A5064370128- Sperm and Testicular Function
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microtubule and mitosis dynamics
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Plant Reproductive Biology
- Genetic and Kidney Cyst Diseases
- Pediatric Hepatobiliary Diseases and Treatments
- Neuroendocrine regulation and behavior
- Erythrocyte Function and Pathophysiology
- Genetic Mapping and Diversity in Plants and Animals
- Cancer Mechanisms and Therapy
- Marine Biology and Ecology Research
- Hemoglobinopathies and Related Disorders
- Hepatocellular Carcinoma Treatment and Prognosis
- GABA and Rice Research
- Gallbladder and Bile Duct Disorders
- Renal and related cancers
- Epigenetics and DNA Methylation
- Rice Cultivation and Yield Improvement
- Liver Disease Diagnosis and Treatment
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Lipids, and Metabolism
Osaka University
2019-2025
Soochow University
2023
First Affiliated Hospital of Soochow University
2023
Guangxi Medical University
2021-2022
Fourth Affiliated Hospital of Guangxi Medical University
2017-2022
Yunnan Academy of Agricultural Sciences
2020
Chongqing University
2019
University of Newcastle Australia
2017
Tangshan People's Hospital
2011
Significance The sperm−oocyte fusion step is important to transport the male genome into oocytes. So far, IZUMO1 and FIMP have been identified as fusion-related proteins in spermatozoa, but molecular mechanisms underpinning all of required for this essential process remain unclear. In study, using CRISPR-Cas9−mediated gene knockouts mice, we discover that sperm SOF1, TMEM95, SPACA6 are fertility. As these genes conserved among mammals including human, they may explain not only also...
Sperm-oocyte fusion is a critical event in mammalian fertilization, categorized by three indispensable proteins. Sperm membrane protein IZUMO1 and its counterpart oocyte JUNO make complex allowing sperm to interact with the oocyte, subsequent sperm-oocyte fusion. Oocyte tetraspanin CD9 also contributes However, process cannot be explained solely these essential factors. In this study, we focused on analyzing testis-specific gene 4930451I11Rik generated mutant mice using CRISPR/Cas9 system....
Abstract More than 1000 genes are predicted to be predominantly expressed in mouse testis, yet many of them remain unstudied terms their roles spermatogenesis and sperm function essentiality male reproduction. Since individually indispensable factors can provide important implications for the diagnosis genetically related idiopathic infertility may serve as candidate targets development nonhormonal contraceptives, our laboratories continuously analyze functions testis-enriched vivo by...
Abstract The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show both mouse and zebrafish DCST1 DCST2 are necessary sperm to fertilize egg, similar their orthologs SPE-42 SPE-49 C. elegans Sneaky D. melanogaster . Mouse Dcst1 Dcst2 single knockout (KO) able undergo acrosome reaction normal relocalization IZUMO1, an essential factor fusion, equatorial segment. While KO...
Following the fertilization of an egg by a single sperm, coat or zona pellucida (ZP) hardens and polyspermy is irreversibly blocked. These events are associated with cleavage N-terminal region (NTR) glycoprotein ZP2, major subunit ZP filaments. ZP2 processing thought to inactivate sperm binding ZP, but its molecular consequences connection hardening unknown. Biochemical structural studies show that triggers oligomerization. Moreover, structure native vertebrate filament, combined AlphaFold...
Abstract Background Adenosine deaminase domain containing 2 (ADAD2) is a testis‐specific protein composed of double‐stranded RNA binding and non‐catalytic adenosine domain. A recent study showed that ADAD2 indispensable for the male reproduction in mice. However, detailed functions remain elusive. Objectives This aimed to investigate cause sterility Adad2 mutant mice understand molecular ADAD2. Materials methods homozygous mouse lines, –/– Δ/Δ , were generated by CRISPR/Cas9. Western...
Sperm acrosomal membrane proteins, such as Izumo sperm–egg fusion 1 (IZUMO1) and sperm acrosome-associated 6 (SPACA6), play essential roles in mammalian gamete binding or fusion. How their biosynthesis is regulated during spermiogenesis has largely remained elusive. Here, we show that 1700029I15Rik knockout male mice are severely subfertile spermatozoa do not fuse with eggs. a type-II transmembrane protein expressed early round spermatids but mature spermatozoa. It interacts proteins...
Abstract Mammalian spermatozoa are produced in the testis through spermatogenesis and matured epididymis to acquire fertilizing ability. Spermatozoa ejaculated migrate from uterus oviducts fuse with oocytes. Although over 2000 genes expressed abundantly mouse testes, responsible for male fertility not yet fully clarified. Here, we focused on two testis-enriched serine protease genes, Serine (Prss) 51 Prss55, which overlap their gene loci partially both mice humans. To characterize functions...
Cilia and flagella are ancient structures that achieve controlled motor functions through the coordinated interaction based on microtubules some attached projections. Radial spokes (RSs) facilitate beating motion of these organelles by mediating signal transduction between dyneins a central pair (CP) singlet microtubules. RS complex isolation from Chlamydomonas axonemes enabled detection 23 radial spoke proteins (RSP1-RSP23), although roles remain unknown. Recently, RSP15 has been reported...
