A5075018331- Plant Molecular Biology Research
- Antioxidant Activity and Oxidative Stress
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Postharvest Quality and Shelf Life Management
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Connexins and lens biology
- Light effects on plants
- Plant Micronutrient Interactions and Effects
- Calpain Protease Function and Regulation
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Genetics, Bioinformatics, and Biomedical Research
- Greenhouse Technology and Climate Control
- Plant Surface Properties and Treatments
- Phytochemicals and Antioxidant Activities
- Plant Pathogenic Bacteria Studies
University of Hyderabad
2015-2024
Genomics and Biotechnology of the Fruits Laboratory
2019-2020
Université de Toulouse
2019
Oregon State University
2011
University of Missouri
2001-2006
University of Missouri Health System
2006
Centre for Cellular and Molecular Biology
2003
The dietary carotenoids serve as precursor for vitamin A and prevent several chronic-degenerative diseases. carotenoid profiling is necessary to understand their importance on human health. However, the available high-performance liquid chromatography (HPLC) methods resolve major require longer analysis times do not adequately violaxanthin neoxanthin.A fast sensitive HPLC method was developed using a C30 column at 20°C with gradient consisting of methanol, methyl-tert-butyl ether water....
Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1 (high pigment1 [hp1]), Deetiolated1 (hp2), Zeaxanthin Epoxidase (hp3), and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled an increase chloroplast number size. However, little is known about the underlying mechanisms exaggerating carotenoid these mutants. A comparison proteome profiles from outer pericarp hp1 mutant...
Abstract Tomato fruit ripening is a complex metabolic process regulated by genetical hierarchy. A subset of this also modulated light signalling, as mutants encoding negative regulators phytochrome signal transduction show higher accumulation carotenoids. I n tomato, phytochromes are encoded multi‐gene family, namely PHYA , PHYB 1 2 PHYE and PHYF ; however, their contribution to development has not been examined. Using single phyA phyB1 phyB2 multiple phyAB1 phyB1B2 phyAB1B2 we compared the...
Nitric oxide (NO) plays a pivotal role in growth and disease resistance plants. It also acts as secondary messenger signaling pathways for several plant hormones. Despite its clear regulating development, fruit development is not known. In an earlier study, we described short root (shr) mutant of tomato, whose phenotype results from hyperaccumulation NO. The molecular mapping localized shr locus 2.5 Mb region chromosome 9. showed sluggish growth, with smaller leaves, flowers was less fertile...
The identification of mutations in targeted genes has been significantly simplified by the advent TILLING (Targeting Induced Local Lesions In Genomes), speeding up functional genomic analysis animals and plants. Next-generation sequencing (NGS) is gradually replacing classical for mutation detection, as it allows a large number amplicons short durations. NGS approach was used to identify population Solanum lycopersicum (tomato) that doubly mutagenized ethylmethane sulphonate (EMS)....
The role of ethylene in plant development is mostly inferred from its exogenous application. usage mutants affecting biosynthesis proffers a better alternative to decipher role. In tomato (Solanum lycopersicum), 1-aminocyclopropane carboxylic acid synthase2 (ACS2) key enzyme regulating ripening-specific biosynthesis. We characterised two contrasting acs2 mutants; acs2-1 overproduces ethylene, has higher ACS activity, and increased protein levels, while acs2-2 an underproducer, displays lower...
Domestication of tomato has resulted in large diversity fruit phenotypes. An intensive phenotyping 127 accessions from 20 countries revealed extensive morphological traits. The traits clustered the into nine classes and identified certain promising lines having desirable pertaining to total soluble salts (TSS), carotenoids, ripening index, weight shape. Factor analysis morphometric data Tomato Analyzer showed that shape is a complex trait shared by several factors. 100% variance between...
Abstract During seed germination, emerging roots display positive gravitropism and penetrate into the soil for nutrition anchorage. Tomato (Solanum lycopersicum) seeds germinated in presence of 1-methylcyclopropene (1-MCP), an inhibitor ethylene action, failed to insert Soilrite grew air, forming loops. Time-lapse video imaging showed that 1-MCP-grown root tips retained made contact with surface but Soilrite. Time-course studies revealed effect 1-MCP was most prominent when imbibition...
