A5101848511- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Mechanisms of cancer metastasis
- Genetics and Neurodevelopmental Disorders
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Ubiquitin and proteasome pathways
- RNA regulation and disease
University of Washington
2021-2024
Ministry of Agriculture and Rural Affairs
2020
China Agricultural University
2020
Measurements of gene expression or signal transduction activity are conventionally performed using methods that require either the destruction live imaging a biological sample within timeframe interest. Here we demonstrate an alternative paradigm in which such activities stably recorded to genome. Enhancer-driven genomic recording transcriptional multiplex (ENGRAM) is based on signal-dependent production prime editing guide RNAs mediate insertion signal-specific barcodes (symbols) into...
Recently-emerged base editing technologies could create single mutations at precise genomic positions without generation DNA double strand breaks. Herbicide resistant have been successfully introduced to different plant species, including Arabidopsis, watermelon, wheat, potato and tomato via C T (or G A on the complementary strand) editors (CBE) P197 position of endogenous acetolactate synthase (ALS) genes. Additionally, conversion another conserved amino acid S653 ALS gene confer tolerance...
Abstract Technologies that precisely delete genomic sequences in a programmed fashion can be used to study function as well potentially for gene therapy. The leading contemporary method deletion uses CRISPR/Cas9 and pairs of guide RNAs (gRNAs) generate two nearby double-strand breaks, which is often followed by the intervening sequence during DNA repair. However, this approach inefficient imprecise, with errors including small indels at target sites unintended large deletions more complex...