A5100723085- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Biochemical and Molecular Research
- Aquaculture disease management and microbiota
- Cancer, Hypoxia, and Metabolism
- Advanced Electron Microscopy Techniques and Applications
- Adhesion, Friction, and Surface Interactions
- Coastal and Marine Management
- Environmental and Agricultural Sciences
- Polyomavirus and related diseases
- Viral Infections and Immunology Research
- Dynamics and Control of Mechanical Systems
- HIV Research and Treatment
- Coral and Marine Ecosystems Studies
- Invertebrate Immune Response Mechanisms
- Viral-associated cancers and disorders
- Protein purification and stability
- Evaluation Methods in Various Fields
Fuyang Normal University
2022
Hainan University
2022
Hunan Agricultural University
2019
University of Kansas
2006-2018
University of Alabama at Birmingham
2006
Scripps Research Institute
2001-2006
University of Utah
2006
Montana State University
2004-2005
Portland State University
2004
Institute of Biophysics
1999-2001
Grow your nanochemical dendrimers! Cowpea mosaic virus (see relief image derived from the X-ray structure) can be isolated its host plant in gram quantities and participates selective chemical reactions at specific sites of icosahedrally-arrayed coat protein. Mutant structures easily engineered, propagated, to provide particles with tailored reactivities. Applications for these areas catalysis materials science conceived.
Bacteriophages, herpesviruses, and other large double-stranded DNA (dsDNA) viruses contain molecular machines that pump into preassembled procapsids, generating internal capsid pressures exceeding, by 10-fold, of bottled champagne. A 17 angstrom resolution asymmetric reconstruction the infectious P22 virion reveals tightly spooled about portal dodecamer forces a conformation is significantly different from observed in isolated portals assembled ectopically expressed protein. We propose tight...
Of the three domains of life (Eukarya, Bacteria, and Archaea), least understood is Archaea its associated viruses. Many are extremophiles, with species that capable growth at some highest temperatures extremes pH all known organisms. Phylogenetic rRNA-encoding DNA analysis places many hyperthermophilic (species an optimum ≳80°C) base universal tree life, suggesting thermophiles were among first forms on earth. Very few viruses have been identified from as compared to Bacteria Eukarya. We...
Archaea and their viruses are poorly understood when compared with the Eukarya Bacteria domains of life. We report here crystal structure major capsid protein (MCP) Sulfolobus turreted icosahedral virus, an archaeal virus isolated from acidic hot spring (pH 2–4, 72–92°C) in Yellowstone National Park. The is nearly identical to MCP structures eukaryotic Paramecium bursaria Chlorella bacteriophage PRD1, shows a common fold mammalian adenovirus. Structural analysis architecture, determined by...
ABSTRACT Herpes simplex virus 1 (HSV-1), the prototypic member of herpesviruses, employs a virally encoded molecular machine called terminase to package viral double-stranded DNA (dsDNA) genome into preformed protein shell. The contains large subunit that is thought cleave concatemeric during packaging initiation and completion each cycle supply energy process via ATP hydrolysis. We have determined X-ray structure C-terminal domain large-subunit pUL15 (pUL15C) from HSV-1. shows fold...
Many DNA viruses use powerful molecular motors to cleave concatemeric viral into genome-length units and package them preformed procapsid powered by ATP hydrolysis. Here we report the structures of DNA-packaging motor gp2 bacteriophage Sf6, which reveal a unique clade RecA-like ATPase domain an RNase H-like nuclease tethered regulatory linker domain, exhibiting strikingly distinct arrangement. The complexed with nucleotides reveal, at atomic detail, catalytic center embraced domain. activity...
We have developed methods for producing viral-based protein cages in high yield that are amenable to genetic modification. Expression of the structural Cowpea chlorotic mottle bromovirus (CCMV) using yeast-based Pichia pastoris heterologous expression system resulted assembly particles were visibly indistinguishable from virus produced natural host. shown a collection non-infectious CCMV coat mutants expressed P. assemble into viral with altered architectures and function. This provides an...
