A5065937259- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Virus-based gene therapy research
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Neurofibromatosis and Schwannoma Cases
- BRCA gene mutations in cancer
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- Cancer Genomics and Diagnostics
- Advanced biosensing and bioanalysis techniques
- Vascular Malformations and Hemangiomas
- RNA Interference and Gene Delivery
- Gene expression and cancer classification
- RNA modifications and cancer
- HER2/EGFR in Cancer Research
- Cancer Research and Treatments
- Neuroblastoma Research and Treatments
- Cardiac tumors and thrombi
- T-cell and Retrovirus Studies
- Animal Genetics and Reproduction
- Sarcoma Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
The Barbara Ann Karmanos Cancer Institute
2013-2024
Wayne State University
2015-2024
Michigan United
2011-2024
Weatherford College
2007-2009
Pediatrics and Genetics
2008
Institute of Molecular Genetics
2006
The University of Texas MD Anderson Cancer Center
1990-2003
Cancer Genetics (United States)
1999
Drexel University
1998
Nippon Dental University Hospital
1995
Familial cancer syndromes have helped to define the role of tumor suppressor genes in development cancer. The dominantly inherited Li-Fraumeni syndrome (LFS) is particular interest because diversity childhood and adult tumors that occur affected individuals. rarity high mortality LFS precluded formal linkage analysis. alternative approach was select most plausible candidate gene. gene, p53, studied previous indications this gene inactivated sporadic (nonfamilial) forms cancers are associated...
As normal cells progress toward malignancy, they must switch to an angiogenic phenotype attract the nourishing vasculature that depend on for their growth. In cultured fibroblasts from Li-Fraumeni patients, this was found coincide with loss of wild-type allele p53 tumor suppressor gene and be result reduced expression thrombospondin-1 (TSP-1), a potent inhibitor angiogenesis. Transfection assays revealed can stimulate endogenous TSP-1 positively regulate promoter sequences. These data...
Studies of yeast have shown that the SIR2 gene family is involved in chromatin structure, transcriptional silencing, DNA repair, and control cellular life span. Our functional studies human SIRT2, a homolog product gene, indicate it plays role mitosis. The SIRT2 protein NAD-dependent deacetylase (NDAC), abundance which increases dramatically during mitosis multiply phosphorylated at G(2)/M transition cell cycle. Cells stably overexpressing wild-type but not missense mutants lacking NDAC...
ABSTRACT As the major proteins of adult keratinocytes, keratins provide biochemical markers for exploring mouse epidermal embryogenesis. Here, we used a modified method whole-mount in situ hybridization to track skin-specific expression endogenous keratin mRNAs through-out To monitor transcriptional regulation, coupled this with β-galactosidase human promoter-driven transgene. These studies have radically changed our perception how program gene becomes established during development....
Abstract Our previous studies have shown that human skin cancers occurring on sun‐exposed body sites frequently contain activated Ha‐ ras oncogenes capable of inducing morphologic and tumorigenic transformation NIH 3T3 cells. In this study, we analyzed primary squamous cell carcinomas (SCCs) basal (BCCs) for mutations in codons 12, 13, 61 , Ki‐ N‐ by amplification genomic tumor DNAs the polymerase chain reaction, followed dot‐blot hybridization to synthetic oligonucleotide probes designed...
Differentiation of skeletal muscle involves withdrawal myoblasts from the cell cycle, fusion to form myotubes, and coordinate expression a variety muscle-specific gene products. Fibroblast growth factor type beta transforming specifically inhibit myogenesis; however, transmembrane signaling pathways responsible for suppression differentiation by these factors remain elusive. Because ras proteins have been implicated in transduction signals across plasma membrane, we used DNA-mediated...
Abstract A noninvasive screening test would significantly facilitate early detection of epithelial ovarian cancer. This study used a combination high-throughput selection and array-based serologic many antigens indicative the presence cancer, thereby using immune system as biosensor. involved biopanning an cancer phage display library serum immunoglobulins from patient bait. Protein macroarrays containing 480 these selected antigen clones revealed 65 that interacted with in sera 32 patients...
Abstract Cancer research has previously focused on the identification of specific genes and pathways responsible for cancer initiation progression based prevailing viewpoint that is caused by a stepwise accumulation genetic aberrations. This viewpoint, however, not consistent with clinical finding tumors display high levels heterogeneity distinctive karyotypes. We show chromosomal instability primarily generates stochastic karyotypic changes leading to random cancer. was accomplished tracing...
Cellular senescence is a cell cycle arrest accompanied by high expression of cyclin dependent kinase inhibitors which counteract overactive growth signals, serves as tumor suppressive mechanism. Senescence can be result telomere shortening (natural or replicative senescence) DNA damage resulting from exogenous stressors (induced senescence). Here, we performed gene profiling through RNA-seq senescence, adriamycin-induced H2O2-induced and 5-aza-2-deoxycytidine-induced in order to profile the...
MCAM/MUC18 is a cell-surface glycoprotein of 113 kDa, originally identified as melanoma antigen, whose expression associated with tumor progression and the development metastatic potential. We have previously shown that enforced in primary cutaneous led to increased growth potential nude mice. The mechanism for up-regulation during unknown. Here we show highly cells correlates loss transcription factor AP-2. promoter contains four binding sites AP-2, electrophoretic mobility shift assay gels...
Early passages of the human teratocarcinoma cell line PA1 are not tumorigenic in nude mice, while late are. A transforming gene present cells was isolated as a biologically active molecular clone and is new isolate rasN locus. Its activity due to single G---A (G, guanine; A, adenine) point mutation at codon for amino acid 12 which changes glycine so that an aspartic residue expressed. In contrast passage (passages 106, 330, 338), DNA from early (passage 36) does yield foci transfection...
AP-2 is a retinoic acid-inducible and developmentally regulated activator of transcription. We have cloned an alternative transcript (AP-2B) from the human teratocarcinoma cell line PA-1, which encodes protein differing in C terminus previously isolated (AP-2A). This contains activation domain part DNA binding but lacks dimerization necessary for binding. Analysis overlapping genomic clones spanning entire gene proves that AP-2A AP-2B transcripts are alternatively spliced same gene. Both...
Overexpression of transcription factor AP-2 has been implicated in the tumorigenicity human teratocarcinoma cell lines PA-1 that contain an activated ras oncogene. Here we show evidence overexpression sequesters transcriptional coactivators which results self-inhibition. We identified AP-2-interacting proteins and determined whether these were for AP-2-mediated transcription. One such interacting protein is polyADP-ribose polymerase (PARP). PARP suppresses self-inhibition enhances activity...