A5018152023- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Hemoglobin structure and function
- Renal Transplantation Outcomes and Treatments
- RNA modifications and cancer
- Nicotinic Acetylcholine Receptors Study
- Transplantation: Methods and Outcomes
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Ion Transport and Channel Regulation
- Metalloenzymes and iron-sulfur proteins
- Endoplasmic Reticulum Stress and Disease
- Neuroscience of respiration and sleep
- Stress Responses and Cortisol
- Gene expression and cancer classification
- Microfluidic and Capillary Electrophoresis Applications
- Cholinesterase and Neurodegenerative Diseases
- Lipid Membrane Structure and Behavior
- Genomics and Chromatin Dynamics
- Cardiomyopathy and Myosin Studies
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
Agilent Technologies (United States)
2018-2025
Yale University
2009-2018
W. M. Keck Foundation
2008-2018
Washington University in St. Louis
2015
National Heart Lung and Blood Institute
2009
University of Georgia
1996-2000
Florida College
1999
Duke University Hospital
1999
University of Florida
1999
Duke Medical Center
1999
Tourette's syndrome is a common developmental neuropsychiatric disorder characterized by chronic motor and vocal tics. Despite strong genetic contribution, inheritance complex, risk alleles have proven difficult to identify. Here, we describe an analysis of linkage in two-generation pedigree leading the identification rare functional mutation HDC gene encoding L-histidine decarboxylase, rate-limiting enzyme histamine biosynthesis. Our findings, together with previously published data from...
Virions are thought to contain all the essential proteins that govern virus egress from host cell and initiation of replication in target cell. It has been known for some time influenza virions nine viral proteins; however, analyses other enveloped viruses have revealed can also be detected virions. To address whether same is true virus, we used two complementary mass spectrometry approaches perform a comprehensive proteomic analysis purified particles. In addition aforementioned...
Panorama is a web application for storing, sharing, analyzing, and reusing targeted assays created refined with Skyline,1 an increasingly popular Windows client software tool proteomics experiments. allows laboratories to store organize curated results contained in Skyline documents fine-grained permissions, which facilitates distributed collaboration secure sharing of published unpublished data via web-browser interface. It fully integrated the workflow supports publishing document directly...
The widespread use of mass spectrometry for protein identification has created a demand computationally efficient methods matching data to databases. A search using X!Tandem, popular and representative program, can require hours or days complete, particularly when missed cleavages post-translational modifications are considered. Existing techniques accelerating X!Tandem by employing parallelism unsatisfactory variety reasons. paper describes parallelization called X!!Tandem, that shows...
Early life neglect is an important public health problem which can lead to lasting psychological dysfunction. Good animal models are necessary understand the mechanisms responsible for behavioral and anatomical pathology that results. We recently described a novel model of early neglect, Maternal Separation with Weaning (MSEW), produces changes in mouse persist into adulthood. To begin mechanism by MSEW leads these we applied cDNA microarray, next-generation RNA sequencing (RNA-seq),...
Detection of differentially abundant proteins in label-free quantitative shotgun liquid chromatography-tandem mass spectrometry (LC-MS/MS) experiments requires a series computational steps that identify and quantify LC-MS features. It also statistical analyses distinguish systematic changes abundance between conditions from artifacts biological technical variation. The 2015 study the Proteome Informatics Research Group (iPRG) Association Biomolecular Resource Facilities (ABRF) aimed to...
The zinc and cobalt forms of the prototypic γ-carbonic anhydrase from Methanosarcina thermophila were characterized by extended X-ray absorption fine structure (EXAFS) kinetics investigated using steady-state spectrophotometric 18O exchange equilibrium assays. EXAFS results indicate that isomorphously replaces metals coordinate three histidines two or water molecules. efficiency either Zn−Cam Co−Cam for CO2 hydration (kcat/Km) was severalfold greater than HCO3- dehydration at physiological...
Immune tolerance to transplanted organs is impaired when the innate immune system activated in response tissue necrosis that occurs during harvesting and implantation procedures. A key molecule this pathway intracellular TLR signal adaptor known as myeloid differentiation primary gene 88 (MyD88). After transplantation, MyD88 induces DC maturation well production of inflammatory mediators, such IL-6 TNF-α. However, upstream activators function transplantation have not been identified. Here,...
