotive materials. The purpose this investigation was 1) determine whether a 5-mm calvarial defect adult rats fulfilled requirements critical-size 2) discuss use assessing repair region. Bilateral full-thickness...
Reelin, an extracellular protein essential for neural migration and lamination, is also expressed in the adult brain. To unravel function of this forebrain, we generated transgenic mice that overexpress Reelin under control CaMKIIα promoter. Overexpression increased neurogenesis impaired positioning adult-generated neurons. In hippocampus, overexpression resulted increase synaptic contacts hypertrophy dendritic spines. Induction long-term potentiation (LTP) alert-behaving showed evokes a...
Abstract Lafora disease (LD) is caused by mutations in either the laforin or malin gene. The hallmark of accumulation polyglucosan inclusions called Bodies (LBs). Malin knockout (KO) mice present accumulations several brain areas, as do patients LD. These structures are abundant cerebellum and hippocampus. Here, we report a large increase glycogen synthase (GS) these mice, which enzyme accumulates LBs. Our study focused on hippocampus where, under physiological conditions, astrocytes...
Abstract Several members of the bone morphogenetic protein (BMP) and transforming growth factor-β (TGF-β) families are molecular regulators cartilage regeneration, although their actual roles combined interactions in skeletal repair poorly understood. The presence several forms suggests multiple functions vivo as well synergistic during both embryonic development regeneration postfetal life. Here we show for first time that recombinant human factor-β1 (TGF-β1) induces endochondral formation...
Despite the impact of schizophrenia and mood disorders, which in extreme cases can lead to death, recent decades have brought little progress development new treatments. Recent studies shown that Reelin, an extracellular protein is critical for neuronal development, reduced bipolar disorder patients. However, data on a causal or protective role Reelin psychiatric diseases scarce. In order study direct influence Reelin's levels behavior, we subjected two mouse lines, are either (Reelin...
Patients with Alzheimer's disease (AD) display altered functioning of cortical networks, including patterns synchronous activity and a serious deficit in cholinergic septohippocampal (SH) innervation. However, the mechanisms underlying these alterations implication GABAergic SH component AD are largely unknown. In addition, pathway (SHP) is believed to regulate hippocampal by controlling interneurons. Here we show, using well-characterized tracing experiments, that innervation SHP decreases...
Adult hippocampal neurogenesis is thought to be essential for learning and memory, has been implicated in the pathogenesis of several disorders. Although recent studies have identified key factors regulating neuroprogenitor proliferation adult hippocampus, mechanisms that control migration integration adult-born neurons into circuits are largely unknown. Reelin an extracellular matrix protein vital neuronal development. Activation cascade leads phosphorylation Disabled-1, adaptor required...
The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture samples calls for their reconstruction from serial sections. Here we show that focused ion beam/scanning (FIB/SEM) allows efficient, complete, automatic identified dendrites, including spines synapses, GFP/DAB-labeled neurons, a resolution comparable to TEM. We applied this technology analyze...
Understanding the function of biological tissues requires a coordinated study physiology and structure, exploring volumes that contain complete functional units at detail resolves relevant features. Here, we introduce an approach to address this challenge: Mouse brain tissue sections containing region where was recorded using in vivo 2-photon calcium imaging were stained, dehydrated, resin-embedded imaged with synchrotron X-ray computed tomography propagation-based phase contrast (SXRT)....
The extracellular protein Reelin has an important role in neurological diseases, including epilepsy, Alzheimer's disease and psychiatric targeting hippocampal circuits. Here we address the of development synaptic contacts adult-generated granule cells (GCs), a neuronal population that is crucial for learning memory implicated diseases. We found pathway controls shapes, sizes, types dendritic spines, complexity multisynaptic innervations degree perisynaptic astroglial ensheathment...
Abstract Objective: To analyze the long-term effect of mandibular bone as donor material in grafting alveolar process defect patients with unilateral cleft lip and palate (UCLP), compared iliac crest cancellous bone. Method: During a 7-year period, 101 UCLP were grafted, 57 cases 44 symphyseal The results an observation time more than 4 years analyzed respect to marginal level dental gingival condition grafted area. Complications recorded. Results: area was satisfactory both groups....
