A5073141832- Zebrafish Biomedical Research Applications
- Bone Metabolism and Diseases
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- T-cell and B-cell Immunology
- Angiogenesis and VEGF in Cancer
- Bone health and osteoporosis research
- Circadian rhythm and melatonin
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Dietary Effects on Health
- Stress Responses and Cortisol
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Ion channel regulation and function
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Endometriosis Research and Treatment
- Aquaculture disease management and microbiota
- Electromagnetic Fields and Biological Effects
- Single-cell and spatial transcriptomics
- Bone Tissue Engineering Materials
- Birth, Development, and Health
- Morphological variations and asymmetry
- interferon and immune responses
Komatsu University
2022-2024
Kanazawa University
2019-2024
Ishikawa Prefectural University
2024
The University of Tokyo
2019
University of California, San Diego
2014
Abstract Differentiation of osteoclasts (OCs) from hematopoietic cells requires cellular interaction with osteoblasts (OBs). Due to the difficulty live-imaging in bone, however, and molecular mechanisms underlying intercellular communication involved OC differentiation are still elusive. Here, we develop a fracture healing model using scale trap:GFP ; osterix:mCherry transgenic zebrafish visualize between OCs OBs. Transplantation assays followed by flow cytometric analysis reveal that most...
Abstract Hematopoietic stem cells (HSCs) maintain the entire blood system throughout life and are utilized in therapeutic approaches for diseases. Prospective isolation of highly purified HSCs is crucial to understand molecular mechanisms underlying regulation HSCs. The zebrafish an elegant genetic model study hematopoiesis due its many unique advantages. It has not yet been possible, however, purify adult a lack specific HSC markers. Here we show enrichment by combination two HSC-related...
ABSTRACT In order to efficiently derive hematopoietic stem cells (HSCs) from pluripotent precursors, it is crucial understand how mesodermal acquire and endothelial identities: two divergent, but closely related, cell fates. Although Npas4 has been recently identified as a conserved master regulator of hemato-vascular development, the molecular mechanisms underlying fate divergence between vascular are still unclear. Here, we show in zebrafish that differentiation into regulated by...
Abstract The maintenance and proliferation of hematopoietic stem cells (HSCs) are tightly regulated by their niches in the bone marrow. analysis niche or stromal cell lines that can support HSCs has facilitated finding novel supporting factors for HSCs. Despite large efforts murine marrow; however, HSC expansion is still difficult ex vivo, highlighting need new approaches to elucidate molecular elements regulate zebrafish provides a unique model study as maintained kidney, allowing parallel...
Electromagnetic fields (EMFs) have received widespread attention as effective, noninvasive, and safe therapies across a range of clinical applications for bone disorders. However, due to the various frequencies devices, their effects on tissues/cells are vary, which has been bottleneck in understanding EMFs tissue. Here, we developed an vivo model system using zebrafish scales investigate extremely low-frequency (ELF-EMFs) fracture healing. Exposure 10 millitesla (mT) ELF-EMFs at 60 Hz...
The zebrafish is a unique model to understand hematopoietic niches as stem/progenitor cells are maintained in the kidney. However, little known about which cell types kidney play role niches. Here, we demonstrate that sinusoidal endothelium an essential and conserved niche component Histological analysis revealed runx1:mCherry+ were predominantly detected dorsolateral region of where sinusoids highly developed. Loss Junctional adhesion molecule 1a (Jam1a), expressed both endothelial cells,...
Electromagnetic fields (EMFs) noninvasively promote fracture healing, prevent osteoporosis, diaphyseal growth, enhance differentiation, and stimulate cell division. However, no good model systems for analyzing bone regeneration have been reported. In this study, we examined the in vivo of scales having osteoblasts osteoclasts using a new magnetic field generator exposing aquatic animals to EMFs at sine-wave frequency 60 Hz. Goldfish were put into fish-breeding space with developed exposed...
Exposure to microgravity during space flight has caused astronauts experience decreased bone density, resulting from resorption by osteoclastic activation. Activation of the nuclear factor-κB ligand (RANKL) RANKL-producing cells, a known osteoclastogenesis-promoting factor, seems be induced under microgravity. However, role cells not yet been demonstrated due lack suitable in vitro organ culture osteoblasts, osteoclasts, and osteocytes with intact matrix Previous reports that were detected...
Abstract In order to efficiently derive hematopoietic stem cells (HSCs) from pluripotent precursors, it is crucial understand how mesodermal acquire or endothelial identity due their close developmental connection. Although Npas4 has been recently identified as a conserved master regulator of hemato-vascular development, the molecular mechanisms underlying cell fate divergence between and vascular are still unclear. Here, we show in zebrafish that regulated by Junctional adhesion molecule 3b...
Melatonin has diverse effects, and been reported to promote bone formation in addition regulating the sleep–wake cycle. In present study, we investigated effects of melatonin on metabolism using ovariectomized (OVX) rats; a model postmenopausal osteoporosis. Here, focused differences when was subcutaneous injected at day or early night. The OVX rats were with once daily (0.8 8 mg/head) between 11:00 14:00 18:00 19:30 for night, respectively, six weeks. After completion injection, femur tibia...
Introduction: Genetic testing for inherited arrhythmias and discriminating pathogenic or benign variants from of unknown significance (VUS) is essential gene-based medicine. KCNQ1 a causative gene type 1 long QT syndrome (LQTS) familial atrial fibrillation (AF), approximately 30% the found in LQTS are classified as VUS. Aims: We studied role zebrafish cardiac arrhythmia model determining clinical variants. Methods Results: generated homozygous kcnq1 deletion ( del/del ) using CRISPR/Cas9...