A5085477137- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Wheat and Barley Genetics and Pathology
- Genetic and Environmental Crop Studies
- Soybean genetics and cultivation
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Agricultural pest management studies
- Genetics and Plant Breeding
- Plant Micronutrient Interactions and Effects
- Cassava research and cyanide
- Plant Pathogenic Bacteria Studies
- Phytase and its Applications
- Microbial Fuel Cells and Bioremediation
- Plant pathogens and resistance mechanisms
- Agriculture and Biological Studies
- Ammonia Synthesis and Nitrogen Reduction
- Phytoplasmas and Hemiptera pathogens
- Plant responses to water stress
- GABA and Rice Research
Institute of Genetics
2000-2024
Magyar Agrár- és Élettudományi Egyetem
2021-2024
HUN-REN Szegedi Biológiai Kutatóközpont
1998-2024
Institute of Plant Biology
2019-2024
Hungarian Research Network
2021-2023
Research Network (United States)
2023
Agricultural Biotechnology Institute
2007-2019
National Agricultural Research and Innovation Centre
2015-2018
John Innes Centre
2005-2009
Hungarian Academy of Sciences
1996-2008
Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes GRAS protein essential for Nod-factor signaling. functions downstream of Nod-factor-induced calcium spiking and calcium/calmodulin-dependent kinase. We NSP2-GFP expressed constitutive promoter is localized to endoplasmic reticulum/nuclear envelope relocalizes nucleus...
Legumes form symbiotic associations with both mycorrhizal fungi and nitrogen-fixing soil bacteria called rhizobia. Several of the plant genes required for transduction rhizobial signals, Nod factors, are also necessary symbiosis. Here, we describe cloning characterization one such gene from legume Medicago truncatula . The DMI1 (does not make infections) encodes a novel protein low global similarity to ligand-gated cation channel domain archaea. is highly conserved in angiosperms ancestral...
Abstract Rhizobial bacteria activate the formation of nodules on appropriate host legume plant, and this requires bacterial signaling molecule Nod factor. Perception factor in plant leads to activation a number rhizobial-induced genes. Putative transcriptional regulators GRAS family are known function signaling, but these proteins have not been shown be capable direct DNA binding. Here, we identify an ERF transcription factor, Required for Nodulation (ERN), which contains highly conserved...
Abstract A core genetic map of the legume Medicago truncatula has been established by analyzing segregation 288 sequence-characterized markers in an F2 population composed 93 individuals. These molecular correspond to 141 ESTs, 80 BAC end sequence tags, and 67 resistance gene analogs, covering 513 cM. In case EST-based we used intron-targeted marker strategy with primers designed anneal conserved exon regions amplify across intron regions. Polymorphisms were significantly more frequent vs....
Nodulation is tightly regulated in legumes to ensure appropriate levels of nitrogen fixation without excessive depletion carbon reserves. This balance maintained by intimately linking nodulation and its regulation with plant hormones. It has previously been shown that ethylene jasmonic acid (JA) are able regulate Nod factor signal transduction. Here, we characterize the nature abscisic (ABA) nodulation. We show application ABA inhibits nodulation, bacterial infection, nodulin gene expression...
Legumes form endosymbiotic associations with nitrogen-fixing bacteria and arbuscular mycorrhizal (AM) fungi which facilitate nutrient uptake. Both symbiotic interactions require a molecular signal exchange between the plant symbiont, this involves conserved symbiosis (Sym) signaling pathway. In order to identify genes required for intracellular accommodation of AM fungi, we characterized Medicago truncatula mutants defective rhizobial infection nodule cells colonization root by hyphae. Here,...
Significance In certain legume–rhizobia symbioses, the host plant is thought to control terminal differentiation of its bacterial partner leading nitrogen fixation. Medicago truncatula , over 600 genes coding for nodule-specific cysteine-rich (NCR) peptides are expressed during nodule development and have been implicated in bacteroid differentiation. Up now it was generally assumed that most these peptides, if not all, act redundantly. By demonstrating deletion a single member NCR gene...
Legumes engage in root nodule symbioses with nitrogen-fixing soil bacteria known as rhizobia. In cells, are enclosed membrane-bound vesicles called symbiosomes and differentiate into bacteroids that capable of converting atmospheric nitrogen ammonia. Bacteroid differentiation prolonged intracellular survival essential for development functional nodules. However, the Medicago truncatula-Sinorhizobium meliloti symbiosis, incompatibility between symbiotic partners frequently occurs, leading to...
The formation of a nitrogen-fixing nodule requires the coordinated development rhizobial colonization and organogenesis. Based on its mutant phenotype, lumpy infections (lin), LIN functions at an early stage symbiotic process, required for both infection thread growth in root hair cells further primordia. We show that spontaneous nodulation activated by calcium- calmodulin-dependent protein kinase is independent LIN; thus, not necessary From this, we infer predominantly during abortion this...