Conflicting reports are available with regard to the effects of childhood abuse and neglect on hippocampal function in children. While earlier imaging studies some animal work have suggested that early-life stress (ELS) manifest only adulthood, more recent documented impaired maltreated children adolescents. Additional using modes is needed clarify ELS development. In this regard, genomic, proteomic, molecular tools uniquely mouse make it a particularly attractive model system study issue....
Successful addiction treatment depends on maintaining long-term abstinence, making relapse prevention an essential therapeutic goal. However, exposure to environmental cues associated with drug use often thwarts abstinence efforts by triggering using memories that drive craving and relapse. We sought develop a dual approach for weakening cocaine through phosphoproteomic identification of targets regulated in opposite directions memory extinction compared reconsolidation male Sprague-Dawley...
X-ray absorption spectroscopy at the molybdenum and selenium K-edges has been used to probe active site structure of Escherichia coli formate dehydrogenase H. The sites both oxidized reduced wild-type protein, a variant containing cysteine instead selenocysteine, were studied. enzymes found be very similar, novel des-oxo site, with four Mo−S ligands 2.35 Å, (probably) one Mo−O 2.1 Mo−Se ligand 2.62 Å being indicated from Mo K-edge data. EXAFS not only is in good agreement data but also...
We report the application of matrix-assisted laser desorption ionization (MALDI) to monitor recombinant protein expression in whole bacteria. This technique is characterized by rapid sample preparation that provides analysis samples extracted directly from growth media less than 10 min. The mass spectrometric method holds several advantages over gel electrophoresis, conventional for examining content cells. Comparisons between two methods are presented terms increased speed, efficiency,...
Caveolae are organelles abundant in the plasma membrane of many specialized cells including endothelial (ECs), epithelial cells, and adipocytes, these caveolin-1 (Cav-1) is major coat protein essential for formation caveolae. To identify proteins that require Cav-1 stable incorporation into raft domains, a quantitative proteomics analysis using isobaric tagging relative absolute quantification was performed on rafts isolated from wild-type Cav-1-deficient mice. In three independent...
While most oncologists know that many chemotherapies are dosed on a body surface area (BSA) basis, fewer the equations used to estimate BSA based study done over 100 years ago using data from just 9 people. Moreover, two patients with same can have factors, such as composition and genetics, which impact drug’s pharmacokinetics pharmacodynamics, resulting in worse therapeutic outcomes. To solve this problem, we developed medical device personalize dose of chemotherapy patient during an...
Proteomics is a rapidly transforming interdisciplinary field of research that embraces diverse set analytical approaches to tackle problems in fundamental and applied biology. This viewpoint article highlights the benefits interlaboratory studies standardization initiatives enable investigators address many challenges found proteomics research. Among these initiatives, we discuss our efforts on comprehensive performance standard for characterizing PTMs by MS was recently developed...
ARPP-16, ARPP-19, and ENSA are inhibitors of protein phosphatase PP2A. ARPP-19 phosphorylated by Greatwall kinase inhibit PP2A during mitosis. ARPP-16 is expressed in striatal neurons where basal phosphorylation MAST3 inhibits regulates key components signaling. The ARPP-16/19 proteins were discovered as substrates for PKA, but the function PKA unknown. We find that or mutually suppresses ability other to act on ARPP-16. Phosphorylation also acts prevent inhibition MAST3. Moreover,...
X-ray absorption spectroscopy has been used to define the copper-site structures in both resting (oxidized) and dithionite-reduced states of amine oxidases from bovine plasma, porcine kidney, pea seedlings, gram-positive bacterium Arthrobacter P1. The Cu(II) EXAFS data are consistent with four first-shell N,O scatterers (three which imidazoles) at a distance 1.98−2.00 Å for all five oxidases. These assigned as equatorial ligands. Because raw essentially identical enzymes examined,...
We have developed an integrated web-accessible software system called the Yale Protein Expression Database (YPED) to address need for storage, retrieval, and analysis of large amounts data from high throughput proteomic technologies. YPED is open source which integrates gel results with protein identifications DIGE experiments. The associates spots image, analyzed DeCyder, mass spectrometric selected spots. Following in trypsin digestion, proteins interest are using MALDI-TOF/TOF on AB 4700...