Guided bone regeneration is defined as controlled stimulation of new formation in a bony defect, either by osteogenesis, osteoinduction, or osteoconduction, re-establishing both structural and functional characteristics. Bony defects may be found result congenital anomalies, trauma, neoplasms, infectious conditions. Such conditions are often associated with severe esthetic problems. Corrective treatment complicated limitations tissue adaptations. The aim the investigation was to compare...
Objective To analyze the long-term effect of mandibular bone as donor material in grafting alveolar process defect patients with unilateral cleft lip and palate (UCLP), compared iliac crest cancellous bone. Method During a 7-year period, 101 UCLP were grafted, 57 cases 44 symphyseal The results an observation time more than 4 years analyzed respect to marginal level dental gingival condition grafted area. Complications recorded. Results area was satisfactory both groups. Impaction cleft-side...
Reelin regulates neuronal positioning and synaptogenesis in the developing brain, adult brain plasticity. Here we used transgenic mice overexpressing (Reelin-OE mice) to perform a comprehensive dissection of effects this protein on structural biochemical features dendritic spines axon terminals hippocampus. Electron microscopy (EM) revealed both higher density synapses complexity pre- postsynaptic elements than WT mice. Dendritic had larger spine apparatuses, which correlated with...
Coherent X-ray microscopy is emerging as a transformative technology for neuronal imaging, with the potential to offer scalable solution reconstruction of neural circuits in millimeter sized tissue volumes. Specifically, holographic nanotomography (XNH) brings together outstanding capabilities terms contrast, spatial resolution and data acquisition speed. While recent XNH developments already enabled generating valuable datasets neurosciences, major challenge remained overcoming resolving...
Information is routed between brain areas via parallel streams. Neurons may share common inputs yet convey distinct information to different downstream targets. Here, we leverage the anatomical organisation of mouse olfactory bulb (OB), where dozens projection neurons (mitral and tufted cells, M/TCs) affiliate with a single input unit, glomerulus. To link functional properties M/TCs their glomerular association at scale, combine in vivo two-photon (2P) imaging synchrotron μCT analysis...
Abstract Dense microcircuit reconstruction techniques have begun to provide ultrafine insight into the architecture of small-scale networks. However, identifying totality cells belonging such neuronal modules, “inputs” and “outputs,” remains a major challenge. Here, we present development nanoengineered electroporation microelectrodes (NEMs) for comprehensive manipulation substantial volume tissue. Combining finite element modeling focused ion beam milling, NEMs permit substantially higher...
Bone healing plays an important role in orthognathic and craniofacial surgery. tissue repair regeneration are regulated by array of growth morphogenetic factors. Osteogenesis proceeds through a cascade molecular cellular events sequentially coordinated members both the bone protein transforming factor-(3 (TGF-β) families. The efficacy single application 2,5, or 10 μg recombinant human (rh) TGF-βl to promote 5-mm experimental cal-varial defects adult male rats was assessed histolog-ically...
Abstract The therapeutic utility of a single application recombinant human transforming growth factor-β (hTGF-β) has not been previously tested in large osseous wounds primates. Sixteen calvarial defects, 25 mm diameter, were prepared four adult male baboons (Papio ursinus). In each animal, three defects treated with increasing doses hTGF-β1 conjunction baboon insoluble collagenous bone matrix as carrier (5, 30, and 100 μg hTGF-β1/g matrix). fourth defect was implanted without control....
The purpose of this study was to compare the clinical performance a resin‐reinforced self‐cured glass ionomer cement standard composite resin in split mouth design, by using both systems for direct bonding orthodontic stainless steel brackets every patient. Forty eight patients (34 females and 14 males, which 29 were adults > 18 years age) with fixed appliances followed mean period 10 months (range 4–16 months). 864 evaluated: 404 bonded GC Fuji Ortho (GC Industrial, Tokyo, Japan) onto...