A5037929039- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Cellular transport and secretion
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Light effects on plants
- Plant Stress Responses and Tolerance
- Calcium signaling and nucleotide metabolism
- Plant Virus Research Studies
- Ubiquitin and proteasome pathways
- Polar Research and Ecology
- Protein Degradation and Inhibitors
- Lipid metabolism and biosynthesis
- Spaceflight effects on biology
- CRISPR and Genetic Engineering
- Research in Cotton Cultivation
- Nuclear Structure and Function
- Plant pathogens and resistance mechanisms
- Analytical Chemistry and Chromatography
- Protist diversity and phylogeny
- Animal Genetics and Reproduction
- Innovative Microfluidic and Catalytic Techniques Innovation
- Polysaccharides and Plant Cell Walls
- Bacterial Genetics and Biotechnology
North Carolina State University
2015-2025
University of California, Riverside
2005-2014
Plant (United States)
2005-2014
International Center for Tropical Agriculture
2006
Carnegie Institution for Science
2005
University of Minnesota, Duluth
2005
University of California System
2003
Abstract Plasma agriculture details the role of nonthermal plasma in development plants from seeds to crops. Several publications reported enhanced plant growth, improved stress tolerance, and antimicrobial effects treatment plasma‐treated water. In this review, we present an overview recent literature put it context needs on ecosystem. We will discuss key developmental stages their needs, different growth environments hydroponics soilless soil substrates, microbiome. This review provides...
Low soil P availability is a primary constraint to common bean ( Phaseolus vulgaris L.) production in Latin America and Africa. Substantial genotypic variation adaptation low phosphorus (LP) has been linked with root traits that enhance the efficiency of foraging. The objectives this study were identify quantitative trait loci (QTLs) for accumulation associated architectural traits, facilitate genetic improvement reveal physiological relationships. Eighty‐six F 5.7 recombinant inbred lines...
Significance Leaves are the primary source of photoassimilate in crop plants. A precise understanding genetic architecture underlying leaf morphology is critical to engineering climate-resilient varieties. An ideal cotton cultivar would produce a lower canopy broad, normal leaves before transitioning an upper highly lobed, okra leaves. Here we show that major shapes controlled by locus, which encodes HD-Zip transcription factor Gossypium hirsutum LATE MERISTEM IDENTITY1-D1b ( GhLMI1-D1b )....
Inducible protein knockdowns are excellent tools to test the function of essential proteins in short time scales and capture role dynamic events. Current approaches destroy or sequester by exploiting plant biological mechanisms such as activity photoreceptors for optogenetics auxin-mediated ubiquitination auxin degrons. It follows that these not applicable plants light strong signals cells. We describe here an inducible degradation system named E3-DART E3-targeted Degradation Plant Proteins....
Chemical genomics is a powerful approach to dissect processes that may be intractable using conventional genetics because of gene lethality or redundancy. Recently, link has been established between endomembrane trafficking and gravitropism. To understand this link, we screened library 10,000 diverse chemicals for compounds affected the gravitropism Arabidopsis seedlings positively negatively. Sixty-nine 219 from primary screen were retested, 34 these confirmed inhibit enhance Four found...
Plants are unique in their ability to store proteins specialized protein storage vacuoles (PSVs) within seeds and vegetative tissues. Although plants use PSV during germination, before photosynthesis is fully functional, the roles of PSVs adult tissues not understood. Trafficking pathways lytic appear be distinct. Lytic analogous evolutionarily yeast mammalian lysosomes. However, it unclear whether trafficking has any analogy or mammals, nor ultrastructure known Arabidopsis tissue....
The vacuole is an essential organelle in plant cells, and its dynamic nature important for growth development. Homotypic membrane fusion required biogenesis, pollen germination, stomata opening, gravity perception. Known components of the machinery eukaryotes include SNARE proteins, Rab GTPases, phosphoinositides, homotypic vacuolar protein sorting (HOPS) tethering complex. HOPS function not well characterized plants, but roles embryogenesis tube elongation have been reported. Here, we show...
Tonoplast intrinsic proteins (TIPs) facilitate the membrane transport of water and other small molecules across plant vacuolar membrane, members this family are expressed in specific developmental stages tissue types. Delivery TIP to tonoplast is thought occur by vesicle–mediated traffic from endoplasmic reticulum vacuole, at least two pathways have been proposed, one that Golgi-dependent another Golgi-independent. However, mechanisms for trafficking remain poorly understood. Here we...
Abstract Leaf shape is spectacularly diverse. As the primary source of photo-assimilate in major crops, understanding evolutionary and environmentally induced changes leaf morphology are critical to improving agricultural productivity. The role cotton domestication unique, as breeders have purposefully selected for entire lobed morphs resulting from a single locus, okra ( L-D 1 ). locus not only importance Gossypium hirsutum L.), but through pioneering chimeric morphometric studies it has...
Vacuoles are dynamic compartments with constant fluctuations and transient structures such as trans-vacuolar strands bulbs. Bulbs highly spherical inside vacuoles that formed by multiple layers of membranes continuous the main tonoplast. We recently carried out a screen for mutants abnormal trafficking to vacuole or aberrant morphology. characterized regulator bulb biogenesis1-1 (rbb1-1), mutant in Arabidopsis contains increased numbers bulbs when compared parental control. rbb1-1 also...
Gravitropism in Arabidopsis shoots depends on the sedimentation of amyloplasts endodermis, and a complex interplay between vacuole F-actin. Gravity response is inhibited zigzag-1 (zig-1), mutant allele VTI11, which encodes SNARE protein involved fusion. zig-1 seedlings have fragmented vacuoles that fuse after treatment with wortmannin, an inhibitor phosphatidylinositol 3-kinase, underscore role phosphoinositides Using live-cell imaging vertical stage microscope, we determined young...
Plant vacuoles are essential and dynamic organelles, mechanisms of vacuole biogenesis fusion not well characterized. We recently demonstrated that Wortmannin, an inhibitor Phosphatidylinositol 3-Kinase (PI3K), induces the plant both in roots itt3/vti11 mutant alleles guard cells wild type Arabidopsis Fava bean. Here we used Fluorescence Recovery After Photobleaching (FRAP) to demonstrate independent organelles. Furthermore, fluorescent protein reporters bind specifically 3-Phosphate...
Abstract The spaceflight environment of the International Space Station poses a multitude stresses on plant growth including reduced gravity. Plants exposed to microgravity and other conditions ISS display root skewing, changes in gene expression protein abundance that may result cell wall composition, antioxidant accumulation modification anisotropy. Systematic studies address effects cellular organelles are lacking but altered numbers sizes vacuoles have been detected previous flights....
AbstractGene traps and enhancer provide a valuable tool for gene discovery. With this system, genes can be identified based solely on the expression pattern of an inserted reporter gene. The use gene, such as β-glucuoronidase (GUS), provides very sensitive assay identification tissue- cell-type specific patterns. In chapter, protocols examining documenting GUS activity in individual lines are described. Methods amplification sequences flanking transposant insertions subsequent molecular...
Stomata are the pores on a leaf surface that regulate gas exchange. Each stoma consists of two guard cells whose movements pore opening and thereby control CO2 fixation water loss. Guard cell depend in part remodeling vacuoles, which have been observed to change from highly fragmented state fused morphology during stomata opening. This requires membrane fusion mechanism responds rapidly environmental signals, allowing plants respond diurnal stress cues. With vacuoles being both large...