A5087967810- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Neurobiology and Insect Physiology Research
- Cancer-related gene regulation
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Animal Genetics and Reproduction
- Retinal Development and Disorders
- Animal Behavior and Reproduction
- Plant Molecular Biology Research
Research Institute of Molecular Pathology
2015-2024
Vienna Biocenter
2018-2023
University of Cambridge
2011
The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to Pol C-terminal domain dismantles elongating from DNA vitro. We demonstrate that PCF11-mediated termination essential for vertebrate development. range genomic analyses, including mNET-seq, 3′ mRNA-seq, chromatin RNA-seq, ChIP-seq, reveals PCF11 enhances stimulates early genome-wide....
Identifying distinct anatomical structures within the brain and developing genetic tools to target them are fundamental steps for understanding function. We hypothesize that enhancer expression patterns can be used automatically identify functional units such as neuropils fiber tracts. two recent, genome-scale Drosophila GAL4 libraries associated confocal image datasets segment large regions into smaller subvolumes. Our results (available at https://strawlab.org/braincode) support this...
Abstract The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show both mouse and zebrafish DCST1 DCST2 are necessary sperm to fertilize egg, similar their orthologs SPE-42 SPE-49 C. elegans Sneaky D. melanogaster . Mouse Dcst1 Dcst2 single knockout (KO) able undergo acrosome reaction normal relocalization IZUMO1, an essential factor fusion, equatorial segment. While KO...
Significance We show that Bouncer’s homolog in mammals, SPACA4, is required for efficient fertilization mice. In contrast to fish, which Bouncer female fertility, SPACA4 expressed exclusively the sperm and male fertility. present an intriguing example of homologous proteins both play key roles reproduction yet diverged terms gene expression pattern mode action. Overall, our work identifies as important protein necessary zona pellucida penetration during mammalian fertilization. Since human...
Abstract Molecular compatibility between gametes is a prerequisite for successful fertilization. As long as sperm and egg can recognize bind each other via their surface proteins, gamete fusion may occur even members of separate species, resulting in hybrids that impact speciation. The membrane protein Bouncer confers species specificity to interactions medaka zebrafish, preventing cross-fertilization. Here, we leverage this uncover distinct amino acid residues N-glycosylation patterns...
Fertilization is a key process in all sexually reproducing species, yet the molecular mechanisms that underlie this event remain unclear. To date, only few proteins have been shown to be essential for sperm-egg binding and fusion mice, some are conserved across vertebrates. One of these conserved, testis-expressed factors SPACA6, its function has not investigated outside mammals. Here we show zebrafish spaca6 encodes sperm membrane protein which fertilization. Zebrafish knockout males...
ABSTRACT Fertilization, the fusion of sperm and egg, is essential for sexual reproduction. While several proteins have been demonstrated to be binding gametes in vertebrates, molecular mechanisms driving this key process are poorly understood. Here, we performed a protein interaction screen using AlphaFold-Multimer uncover protein-protein interactions fertilization. This resulted prediction trimeric complex composed fertilization factors Izumo1 Spaca6, Tmem81, previously not implicated We...
Abstract The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show both mouse and zebrafish DCST1 DCST2 are necessary sperm to fertilize egg, similar their orthologs SPE-42 SPE-49 C. elegans Sneaky D. melanogaster . Mouse Dcst1 Dcst2 single knockout (KO) spermatozoa able undergo acrosome reaction normal relocalization IZUMO1, an essential factor fusion, equatorial...
Abstract All sexually reproducing organisms depend on fertilization to survive as species. Despite the importance of fertilization, mechanisms that drive sperm-egg compatibility are poorly understood. In fish, egg protein Bouncer is necessary for and species-specific between medaka zebrafish. Here, we investigate whether generally in fish identify features mediating its medaka/zebrafish specificity. vitro experiments using zebrafish show not a general specificity factor. Instead, homologs...
Abstract We made use of two recent, large-scale Drosophila GAL4 libraries and associated confocal imaging datasets to automatically segment large brain regions into smaller putative functional units such as neuropils fiber tracts. The method we developed is based on the hypothesis that molecular identity can be used assign individual voxels biologically meaningful regions. Our results (available at https://strawlab.org/braincode ) are consistent with this because well-known anatomy, namely...
SUMMARY Fertilization is the fundamental process that initiates development of a new individual in all sexually reproducing species. Despite its importance, our understanding molecular players govern mammalian sperm-egg interaction incomplete, partly because many essential factors found non-mammalian species do not have obvious homologs. We recently identified Ly6/uPAR protein Bouncer as new, fertilization factor zebrafish (Herberg et al., 2018). Here, we show Bouncer’s homolog mammals,...
The pervasive nature of RNA polymerase II (Pol II) transcription requires efficient termination. A key player in this process is the cleavage and polyadenylation (CPA) factor PCF11, which directly binds to Pol C-terminal domain dismantles elongating from DNA vitro. We demonstrate that PCF11-mediated termination essential for vertebrate development. range genomic analyses, including: mNET-seq, 3' mRNA-seq, chromatin RNA-seq ChIP-seq, reveals PCF11 enhances stimulates early genome-wide....
Abstract Fertilization is a key process in all sexually reproducing species, yet the molecular mechanisms that underlie this event remain unclear. To date, only few proteins have been shown to be essential for sperm-egg binding and fusion mice, some are conserved across vertebrates. One of these conserved, testis-expressed factors SPACA6, its function has not investigated outside mammals. Here we show zebrafish spaca6 encodes sperm membrane protein which fertilization. Zebrafish knockout...