Phototropins, the UVA-blue light photoreceptors, endow plants to detect direction of and optimize photosynthesis by regulating positioning chloroplasts stomatal gas exchange. Little is known about their functions in other developmental responses. A tomato Non-phototropic seedling1 (Nps1) mutant, bearing an Arg495His substitution vicinity LOV2 domain phototropin1, dominant-negatively blocks phototropin1 The fruits Nps1 mutant were enriched carotenoids, particularly lycopene, compared with its...
The polycotyledon mutant of tomato (Lycopersicon esculentum L. cv Ailsa Craig) showed altered development during embryogenesis and vegetative reproductive phases. phenotype was pleiotropic included the formation extra cotyledons, changes in leaf shape, increased number flowers (indeterminacy) with abnormal floral organs, epiphyllous structures, gravitropism. earliest defects were observed at transition from globular to heart stage multiple cotyledons. Epidermal cells embryo smaller less...
TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetics procedure for identifying point mutations selected gene(s) amplified from mutagenized population using high-throughput detection platforms such as slab gel electrophoresis, capillary electrophoresis or dHPLC. One essential pre-requisite genomic DNA isolation large PCR amplification of target genes. It also requires multiplexing isolated different individuals (pooling) typically 8-fold pools, mutation scanning, and to...
An optimized protocol was developed for shotgun proteomics of tomato fruit, which is a recalcitrant tissue due to high percentage sugars and secondary metabolites. A number protein extraction fractionation techniques were examined optimal from fruits followed by peptide separation on nanoLCMS. Of all evaluated agents, buffer saturated phenol the most efficient. In-gel digestion (SDS-PAGE LCMS (GeLCMS)) phenol-extracted sample yielded maximal proteins. For in-solution digested samples, strong...
Abstract REPRESSOR OF UV-B PHOTOMORPHOGENESIS (RUP) negatively regulates UV- B signalling, yet its broader physiological roles in crop plants remain largely unexplored. We compared two tomato accessions bearing truncated RUP proteins— rup-1 and rup-2 ( rup- variants)—with the cultivar Arka Vikas (AV), which harbours native protein. Seedlings of rup -variants exhibited enhanced tolerance to supplemental light. Red ripe (RR) fruits showed significantly elevated carotenoid folate levels AV....
Members of the tomato clade exhibit a wide diversity in fruit color, but mechanisms governing inter-species coloration are largely unknown. The carotenoid profiles, carotenogenic gene expression and proteome profiles green-fruited Solanum habrochaites (SH), orange-fruited S. galapagense, red-fruited pimpinellifolium were compared with cultivated [S. lycopersicum cv. Ailsa Craig (SL)] to decipher molecular basis diversity. Green-fruited SH, though it showed normal chromoplast-specific...
The data generated during a course of biological experiment/study can be sometimes massive and its management becomes quite critical for the success investigation undertaken. accumulation analysis such large datasets often tedious biologists lab technicians. Most current phenotype acquisition systems do not cater to specialized needs large-scale analysis. successful application genomic tools/strategies introduce desired traits in plants requires extensive precise phenotyping plant...
Abstract In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movement and chloroplast relocation. These photoresponses mediated two phot1 phot2. Phot1 mediates responses with sensitivity than phot2, phot2 specifically avoidance dark positioning responses. Here, we report isolation characterization of a Nonphototropic seedling1 (Nps1) mutant tomato (Solanum lycopersicum). The impaired low-fluence BL responses,...
The ancestor of tomato underwent whole genome triplication ca. 71 Myr ago followed by widespread gene loss. However, few the triplicated genes are retained in modern day including lycopene beta cyclase that mediates conversion to β-carotene. fruit specific β-carotene formation is mediated a chromoplast-specific paralog (CYC-B) gene. Presently limited information available about how variations CYC-B contributed its neofunctionalization. clade contained several SNPs and In-Dels coding sequence...
Induced mutations accelerate crop improvement by providing novel disease resistance and yield alleles. However, the alleles with no perceptible phenotype but have an altered function remain hidden in mutagenized plants. The whole-genome sequencing (WGS) of individuals uncovers complete spectrum genome. Genome-wide induced mutation resources can improve targeted breeding tomatoes facilitate functional genomics. In this study, we sequenced 132 doubly ethyl methanesulfonate (EMS)-mutagenized...