A5068641535- Vector-borne infectious diseases
- Insect and Pesticide Research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Analytical Chemistry and Sensors
- Pesticide Exposure and Toxicity
- Ion channel regulation and function
- Electrochemical sensors and biosensors
- Viral Infections and Vectors
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- Bacillus and Francisella bacterial research
- Biosensors and Analytical Detection
- Mosquito-borne diseases and control
- Neuroscience and Neuropharmacology Research
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Nicotinic Acetylcholine Receptors Study
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Vector-Borne Animal Diseases
- bioluminescence and chemiluminescence research
- Advanced Biosensing Techniques and Applications
Czech Academy of Sciences, Biology Centre
2014-2025
Institute of Parasitology
2014-2025
Czech Academy of Sciences, Institute of Microbiology
2024
Mitre (United States)
2018-2022
Veterinary Research Institute
2016-2021
University of South Bohemia in České Budějovice
2019-2021
Czech Academy of Sciences
2013-2020
The University of Texas at San Antonio
1989-2020
Texas Center for Infectious Disease
2020
Excet (United States)
2016
ABSTRACT Bacterial cell-to-cell communication facilitates coordinated expression of specific genes in a growth rate-II and cell density-dependent manner, process known as quorum sensing. While the discovery diffusible Escherichia coli signaling pheromone, termed autoinducer 2 (AI-2), has been made along with several sensing genes, overall number coordination controlled by through AI-2 signal not studied systematically. We investigated global changes mRNA abundance elicited molecule use luxS...
The regulatory network for the uptake of Escherichia coli autoinducer 2 (AI-2) is comprised a transporter complex, LsrABCD; its repressor, LsrR; and cognate signal kinase, LsrK. This an integral part AI-2 quorum-sensing (QS) system. Because LsrR LsrK directly regulate uptake, we hypothesized that they might play wider role in regulating other QS-related cellular functions. In this study, characterized physiological changes due to genomic deletion lsrR lsrK. We discovered many genes were...
ABSTRACT Bacterial autoinducer 2 (AI-2) is proposed to be an interspecies mediator of cell-cell communication that enables cells operate at the multicellular level. Many environmental stimuli have been shown affect extracellular AI-2 levels, carbon sources being among most important. In this report, we show both synthesis and uptake in Escherichia coli are subject catabolite repression through cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription lsr (for “ luxS regulated”)...
Ticks and tick-borne diseases are a growing burden worldwide vaccines effective control interventions. Vaccine formulations with tick antigens such as BM86/BM95 (BM) Subolesin (SUB) have shown reduction in fitness infestation immunized hosts. However, antigen combination is challenging approach to improve vaccine efficacy (E) against multiple species. Herein, silico music algorithms were integrated model BM-SUB protein-protein interactions apply quantum vaccinology for combining protective...
ABSTRACT The bacterial quorum-sensing autoinducer 2 (AI-2) has received intense interest because the gene for its synthase, luxS , is common among a large number of species. We have identified -controlled genes in Escherichia coli under two different growth conditions using DNA microarrays. Twenty-three were affected by deletion presence glucose, and 63 influenced absence glucose. Minimal overlap these sets suggests role condition dependent. Under latter condition, metE gene, lsrACDBFG...
Bacteriophage MS2 is used in place of pathogenic viruses a wide variety studies that range from testing compounds for disinfecting surfaces to studying environmental transport and fate groundwater. also as pathogen simulant the research, development, (including open air tests) methods, systems, devices detection pathogens both battlefield homeland defense settings. PCR often either an integral part such systems or reference method assess sensitivity specificity microbial detection. To...
Tick-borne encephalitis virus (TBEV) is a leading cause of human neuroinfections in Europe and Northeast Asia. There are no antiviral therapies for treating TBEV infection. A series nucleoside analogues was tested the ability to inhibit replication porcine kidney cells neuroblastoma cells. The interactions three with viral polymerase were simulated using advanced computational methods. 7-deaza-2'-C-methyladenosine (7-deaza-2'-CMA), 2'-C-methyladenosine (2'-CMA), 2'-C-methylcytidine (2'-CMC)...
