A5015077278- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
- Plant Micronutrient Interactions and Effects
- Plant Physiology and Cultivation Studies
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Legume Nitrogen Fixing Symbiosis
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
Knoxville College
2023-2024
University of Tennessee at Knoxville
2023-2024
University of North Carolina at Chapel Hill
2012-2021
North Carolina State University
2021
University of North Carolina Health Care
2019
For self-pollinating plants to reproduce, male and female organ development must be coordinated as flowers mature. The Arabidopsis transcription factors AUXIN RESPONSE FACTOR 6 (ARF6) ARF8 regulate this complex process by promoting petal expansion, stamen filament elongation, anther dehiscence, gynoecium maturation, thereby ensuring that pollen released from the anthers is deposited on stigma of a receptive gynoecium. ARF6 induce jasmonate production, which in turn triggers expression MYB21...
The hormone auxin regulates growth largely by affecting gene expression. By studying Arabidopsis (Arabidopsis thaliana) mutants deficient in AUXIN RESPONSE FACTORS (ARFs), we have identified three ARF proteins that are required for auxin-responsive hypocotyl elongation. Plants these factors reduced responses to environmental conditions increase levels, including far-red-enriched light and high temperature. Despite having decreased responses, the ARF-deficient plants responded brassinosteroid...
The phytohormone cytokinin regulates diverse aspects of plant growth and development. Our understanding the metabolism perception has made great strides in recent years, mostly from studies model dicot
Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique easily and rapidly identify such indels. Sequence-identified mutations alter restriction enzyme recognition site readily distinguished from wild-type alleles cleaved amplified polymorphic sequence (CAPS) technique. If is created or altered by the mutation only one allele contains site, polymerase chain reaction...
SUMMARY The phytohormone cytokinin plays a significant role in nearly all aspects of plant growth and development. Cytokinin signaling has primarily been studied the dicot model Arabidopsis, with relatively little work done monocots, which include rice ( Oryza sativa ) other cereals agronomic importance. pathway is phosphorelay comprised histidine kinase receptors, authentic phosphotransfer proteins (AHPs) type‐B response regulators (RRs). Two negative have identified: type‐A RRs, are...
Genetic screens are powerful tools to dissect complex biological processes, but a rate-limiting step is often the cloning of targeted genes. Here, we present strategy, "mutagenomics," identify causal mutations from screen in high throughput fashion absence backcrossing. Mutagenomics initiated by sequencing genomes mutants identified, which then subjected three-stage pipeline. The first stage identifies sequence changes genes previously linked pathway. second uses heuristics derived...
Stomata are the pores on a leaf surface that regulate gas exchange. Each stoma consists of two guard cells whose movements pore opening and thereby control CO2 fixation water loss. Guard cell depend in part remodeling vacuoles, which have been observed to change from highly fragmented state fused morphology during stomata opening. This requires membrane fusion mechanism responds rapidly environmental signals, allowing plants respond diurnal stress cues. With vacuoles being both large...
Abstract Guard cell movements depend, in part, on the remodelling of vacuoles from a highly fragmented state to fused morphology during stomata opening. Indeed, full opening plant requires vacuole fusion occur. Fusion membranes is conserved process eukaryotes, with key roles played by two multi-subunit complexes: HOPS (homotypic and vacuolar protein sorting) SNARE (soluble NSF attachment receptor). tethering factor that thought chaperone SNAREs apposing into fusion-competent complex capable...
Abstract Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique easily and rapidly identify such indels. Sequence-identified mutations alter restriction enzyme recognition site distinguished from wild-type alleles cleaved amplified polymorphic sequence (CAPS) technique. If is created or altered by the mutation only one allele contains site, polymerase chain reaction...
Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an DNA fragment. A mutation that disrupts creates a restriction site will prevent cleavage by enzyme, allowing discrimination of wild-type and mutant alleles. In cases where no convenient is present, derived Amplified Polymorphic Sequence (dCAPS) assay can be used, mismatches the primer used to create...