A5025719570- 3D Printing in Biomedical Research
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Gene Regulatory Network Analysis
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Digital Marketing and Social Media
- Mesenchymal stem cell research
- Biotechnology and Related Fields
- Cardiac Ischemia and Reperfusion
- Neuroinflammation and Neurodegeneration Mechanisms
- Peripheral Neuropathies and Disorders
- 14-3-3 protein interactions
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Multiple Sclerosis Research Studies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Bioinformatics and Genomic Networks
- Axon Guidance and Neuronal Signaling
- Technology Adoption and User Behaviour
- Impact of Technology on Adolescents
- Coronary Interventions and Diagnostics
University of Virginia
2016-2025
Albemarle (United States)
2025
Johns Hopkins University
2012-2019
Johns Hopkins Medicine
2019
Hospital for Sick Children
2019
Significance What new properties may result from collective cell behavior, and how these emerging capabilities influence shaping function of tissues, in health disease? Here, we explored questions the context epithelial branching morphogenesis. We show experimentally that, whereas individual mammary cells are incapable sensing extremely weak gradients a growth factor, cellular collectives organotypic cultures exhibit reliable, gradient-driven, directional growth. This underscores critical...
Numerous regulators of cardiomyocyte (CM) proliferation have been identified, yet how they coordinate during cardiac development or regeneration is poorly understood. Here, we developed a computational model the CM regulatory network to obtain key and systems-level understanding. The defines five modules (DNA replication, mitosis, cytokinesis, growth factor, Hippo pathway) integrates them into 72 nodes 88 reactions that correctly predicts 73 78 (93.6%) independent experiments from...
Significance Intercellular communication between cells in tissues is frequently mediated by secreted factors, with rates of secretion varying over time. Stem can be recruited and contribute to the reparative process diverse tissues. How this versatile cytoprotective effect achieved not well understood. Here we describe a simple-to-use platform estimate dynamics cellular paracrine signaling response wide range physiologically relevant perturbations. When injured cardiomyocytes were presented...
Significance EAE typically presents with hind-limb paralysis and is associated severe T cell-mediated inflammation axon injury throughout the spinal cord parts of brain. Because loss so rapid in this model, it difficult to intervene for purpose evaluating neuroprotective therapies. Here we describe a mild form that does not even meet threshold scoring on traditional scales, yet age results devastating neurodegenerative changes cord, including grey matter atrophy, neuron loss, synapse...
Abstract Collective cell responses to exogenous cues depend on cell-cell interactions. In principle, these can result in enhanced sensitivity weak and noisy stimuli. However, this has not yet been shown experimentally, and, little is known about how multicellular signal processing modulates single extracellular signaling inputs, including those guiding complex changes the tissue form function. Here we explored if communication enhance ability of ensembles sense respond gradients chemotactic...
It is now increasingly recognized that a variety of stem cells offer therapeutic benefit to injured tissues via paracrine signaling. Paracrine signaling secreted ligands an important mediator cell function. has been challenge precisely measure cellular secretions in relevant physiological contexts. Here we introduce high throughput platform combining protein microprinting and microfluidics, μFLISA, directly secretion estimate the kinetics for detected secretome (Fig. 1A). μFLISA design...
Angiogenesis is a complex morphogenesis process coordinating endothelial cell (EC) signaling through matrix/integrin pathways and growth factor/receptor pathways, including the vascular factor (VEGF) family their receptors. VEGF presented to ECs in two modes – freely diffusing or bound extracellular matrix (ECM). While most studies have focused on diffusible VEGF, matrix‐bound also induces receptor activation resulting unique signaling. Using combination of experimental computational...
Abstract Cardiac diseases are characterized by the inability of adult mammalian hearts to overcome loss cardiomyocytes (CMs). Current knowledge in cardiac regeneration lacks a clear understanding molecular systems determining whether CMs will progress through cell cycle proliferate. Here, we developed computational model proliferation signaling that identifies key regulators and provides systems-level cardiomyocyte regulatory network. This defines five networks (DNA replication, mitosis,...
Rationale: Heart failure is caused by the inability of adult mammalian hearts to overcome loss cardiomyocytes (CMs). This due partly limited proliferative capacity CMs, which exit cell cycle and do not undergo division. Current knowledge in cardiac regeneration lacks an understanding molecular regulatory networks that determine whether CMs will progress through proliferate. Our goal use computational modeling understand expression activation levels core network, specifically cyclins...