A5019004962- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neural dynamics and brain function
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Sepsis Diagnosis and Treatment
- Nicotinic Acetylcholine Receptors Study
- Long-Term Effects of COVID-19
- Intensive Care Unit Cognitive Disorders
- Diabetes Management and Research
- Neuroscience and Neural Engineering
- Respiratory Support and Mechanisms
- Hemodynamic Monitoring and Therapy
- Metal-Catalyzed Oxygenation Mechanisms
- Lipid Membrane Structure and Behavior
- Cardiac Arrest and Resuscitation
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Parathyroid Disorders and Treatments
- Cellular transport and secretion
- Retinal Development and Disorders
- Heart Rate Variability and Autonomic Control
- Traumatic Brain Injury and Neurovascular Disturbances
- Microbial metabolism and enzyme function
Taronga Conservation Society Australia
2025
Oregon Health & Science University
2010-2023
VA Portland Health Care System
2016-2023
ICON Technology & Process Consulting (United Kingdom)
2023
University of Oxford
2023
University of Portland
2007-2021
Pulmonary and Critical Care Associates
2007-2017
Portland VA Medical Center
2014
Northwestern University
2006-2011
Institute of Molecular and Cell Biology
2010
The Ca 2+ channel α 1A -subunit is a voltage-gated, pore-forming membrane protein positioned at the intersection of two important lines research: one exploring diversity channels and their physiological roles, other pursuing mechanisms ataxia, dystonia, epilepsy, migraine. -Subunits are thought to support both P- Q-type currents, but most direct test, null mutant, has not been described, nor it known which changes in neurotransmission might arise from elimination predominant delivery system...
Objectives: To determine whether extravascular lung water predicts survival in patients with early acute respiratory distress syndrome, to the relationship between and other markers of injury, examine if indexing predicted body weight (EVLWp) strengthens its discriminative power. Design: Extravascular injury were measured prospectively 19 sepsis-induced syndrome for 3 days. Setting: The intensive care units an academic tertiary referral hospital. Measurements Main Results: Lung score,...
Particulate methane monooxygenase (pMMO) is an integral membrane metalloenzyme that oxidizes to methanol in methanotrophic bacteria. Previous biochemical and structural studies of pMMO have focused on preparations from Methylococcus capsulatus (Bath) Methylosinus trichosporium OB3b. A a third organism, Methylocystis species strain M, has been isolated characterized. Both membrane-bound solubilized sp. M contain ~2 copper ions per 100 kDa protomer exhibit copper-dependent propylene...
The hypothalamus coordinates autonomic responses in part through arginine vasopressin (AVP) released medial nucleus tractus solitarius (NTS). However, the mechanisms and sites of AVP action within NTS pathways are uncertain. In brainstem slices, we activated solitary tract (ST) primary afferents to release glutamate tested whether modulated synaptic transmission second-order neurons. neurons were classified as second order by ST characteristics or presence anterograde tracers from peripheral...
Spontaneous release of glutamate is important for maintaining synaptic strength and controlling spike timing in the brain. Mechanisms regulating spontaneous exocytosis remain poorly understood. Extracellular calcium concentration ([Ca 2+ ] o ) regulates Ca entry through voltage-activated channels (VACCs) consequently a pivotal determinant action potential-evoked vesicle fusion. also enhances release, but via unknown mechanisms. Here we report that external triggers more weakly than evoked...
Presynaptic action potential spikes control neurotransmitter release and thus interneuronal communication. However, the properties dynamics of presynaptic in neocortex remain enigmatic because boutons are small direct patch-clamp recordings have not been performed. Here, we report from neocortical pyramidal neurons interneurons. Our data reveal rapid large potentials layer 5 fast-spiking interneurons reliably propagating into axon collaterals. For in-depth analyses, establish mature cultured...
The integral membrane enzyme particulate methane monooxygenase (pMMO) converts methane, the most inert hydrocarbon, to methanol under ambient conditions. 2.8-Å resolution pMMO crystal structure revealed three metal sites: a mononuclear copper center, dinuclear and nonphysiological zinc center. Although not found in structure, solution samples of also contain iron. We have used X-ray absorption spectroscopy analyze oxidation states coordination environments centers as-isolated (pMMOiso),...
Assessment of dynamic cerebral autoregulation (CA) requires continuous recording arterial blood pressure (ABP). In humans, noninvasive ABP recordings with the Finapres device have often been used for this purpose. We compared estimates CA derived from those invasive in aorta. Measurements finger (Finapres), intra-aortic (Millar catheter), surface ECG, transcutaneous CO2, and bilateral flow velocity (CBFV) middle arteries were simultaneously continuously recorded 27 patients scheduled...
In comparison to the well characterized role of principal subunit voltage-gated Ca 2+ channels, pore-forming, antagonist-binding α 1 subunit, considerably less is understood about how β subunits contribute neuronal channel function. We studied 3 major in neurons, by using a gene-targeting strategy. The deficient (β −/−) animals were indistinguishable from wild type (wt) with no gross morphological or histological differences. However, sympathetic −/− L- and N-type current was significantly...
A fresh extract of garlic (Allium sativum) was administered orally to human volunteers. At intervals, serum and urine were collected assayed for antifungal activity. The maximum tolerable dose determined be 25 ml extract. Larger amounts caused severe burning sensations in the esophagus stomach vomiting. After oral ingestion extract, anticandidal anticryptococcal activities detected undiluted 0.5 1 h after ingestion. No detectable activity found excreted at any time Oral is limited value...
Some aspects of overwintering and oogenesis in the pitcher-plant mosquito, Wyeomyia smithii (Coq.), were examined. The species was shown to overwinter a diapause state as third-instar larva. diapausing larvae within frozen ice cores host plant but they nevertheless be intolerant prolonged periods freezing at temperatures only few degrees below 0 °C. winter survival probably depends almost entirely on insulating benefits snow cover. Larval stable under short-day conditions very rapidly broken...
We aim to describe the outcomes after chronic subdural hematoma drainage (CSDH) management in a large cohort of patients on antithrombotic drugs, either antiplatelets or anticoagulants, at presentation and inform clinical decision making timing surgery recommencement these drugs. used data from previous UK-based multi-center, prospective study. Outcomes included recurrence within 60 days, functional outcome discharge, thromboembolic event during hospital stay. performed Cox regression...
At excitatory synapses, decreases in cleft [Ca] arising from activity-dependent transmembrane Ca flux reduce the probability of subsequent transmitter release. Intense neural activity, induced by physiological and pathological stimuli, disturb external microenvironment reducing extracellular ([Ca] o ) thus may impair neurotransmission. Increases activate calcium sensing receptor (CaSR) which turn inhibits nonselective cation channels at majority cortical nerve terminals. This pathway...
Nerve terminal invasion by an axonal spike activates voltage-gated channels, triggering calcium entry, vesicle fusion, and release of neurotransmitter. Ion channels activated at the shape presynaptic so regulate magnitude duration entry. Consequently characterization functional properties ion nerve terminals is crucial to understand regulation transmitter release. Direct recordings from small neocortical have revealed that external [Ca(2+)] ([Ca(2+)](o)) indirectly regulates a non-selective...
Acute renal failure is a common complication in critically ill patients and carries an increased morbidity mortality. N-acetylcysteine antioxidant anti-inflammatory agent that may counteract some of the pathophysiologic derangements shock states.To test whether administration N-acetylcysteine, compared with placebo, reduces incidence acute hypotensive patients.Prospective, randomized, double-blinded, placebo-controlled study.Intensive care units university tertiary hospital.One hundred...