In herpesviruses and many bacterial viruses, genome-packaging is a precisely mediated process fulfilled by virally encoded molecular machine called terminase that consists of two protein components: A DNA-recognition component defines the specificity for packaged DNA, catalytic provides energy packaging reaction hydrolyzing ATP. The docks onto portal complex embedded in single vertex preformed viral shell procapsid, pumps DNA into procapsid through conduit formed portal. Here we report 1.65...
Many dsDNA viruses encode DNA-packaging terminases, each containing a nuclease domain that resolves concatemeric DNA into genome-length units. Terminase nucleases resemble the RNase H-superfamily nucleotidyltransferases in folds, and share two-metal-ion catalytic mechanism. Here we show residue K428 of bacteriophage terminase gp2 mediates binding metal cofactor Mg(2+). A K428A mutation allows visualization, at high resolution, ion mode with coupled-octahedral configuration active site,...
Significance The enormous prevalence of tailed double-stranded DNA bacteriophages on this planet is made possible by highly efficient self-assembly usually hundreds protein subunits into stable capsids. These capsids must stand with an internal pressure as a result the phage DNA-packaging process. Here we report complete atomic model headful bacteriophage Sf6 at 2.9 Å resolution determined electron cryo-microscopy. structure reveals DNA-inflated, tensed state robust yet flexible shell...
The multi-layered cell envelope structure of Gram-negative bacteria represents significant physical and chemical barriers for short-tailed phages to inject phage DNA into the host cytoplasm. Here we show that a DNA-injection protein bacteriophage Sf6, gp12, forms 465-kDa, decameric assembly in vitro. electron microscopic gp12 shows ~150-Å, mushroom-like architecture consisting crown domain tube-like domain, which embraces 25-Å-wide channel could precisely accommodate dsDNA. constricted...
The crystal structure of human muscle creatine kinase has been determined by the molecular-replacement method and refined at 3.5 Å resolution. structures both monomer dimer closely resemble those other known in family. Two types dimers, one with a non-crystallographic twofold symmetry axis crystallographic axis, were found to occur simultaneously crystal. These dimers form an infinite `double-helix'-like along unusual long 31 axis.
The single segment, double-stranded RNA genome of the L-A virus (L-A) yeast encodes two proteins: major coat protein Gag (76 kDa) and Gag-Pol fusion (180 kDa). icosahedral capsid is formed by 120 copies has architecture similar to that seen in reovirus, blue tongue rice dwarf inner shells. chemically removes m7GMP caps from host cellular mRNAs. Previously we identified a trench on outer surface included His154, which are covalently attached. Here report refined coordinates at 3.4 angstroms...
As a zone of interaction between land and sea, coastal zones are important carriers the marine economy, they focal point for strategies related to strengthening country by means sea. where sea most closely linked, become strategic area achieving sustainable expansion blue economic space. Coastal have some capacity cope with recover from adverse effects ecological pressure, which is ecosystem vulnerability. However, given vulnerability ecosystems existence considerable it necessary...
Kaposi's sarcoma-associated herpesvirus (KSHV), the etiological agent of sarcoma, belongs to Herpesviridae family, whose members employ a multicomponent terminase resolve nonparametric viral DNA into genome-length units prior their packaging. Homology modeling ORF29 C-terminal nuclease domain (pORF29C) and bacteriophage Sf6 gp2 have suggested an active site clustered with four acidic residues, D 476 , E 550 661 662 that collectively sequester catalytic divalent metal (Mn 2+ ) also provided...
Significance Many dsDNA bacteriophages possess a tail that encodes functions essential for viral life cycles. Assembly of such molecular machine has been shown to occur in sequential manner. Here, we report the high-resolution crystal structure adaptor protein gp7 from Shigella phage Sf6. Comparative structural studies reveal N-terminal portion undergoes rearrangement by repositioning two consecutive repeats conserved octad sequence motif, turning molecule preassembly state postassembly...
Climbing plants are an abundant and taxonomically diverse plant group that competes intensely with trees thus substantially affects forest diversity structure. The growth physiology of climbing largely depend on their root tip structure function. However, little is known regarding the mechanisms through which anatomical traits regulate diameter in plants. Therefore, our study sought to explore relationships between seven (e.g., cortex thickness stele diameter) three lianas vine species...