Tmem95 encodes a sperm acrosomal membrane protein, whose knockout has male-specific sterility phenotype in mice. murine can bind to, but do not fuse with, eggs. How TMEM95 plays role fusion of and eggs remained elusive. Here, we utilize penetration assay as model system to investigate the function human TMEM95. We show that binds hamster egg membranes, providing evidence for receptor on Using X-ray crystallography, reveal an evolutionarily conserved, positively charged region putative...
In sexually reproducing organisms, the genetic information is transmitted from one generation to next via merger of male and female gametes. Gamete fusion a two-step process involving membrane recognition apposition through ligand-receptor interactions lipid mixing mediated by proteins. HAP2 (also known as GCS1) bona fide gamete fusogen in flowering plants protists. vertebrates, multitude surface proteins have been demonstrated be pivotal for sperm-egg fusion, yet none them exhibit typical...
ABSTRACT Fertilization, the fusion of sperm and egg, is essential for sexual reproduction. While several proteins have been demonstrated to be binding gametes in vertebrates, molecular mechanisms driving this key process are poorly understood. Here, we performed a protein interaction screen using AlphaFold-Multimer uncover protein-protein interactions fertilization. This resulted prediction trimeric complex composed fertilization factors Izumo1 Spaca6, Tmem81, previously not implicated We...
Abstract Developing a safe and effective male contraceptive remains challenge in the field of medical science. Molecules that selectively target reproductive tract whose targets are indispensable for function serve among best candidates novel non-hormonal method. To determine these genes vivo, mutant mice carrying disrupted testis- or epididymis-enriched were generated by zygote microinjection electroporation CRISPR/Cas9 components. Male fecundity was determined consecutively pairing...
Fertilization occurs as the culmination of multi-step complex processes. First, mammalian spermatozoa undergo acrosome reaction to become fusion-competent. Then, acrosome-reacted penetrate zona pellucida and adhere finally fuse with egg plasma membrane. IZUMO1 is first sperm protein proven be essential for sperm-egg fusion in mammals, Izumo1 knockout mouse but fail oolemma. However, function other species remains largely unknown. Here, we generated rats by CRISPR/Cas9 found male were...
Background: Recent studies highlight the carcinogenesis role of SHC-adaptor protein 1 (SHC1) in cancer initiation, development, and progression. However, its aberrant expression, diagnostic prognostic value remain unknown a variety tumors. Methods: The SHC1 expression profiles were analyzed using GTEx database, TCGA Oncomine CPTAC database. survival analysis was conducted GEPIA2, Kaplan-Meier Plotter, UALCAN, PrognoScan. values calculated with "pROC" package R software. genetic alteration...
Sperm morphogenesis is a tightly regulated differentiation process, disruption of which leads to sperm malfunction and male infertility. Here, we show that Tex38 knockout (KO) mice are infertile. KO spermatids exhibit excess retention residual cytoplasm around the head, resulting in abnormal morphology with backward head bending. TEX38 interacts colocalizes ZDHHC19, testis-enriched acyltransferase catalyzing protein S-palmitoylation, at plasma membrane spermatids. ZDHHC19 each downregulated...
Sperm IZUMO1 binds to egg JUNO, and this interaction is essential for mammalian fertilization. Isolated from a female mouse immunized with syngeneic sperm, the antisperm antibody OBF13 recognizes inhibits murine How interferes sperm-egg interactions was unknown. Here, we present X-ray crystal structure of in complex OBF13. apex four-helix domain IZUMO1, distant JUNO-binding site. Our OBF13-bound resembles apo-IZUMO1 differs JUNO. We identify that carries low level somatic hypermutation,...
Abstract Spermatogenesis is a complex developmental process that involves the proliferation of diploid cells, meiotic division, and haploid differentiation. Many genes are shown to be essential for male fertility using knockout (KO) mice; however, there still remain analyzed elucidate their molecular mechanism roles in spermatogenesis. Calcium- integrin-binding protein 1 (CIB1) ubiquitously expressed possesses three paralogs: CIB2, CIB3, CIB4. It reported Cib1 KO mice sterile due impaired In...
Mammalian sperm flagellum contains the midpiece characterized by a mitochondrial sheath that packs tightly around axoneme and outer dense fibers. Mitochondria are known as "powerhouse" of cell, produce ATP through tricarboxylic acid (TCA) cycle oxidative phosphorylation (OXPHOS). However, contribution TCA OXPHOS to motility male fertility is less clear. Cytochrome c oxidase (COX) an oligomeric complex localized within inner membrane, terminal enzyme electron transport chain in eukaryotes....
Temperate japonica rice plays a very important role in food security. In this study, set of 191 temperate accessions from 30 countries were planted two sites China, and 12 agronomic traits measured. The results showed wide range variation for the Most are short; three with large panicles seven grains identified. Sixty-two quantitative trait loci (QTLs) identified that Among them, twenty-one QTLs both experiments, 41 only one site. Some known genes located QTL regions our study. SNP markers...