Abstract Background Recent genome sequencing enables mega-base scale comparisons between related genomes. Comparisons animals, plants, fungi, and bacteria demonstrate extensive synteny tempered by rearrangements. Within the legume plant family, glimpses of have also been observed. Characterizing syntenic relationships in legumes is important transferring knowledge from model to crops that are sources protein, fixed nitrogen, health-promoting compounds. Results We uncovered two large soybean...
Plant systemic signaling pathways allow the integration and coordination of shoot root organ metabolism development at whole-plant level depending on nutrient availability. In legumes, two have been reported in Medicago truncatula model to regulate nitrogen-fixing symbiotic nodulation. Both involve leucine-rich repeat receptor-like kinases acting shoots proposed perceive peptides produced roots soil this study, we characterized M. Jemalong A17 genotype a mutant allelic series affecting...
The symbiotic relationship between legumes and rhizobium bacteria in root nodules has a high demand for iron, questions remain regarding which transporters are involved. Here, we characterize two nodule-specific Vacuolar iron Transporter-Like (VTL) proteins Medicago truncatula. Localization of fluorescent fusion mutant studies were carried out to correlate with existing RNA-seq data showing differential expression VTL4 VTL8 during early late infection, respectively. vtl4 insertion lines...
In the nodules of IRLC legumes, including Medicago truncatula, nitrogen-fixing rhizobia undergo terminal differentiation resulting in elongated and endoreduplicated bacteroids specialized for nitrogen fixation. This irreversible transition is mediated by host produced nodule-specific cysteine-rich (NCR) peptides, which c. 700 are encoded M. truncatula genome but only few them have been proved to be essential We carried out characterization nodulation phenotype three ineffective mutants using...
Abstract Background The formation of functional symbiotic nodules is the result a coordinated developmental program between legumes and rhizobial bacteria. Genetic analyses in have been used to dissect signaling processes required for establishing legume-rhizobial endosymbiotic association. Compared early events interaction, less attention has paid plant loci colonization functioning nodule. Here we describe identification characterization number new genetic Medicago truncatula that are...
Legumes form endosymbiotic interaction with host compatible rhizobia, resulting in the development of nitrogen-fixing root nodules. Within symbiotic nodules, rhizobia are intracellularly accommodated plant-derived membrane compartments, termed symbiosomes. In mature nodule, massively colonized cells tolerate existence without manifestation visible defense responses, indicating suppression plant immunity nodule favur partner.
Symbiotic interactions between legumes and rhizobia lead to the development of root nodules nitrogen fixation by differentiated bacteroids within nodules. Differentiation endosymbionts is reversible or terminal, determined plant effectors. In inverted repeat lacking clade legumes, nodule-specific cysteine-rich (NCR) peptides control terminal differentiation bacteroids. Medicago truncatula contains ∼700 NCR-coding genes. However, role few NCR has been demonstrated. Here, we report...
Legumes have evolved a nitrogen-fixing symbiotic interaction with rhizobia, and this association helps them to cope the limited nitrogen conditions in soil. The compatible between host plant rhizobia leads formation of root nodules, wherein internalization transition into their form, termed bacteroids, occur. Rhizobia nodules Inverted Repeat-Lacking Clade legumes, including Medicago truncatula, undergo terminal differentiation, resulting elongated endoreduplicated bacteroids. This...
The bs5 resistance gene against bacterial spot was identified by map-based cloning. recessive of pepper (Capsicum annuum L.) conditions a non-hypersensitive trait, characterized slightly swollen, pale green, photosynthetically active leaf tissue, following Xanthomonas euvesicatoria infection. isolation the cloning revealed that protein shorter 2 amino acids as compared to wild type Bs5 protein. natural acid deletion occurred in cysteine-rich transmembrane domain tail-anchored (TA) protein,...
Nuclear magnetic resonance (NMR) spectroscopy profiling was used to provide an unbiased assessment of changes the metabolite composition seeds and define genetic variation for a range pea seed metabolites. Mature from recombinant inbred lines, derived three mapping populations which there is substantial marker linkage information, were grown in two environments/years analysed by non-targeted NMR. Adaptive binning NMR data, followed analysis quantitative among lines individual bins,...
To demonstrate the importance of an extensively studied early nodulin gene ENOD12 in symbiotic nodule development, plants different Medicago sativa subspecies were tested for presence or absence alleles. In M. s. ssp coerulea w2 (Mcw2), two genes detected, whereas quasifalcata k93 (Mqk93) only one was present. both plants, expressed nodules induced by Rhizobium meliloti. The nucleotide sequence showed that Mcw2-specific similar to ENOD12A and ENOD12B tetraploid sativa. from Mqk93...