A5057607023- Aquaculture disease management and microbiota
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Zebrafish Biomedical Research Applications
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Microbial infections and disease research
- CRISPR and Genetic Engineering
- Invertebrate Immune Response Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Venomous Animal Envenomation and Studies
- Pancreatic function and diabetes
- Platelet Disorders and Treatments
- Herpesvirus Infections and Treatments
- Xenotransplantation and immune response
- Diabetes and associated disorders
- Epigenetics and DNA Methylation
- Animal Virus Infections Studies
- Veterinary Oncology Research
- RNA Interference and Gene Delivery
- Blood groups and transfusion
- Mesenchymal stem cell research
- Antimicrobial Peptides and Activities
Nihon University
2010-2024
Abstract Hematopoietic stem cells (HSCs) maintain the entire blood system throughout life and are utilized in therapeutic approaches for diseases. Prospective isolation of highly purified HSCs is crucial to understand molecular mechanisms underlying regulation HSCs. The zebrafish an elegant genetic model study hematopoiesis due its many unique advantages. It has not yet been possible, however, purify adult a lack specific HSC markers. Here we show enrichment by combination two HSC-related...
Polymorphisms of canine leukocyte antigen (DLA) class I (DLA-88 and DLA-12/88L) II (DLA-DRB1) genes are important for disease susceptibility studies, but information on the genetic diversity among dog breeds is still lacking. To better elucidate polymorphism between breeds, we genotyped DLA-88, DLA-12/88L, DLA-DRB1 loci using 829 dogs 59 in Japan. Genotyping by Sanger sequencing identified 89, 43, 61 alleles loci, respectively, a total 131 DLA-88–DLA-12/88L–DLA-DRB1 haplotypes...
Mammalian granulocyte colony-stimulating factor (G-CSF; CSF3) is a primary cytokine that promotes the development, mobilization, and activation of neutrophils their precursors. Teleosts have been reported to possess two paralogs as likely result teleost-wide whole genome duplication (WGD) event, but functional divergence G-CSF remains poorly understood. Common carp are an allotetraploid species owing additional WGD event in lineage here, we report on genomic synteny, sequence similarity,...
The major histocompatibility complex (MHC) is a highly polymorphic and duplicated genomic region that encodes transplantation immune regulatory molecules. Although it well-known particular MHC allelic polymorphisms haplotypes are genetically relate to immune-mediated diseases detailed information of the cat (Feline Leukocyte Antigen; FLA) genetic haplotypic structure diversity limited in comparison humans many other species. In this study, better understand degree types allele haplotype...
Idiopathic non-infectious meningoencephalomyelitis (NIME), which is thought to be an immune-mediated disease, a common inflammatory disease in dogs. Meningoencephalomyelitis of unknown origin (MUO), subgroup NIME, consists necrotizing meningoencephalitis (NME), leukoencephalitis, and granulomatous meningoencephalomyelitis. Recent studies have shown associations between development dog leukocyte antigen (DLA) class II genes NME Pugs NIME Greyhounds. This study focused on Chihuahuas, high...
Neutrophils are essential for innate immunity as the first line of defence. act phagocytic white blood cells to kill bacteria and other microorganisms. A strong respiratory burst neutrophils, dependent on reactive oxygen species, is produced during phagocytosis. Platelet-activating factor (PAF) a signalling molecule with several prominent roles in tissue injury, inflammation, platelet aggregation. However, detailed mechanisms intracellular pathways involved PAF-mediated neutrophil activation...
Porcine circovirus 3 (PCV3) was first reported in the United States 2016; this virus is considered to be involved diverse pathologies, such as multisystem inflammation, porcine dermatitis and nephropathy syndrome, reproductive disorders. However, successful isolation of PCV3 using cultured cells has been rare. In study, we aimed isolate primary bone marrow-derived cells. Mononuclear were isolated from femur bones clinically healthy pigs. These for 6–10 days post-seeding infected with...
イヌは小動物臨床における最も重要な伴侶動物の一種である。イヌの主要組織適合性複合体(Dog leukocyte antigen, DLA)遺伝子群の多型情報の整備は,疾患関連解析の実施や,造血幹細胞や体性幹細胞の他家移植による再生医療やがん免疫療法などを含む先端獣医療の発展に向けて必須である。これまでDLA class II遺伝子群については大規模な多型解析が実施されていたが,DLA I遺伝子群の多型解析は極めて少なく,多型性は不明であった。筆者らはこれまでに59犬種829頭の日本国内のイヌについてDLA多型解析を実施した。本稿では,筆者らが解明したDLA I遺伝子領域の新規構造多型やDLA I遺伝子群の多型性に加え,DLA Iおよびclass II遺伝子を含む犬種内および犬種間のDLAハプロタイプの多様性の特徴についての最新情報を概説する。
Transplantation medicine is used for the treatment of severe canine diseases, and dog leukocyte antigen (DLA) considered to be important in graft rejection. However, utility direct sequencing both DLA classes I II has not been assessed thoroughly. Eight healthy beagles with identified genes were divided into two sets four dogs, each including one donor three recipients skin transplantation. The following selected: a complete match, haploidentical mismatch gene donor. Full-thickness segments...