A5101505972- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Sarcoma Diagnosis and Treatment
- Liver physiology and pathology
- Animal Nutrition and Physiology
- CAR-T cell therapy research
- Protein Tyrosine Phosphatases
- Tumors and Oncological Cases
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Pancreatic function and diabetes
- Phagocytosis and Immune Regulation
- Liver Disease Diagnosis and Treatment
- Effects and risks of endocrine disrupting chemicals
- Galectins and Cancer Biology
- Child Nutrition and Water Access
- Infant Nutrition and Health
- Advanced Fluorescence Microscopy Techniques
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Erythrocyte Function and Pathophysiology
- Animal testing and alternatives
Harvard Stem Cell Institute
2019-2024
Massachusetts General Hospital
2019-2024
Center for Cancer Research
2022-2024
National University of Singapore
2012-2024
Agency for Science, Technology and Research
2024
Institute of Molecular and Cell Biology
2024
University of Jinan
2024
China University of Petroleum, East China
2022
Harvard University
2003-2021
Sichuan Agricultural University
2015-2020
Bisphenol-A is an important environmental contaminant due to the increased early-life exposure that may pose significant health-risks various organisms including humans. This study aimed use zebrafish as a toxicogenomic model capture transcriptomic and phenotypic changes for inference of signaling pathways, biological processes, physiological systems identify potential biomarker genes are affected by bisphenol-A. Phenotypic analysis using wild-type larvae revealed BPA toxicity caused cardiac...
Chronic inflammation is a major etiological factor for hepatocellular carcinoma (HCC), but how immune cells respond in the initiation of hepatocarcinogenesis remains uncharacterized. This study aims to investigate response and roles neutrophils early hepatocarcinogenesis.By inducible expression oncogenic kras(V12) hepatocytes transgenic zebrafish combined with live imaging transparent larvae, liver was characterized their investigated by pharmaceutical genetic manipulations.We found rapid...
Myc is a pleiotropic transcription factor that involved in many cellular activities relevant to carcinogenesis, including hepatocarcinogenesis. The zebrafish has been increasingly used model human diseases and it particularly valuable helping identify common conserved molecular mechanisms vertebrates. Here we generated liver tumor transgenic by liver-specific expression of mouse using Tet-On system. Dosage-dependent induction specifically the was observed our zebrafish, TO(Myc), elevated...
Hepatocellular carcinoma (HCC) occurs more frequently and aggressively in men than women, but the mechanistic basis of this gender disparity is obscure. Chronic inflammation a major etiologic factor HCC, so we investigated role cortisol discrepancy zebrafish model HCC. Inducible expression oncogenic KrasV12 hepatocytes transgenic resulted accelerated liver tumor progression males. These tumors were heavily infiltrated with tumor-associated neutrophils (TAN) macrophages (TAM) versus females,...
Postnatal rapid growth by excess intake of nutrients has been associated with an increased susceptibility to diseases in neonates intra-uterine restricted (IUGR). The aim the present study was determine whether postnatal nutritional restriction could improve intestinal development and immune function IUGR using piglets as model. A total twelve pairs normal-birth weight (NBW) (7 d old) were randomly assigned receive adequate nutrient or (RNI) artificially liquid feeding for a period 21 d....
The current study aimed to determine whether dietary nucleotides supplementation could improve growth performance, intestinal development and immune function of intra-uterine restricted (IUGR) neonate using pig as animal model. A total 14 pairs normal birth weight (NBW) IUGR piglets (7 days old) were randomly assigned receive a milk-based control diet (CON diet) or supplemented with (NT for period 21 days. Blood samples, tissues digesta collected at necropsy analyzed morphology, digestive...
Gain-of-function mutations in the protein-tyrosine phosphatase SHP2 are most frequently occurring sporadic juvenile myelomonocytic leukemia (JMML) and JMML-like myeloproliferative neoplasm (MPN) associated with Noonan syndrome (NS). Hematopoietic stem progenitor cells (HSPCs) disease propagating of JMML. Here, we explored transcriptomes HSPCs derived from JMML patients a novel NS zebrafish model. In addition to major traits, CRISPR/Cas9 knock-in Shp2D61G mutant recapitulated MPN phenotype,...
In 2006, the European Union (EU) has decided to forbid use of antibiotics as growth promoters. Although many researches had been conducted about fiber source alternatives antibiotics, there are still lack reports in literature optimum level sugar beet pulp supplementation, affecting performance and nutrient digestibility weaning pigs. Therefore, different was added diets determine effects supplementation on performance, digestibility, fecal microflora, blood profile incidence diarrhea A...
type 2 diabetes mellitus (T2DM) is a complicated disease that can affect bone health, but the change in biochemical markers caused by T2DM was controversial, so aim of this study to investigate whether there discrepancy levels between postmenopausal women with and non-diabetic explore relationship level glycosylated hemoglobin A1c (HbA1c) these subjects. A total 237 diabetic visiting First Affiliated Hospital Anhui Medical University from January 2017 October 2018 93 healthy were...
Abstract We have previously demonstrated the pro-tumoral role of neutrophils using a kras -induced zebrafish hepatocarcinogenesis model. To further illustrate molecular basis role, Tumor-associated (TANs) were isolated by fluorescence-activated cell sorting (FACS) and transcriptomic analyses carried out RNA-Seq. Differentially expressed gene profiles TANs from larvae, male female livers indicate great variations during liver tumorigenesis, but common responsive canonical pathways included an...
Thermal crack induced by the hydration heat of massive concrete is main problem that needs to be strictly controlled during pouring process concrete. In this study, pier and its influencing factors are studied numerically. The stress caused analytically derived. influences adiabatic temperature rise, ambient convection coefficient between outer surface environment on studied. results show peak values increase with maximum reaction rate temperature. value reduces decrease because dissipation...
Activation of hepatic stellate cells (HSC) plays a crucial role in the liver disease progression from fibrosis/cirrhosis to cancer. Here, we found rapid change microenvironment after kras V12 -induction zebrafish with progressively increased stromal cell number and enlarged size. Neutrophils macrophages exhibited faster response than HSCs. By manipulating numbers neutrophils through morpholino knockdown, that contributed both HSC survival activation while appear be only required for...
Liver cancers including both hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) have increased steadily with the prevalence of non-alcoholic steatohepatitis (NASH), but underlying mechanism for transition from NASH to liver remains unclear. Here we first employed diet-induced zebrafish found that elevated level satiety hormone, leptin, induced overexpression tgfb1. Then developed tgfb1a transgenic inducible, hepatocyte-specific expression. Interestingly, chronically high induction...
Cancer cachexia affects up to 80% of the patients with advanced solid cancer and leads excessive muscle wasting. Here, using an inducible zebrafish HCC model driven by oncogenic krasG12V, we observed a progressive wasting phenotype in adult zebrafish, characterized significant loss body weight fibers. By differential feeding, that overfeeding caused fatty liver, accelerated carcinogenesis Interestingly, leptin, obesity hormone, was upregulated hepatocytes groups. We also found progressively...
T cell immunotherapies have revolutionized treatment for a subset of cancers. Yet, major hurdle has been the lack facile and predicative preclinical animal models that permit dynamic visualization immune responses at single-cell resolution in vivo. Here, optically clear immunocompromised zebrafish were engrafted with fluorescent-labeled human cancers along chimeric antigen receptor (CAR T) cells, bispecific engagers (BiTEs), antibody peptide epitope conjugates (APECs), allowing real-time...