As an ecological adaptation venoms have evolved independently in several species of Metazoa. haematophagous arthropods ticks are mainly considered as ectoparasites due to directly feeding on the skin animal hosts. Ticks major importance since they serve vectors for diseases affecting humans and livestock animals. rarely venomous animals despite that tick saliva contains protein families present taxa many Ixodida genera can induce paralysis other types toxicoses. Tick was previously proposed...
Epigenetic mechanisms have not been characterized in ticks despite their importance as vectors of human and animal diseases worldwide. The objective this study was to characterize the histones histone modifying enzymes (HMEs) tick vector Ixodes scapularis role during Anaplasma phagocytophilum infection. We first identified 5 34 HMEs I. comparison with similar proteins model organisms. Then, we used transcriptomic proteomic data analyze mRNA protein levels response A. infection tissues...
Abstract The carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) is produced in all mammals except for humans, apes and old world monkeys that lost the ability to synthetize this carbohydrate. Therefore, humans can produce high antibody titers against α-Gal. Anti-α-Gal IgE antibodies have been associated with tick-induced allergy (i.e. α-Gal syndrome) anti-α-Gal IgG/IgM may be involved protection malaria, leishmaniasis Chagas disease. on tick salivary proteins plays an important role etiology of...
We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using following motif: (His)(6)-GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively, (His)(6) a histidine affinity ligand purification, EK an enterokinase cleavage site recovering protein-of-interest from fusion. The CAT OPH products ( approximately 63 kDa GFP/CAT 70 GFP/OPH) were...
ABSTRACT Numerous gram-negative bacteria employ a cell-to-cell signaling mechanism, termed quorum sensing, for controlling gene expression in response to population density. Recently, this phenomenon has been discovered Escherichia coli , and while pathogenic E. utilize sensing regulate pathogenesis (i.e., of virulence genes), the role nonpathogenic is less clear, particular, there no information regarding during overexpression recombinant proteins. The production autoinducer AI-2, molecule...
This study proposes the use of carbon nanotubes (CNTs) as reinforcement to enhance mechanical properties a polylactide−caprolactone copolymer (PLC) matrix. Biological interaction PLC−CNT composites with human osteoblast cells is also investigated. Addition 2 wt % CNT shows very uniform dispersion in matrix, whereas 5 severe agglomeration and high porosity. PLC−2 composite an improvement increase elastic modulus by 100% tensile strength 160%, without any adverse effect on ductility up 240%...
Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses that inflict serious fatal infections in companion animals livestock. The aim this paper was to phylogeneticaly characterise a new isolated from Rhipicephalus (Boophilus) microplus Minas Gerais, Brazil.The agent hemolymph (B.) engorged females had been collected naturally infested cattle farm state Brazil. This then established cultured IDE8 tick cells. molecular phylogenetic analysis...
Escherichia coli were genetically modified to enable programmed motility, sensing, and actuation based on the density of features nearby surfaces. Then, calculated feature density, these cells expressed marker proteins indicate phenotypic response. Specifically, site-specific synthesis bacterial quorum sensing autoinducer-2 (AI-2) is used initiate recruit motile cells. In our model system, we rewired E. coli's AI-2 signaling pathway direct bacteria a squamous cancer cell line head neck...
Viral RNA dependent polymerases (vRdPs) are present in all viruses; unfortunately, their sequence similarity is too low for phylogenetic studies. Nevertheless, vRdP protein structures remarkably conserved. In this study, we used the structural of vRdPs to reconstruct evolutionary history. The major strength work unifying and data into a single quantitative analysis, using powerful Bayesian approach. resulting phylogram demonstrates that RNA-dependent DNA (RdDPs) viruses within Retroviridae...
The obligate intracellular pathogen,