A5014020825- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Rice Cultivation and Yield Improvement
- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Agricultural Practices and Plant Genetics
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Genetics, Bioinformatics, and Biomedical Research
- GABA and Rice Research
- Genetics and Plant Breeding
- Genomics and Rare Diseases
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Light effects on plants
- Genetic diversity and population structure
SciGenom Labs (India)
2017-2023
University of Hyderabad
2009-2022
Nitric oxide (NO) plays a pivotal role in growth and disease resistance plants. It also acts as secondary messenger signaling pathways for several plant hormones. Despite its clear regulating development, fruit development is not known. In an earlier study, we described short root (shr) mutant of tomato, whose phenotype results from hyperaccumulation NO. The molecular mapping localized shr locus 2.5 Mb region chromosome 9. showed sluggish growth, with smaller leaves, flowers was less fertile...
The identification of mutations in targeted genes has been significantly simplified by the advent TILLING (Targeting Induced Local Lesions In Genomes), speeding up functional genomic analysis animals and plants. Next-generation sequencing (NGS) is gradually replacing classical for mutation detection, as it allows a large number amplicons short durations. NGS approach was used to identify population Solanum lycopersicum (tomato) that doubly mutagenized ethylmethane sulphonate (EMS)....
TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetics procedure for identifying point mutations selected gene(s) amplified from mutagenized population using high-throughput detection platforms such as slab gel electrophoresis, capillary electrophoresis or dHPLC. One essential pre-requisite genomic DNA isolation large PCR amplification of target genes. It also requires multiplexing isolated different individuals (pooling) typically 8-fold pools, mutation scanning, and to...
The data generated during a course of biological experiment/study can be sometimes massive and its management becomes quite critical for the success investigation undertaken. accumulation analysis such large datasets often tedious biologists lab technicians. Most current phenotype acquisition systems do not cater to specialized needs large-scale analysis. successful application genomic tools/strategies introduce desired traits in plants requires extensive precise phenotyping plant...
Abstract In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movement and chloroplast relocation. These photoresponses mediated two phot1 phot2. Phot1 mediates responses with sensitivity than phot2, phot2 specifically avoidance dark positioning responses. Here, we report isolation characterization of a Nonphototropic seedling1 (Nps1) mutant tomato (Solanum lycopersicum). The impaired low-fluence BL responses,...
Abstract In rice ( Oryza sativa L.), during the course of domestication, numerous beneficial alleles remain untapped in progenitor wild species and landraces. This study aims at uncovering these promising six key genes influencing yield, such as DEP1 , Ghd7 Gn1a GS 3, qSW5 sd1 by targeted resequencing 200 genotypes. all, 543 nucleotide variations including single polymorphisms insertion deletion were identified from genes. Of them, 225 novel alleles, which present only 91 that showed...
Samba Mahsuri (SM) is an Indian elite rice cultivar preferred by farmers and consumers primarily due to its superior 'eating cooking qualities' (ECQs), 'grain size shape' (GSS) traits. However, SM found be relatively stress susceptible the link between grain quality response remained unknown. We have investigated genetic basis of underlying mechanism these traits in through whole genome sequencing comparative analysis with IRGSP v1.0 as reference genome. SNPs/INDELs 946 known QTLs related...
World-wide grown and consumed tomato (Solanum lycopersicum) crop used as model system for new cultivar fruit development. Genetic genomic research of Indian cultivars will provide an insight to develop breeding strategies improvement. The present study aimed identify the high quality common unique single nucleotide polymorphisms (SNPs), in 9 different using double digestion restriction associated DNA sequencing (ddRAD-seq). Total 36,847,092 raw reads (3.68 GB) were generated all samples...
Mutagenesis based TILLING (MutTILL) is a reverse genetic approach that can be applied in plant research and breeding programs to broaden the variability for identification of novel mutant alleles specific genes interest with improved varieties.MutTILL method non-transgenic approach, further removing barriers marketing new varieties.It has been widely adapted different species across genera study gene function.Traditionally, work flow comprises mutagenesis, followed by scanning amplicon...
World-wide grown and consumed tomato (Solanum lycopersicum) is used as model crop for newcultivar fruit development. Genetic genomic studieson Indian cultivars will provide an insight that enable development of breeding strategies improvement. The present study aims to identifythe high quality common unique SNPs INDELs, in 9 different using double digest restriction site-associated DNA sequencing (ddRAD-seq). A total 36.8 million raw reads were generated selected average 94% high-quality...
Abstract In ripening tomato fruits, the leaf-specific carotenoids biosynthesis mediated by phytoene synthase 2 (PSY2) is replaced a fruit-specific pathway expression of two chromoplast-specific genes: 1 ( PSY1 ) and lycopene-β-cyclase CYCB ). Though both PSY2 genes express in functional role not known. To decipher whether PSY2-mediated carotenogenesis operates we blocked vivo activity lycopene-β-cyclases fruits several mutants CPTA (2-(4-Chlorophenylthio)triethylamine hydrochloride), an...