A5031226363- RNA Research and Splicing
- Nuclear Structure and Function
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- DNA Repair Mechanisms
- Muscle Physiology and Disorders
- Fungal and yeast genetics research
- Cancer therapeutics and mechanisms
- DNA and Nucleic Acid Chemistry
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Probiotics and Fermented Foods
- Carcinogens and Genotoxicity Assessment
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
- Health and Medical Research Impacts
- Biotin and Related Studies
- RNA Interference and Gene Delivery
Emory University
2016-2025
Emory and Henry College
2023-2025
Florida State University
2024-2025
University of Chicago
2016
Graduate School USA
2016
Weatherford College
1993-2016
University of Nicosia
2016
Oglethorpe University
2015
AID Atlanta
2012
Akita Industrial Technology Center
2010
The regulated process of protein import into the nucleus a eukaryotic cell is mediated by specific nuclear localization signals (NLSs) that are recognized receptors. This study seeks to decipher energetic details NLS recognition receptor importin α through quantitative analysis variant NLSs. relative importance each residue in two monopartite sequences was determined using an alanine scanning approach. These measurements yield definition sequence where required lysine followed other basic...
From transcription to translation, mRNA is complexed with heterogeneous nuclear ribonucleoproteins (hnRNP proteins) that mediate processing, export from the nucleus, and delivery into cytoplasm. Although mechanism unknown, of mature nucleus a critical regulatory step in gene expression. Analyses hnRNP proteins have shown many these are required for this essential cellular process. In study, we characterize Saccharomyces cerevisiae Nab2 protein, which was first identified as poly(A)...
The evolutionarily conserved mRNA export receptor Mex67/NXF1 associates with mRNAs through its adaptor, Yra1/REF, allowing ribonucleoprotein (mRNP) exit nuclear pores. However, alternate adaptors should exist, since Yra1 is dispensable for in Drosophila and Caenorhabditis elegans . Here we report that Mex67 interacts directly Nab2, an essential shuttling mRNA-binding protein required export. We further show enhances the interaction between Nab2 Mex67, becomes cells overexpressing or Mex67....
Abstract Sequencing of human patient tumors has identified recurrent missense mutations in genes encoding core histones. We report that convert histone H3 amino acid 50 from a glutamate to lysine (H3E50K) support an oncogenic phenotype. Expression H3E50K is sufficient transform cells as evidenced by increase cell migration and invasion, proliferation clonogenicity. also increases the invasive phenotype context co-occurring BRAF mutations, which are present characterized H3E50K. H3E50 lies on...
The Saccharomyces cerevisiae gene, RNA1, encodes a protein with extensive homology to the mammalian Ran/TC4 GTPase activating protein. Using indirect immunofluorescence microscopy, we have demonstrated that rna1-1 mutant cells are defective in nuclear import of several proteins. same result is obtained when examined living using fused naturally green fluorescent These findings suggest role for Rna1p trafficking proteins across membrane. To investigate this more directly, an vitro assay...
RNA-binding proteins have been suggested to move in association with RNA as it leaves the nucleus. The NPL3 gene of yeast Saccharomyces cerevisiae encodes nuclear protein consensus motifs and similarity heterogeneous ribonucleoproteins members S/R family. We show that although Npl3 is located nucleus, can shuttle between nuclei heterokaryons. In contrast, other nucleus-targeted do not leave nucleus under similar conditions. Mutants missing or N terminus are still capable shuttling out...
For mRNA to be transported from the nucleus cytoplasm, it must travel site of transcription through nuclear interior pore. Studies in Saccharomyces cerevisiae have suggested a relationship between poly(A) RNA trafficking and myosin-like protein 1 (Mlp1p), nuclear-pore associated that is homologous mammalian Tpr (translocated promoter region) [Kosova, B., Panté, N., Rollenhagen, C., Podtelejnikov, A., Mann, M., Aebi, U., Hurt, E. (2000) J. Biol. Chem . 275, 343–350]. We identified yeast...
Many important regulatory proteins, including cell cycle regulators and transcription factors, contain a phosphorylation site within or adjacent to classic nuclear localization signal (NLS) sequence. Previous studies show that the of these cargoes can be regulated by at sites. It was hypothesized this regulates import NLS cargo proteins modulating interaction with transport receptor, importin α. In study, we utilize in vitro solution binding assays vivo analyses directly test model. We...
Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of inner basket mediate interactions these active genes. To investigate physical link between NPC loci, we identified interact carboxyl-terminal globular domain by tandem affinity purification coupled mass spectrometry. This analysis led to identification several...
Characterization of the interactions between soluble factors required for nuclear transport is key to understanding process trafficking. Using a synthetic lethal screen with rna1-1 strain, we have identified genetic interaction Rna1p, GTPase activating protein transport, and yeast importin-beta, component localization signal receptor. By use fusion proteins, demonstrate that Rna1p physically interacts importin-beta. Mutants in importin-beta exhibit vivo import defects, localizes envelope...
The nuclear poly(A)-binding protein 1 (PABPN1) is a ubiquitously expressed that plays critical role in polyadenylation. Short expansions of the polyalanine tract N-terminus PABPN1 lead to oculopharyngeal muscular dystrophy (OPMD), which an adult onset disease characterized by eyelid drooping, difficulty swallowing and weakness proximal limb muscles. Although significant data from vitro biochemical assays define function control poly(A) tail length, little known about mammalian cells. To...