A5048955091- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Aluminum toxicity and tolerance in plants and animals
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Potato Plant Research
- Genomics and Phylogenetic Studies
- Polymer-Based Agricultural Enhancements
- Photosynthetic Processes and Mechanisms
- Phytase and its Applications
- Soil Carbon and Nitrogen Dynamics
- Trace Elements in Health
- Microbial Community Ecology and Physiology
- Mass Spectrometry Techniques and Applications
- Magnetic and Electromagnetic Effects
- Bioinformatics and Genomic Networks
- Wastewater Treatment and Nitrogen Removal
- Machine Learning in Bioinformatics
- Genetic diversity and population structure
- Plant Reproductive Biology
Forschungszentrum Jülich
2016-2024
University of Liège
2016-2019
Fund for Scientific Research
2016
Cluster of Excellence on Plant Sciences
2014
Heinrich Heine University Düsseldorf
2014
Max Planck Institute of Molecular Plant Physiology
2010-2012
Max Planck Society
2010-2012
Solanum pennellii is a wild tomato species endemic to Andean regions in South America, where it has evolved thrive arid habitats. Because of its extreme stress tolerance and unusual morphology, an important donor germplasm for the cultivated lycopersicum. Introgression lines (ILs) which large genomic S. lycopersicum are replaced with corresponding segments from can show remarkably superior agronomic performance. Here we describe high-quality genome assembly parents IL population. By...
Abstract Here, we characterize a plastidial thioredoxin (TRX) isoform from Arabidopsis thaliana that defines previously unknown branch of TRXs lying between x- and y-type thus was named TRX z. An knockout mutant z had severe albino phenotype inhibited in chloroplast development. Quantitative real-time RT-PCR analysis the suggested expressions genes depend on plastid-encoded RNA polymerase (PEP) were specifically decreased. Similar results obtained upon virus-induced gene silencing (VIGS)...
Next-generation sequencing has triggered an explosion of available genomic and transcriptomic resources in the plant sciences. Although genome transcriptome become orders magnitudes cheaper more efficient, often functional annotation process is lagging behind. This might be hampered by lack a comprehensive enumeration simple-to-use tools to researcher. In this review, we present (i) typical ontologies used sciences, (ii) useful databases for annotation, (iii) what expect from annotated...
There is a dynamic reciprocity between plants and their environment: soil physiochemical properties influence plant morphology metabolism, root exudates shape the environment surrounding roots. Here, we investigate reproducibility of trait changes in response to three growth environments. We utilized fabricated ecosystem (EcoFAB) devices grow model grass Brachypodium distachyon distinct media across four laboratories: phosphate-sufficient -deficient mineral allowed assessment effects...
The global need for increased food production means that agriculture is moving into regions with lower rainfall and saline soils, which occupy over 6% of the world land area (Munns, 2005). aim this workshop was to work towards completing an energy budget mechanisms salinity tolerance in crop plants, as a guide most cost-effective breeding strategies increasing salt yield important crops. discussions took account supply from mitochondria chloroplasts, demands transport water, Na+, Cl− K+,...
Pseudomonas spp. make up 1.6% of the bacteria in soil and are found throughout world. More than 140 species this genus have been identified, some beneficial to plant. Several family Pseudomonadaceae, including Azotobacter vinelandii AvOP, stutzeri A1501, DSM4166, szotifigens 6HT33bT, sp. strain K1 can fix nitrogen from air. The genes required for these reactions organized a fixation island, obtained via horizontal gene transfer Klebsiella pneumoniae, stutzeri, vinelandii. Today, island is...
Pathogens resistant to classical control strategies pose a significant threat crop yield, with seeds being major transmission route. Bacteriophages, viruses targeting bacteria, offer an environmentally sustainable biocontrol solution. In this study, we isolated and characterized two novel phages, Athelas Alfirin, which infect Pseudomonas syringae Agrobacterium fabrum, respectively, included the recently published Pfeifenkraut phage infecting Xanthomonas translucens. Using simple immersion...
Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal to N-fixing dynamic, depending on supply time. To quantify the dynamics, gnotobiotic, fabricated ecosystem (EcoFAB) was adapted analyse mass balance, image shoot root growth, measure gene expression Brachypodium distachyon inoculated with bacterium...
Bacteriophages are increasingly recognized as key players in modulating plant-microbe interactions, including their potential the biocontrol of plant pathogenic bacteria. In this study, we investigated tripartite interaction between Arabidopsis thaliana, bacterial pathogen Xanthomonas campestris pv. (Xcc), and lytic phage Seregon. Using parallel transcriptomic profiling, characterized host responses during infection treatment. While treatment with Seregon did not lead to eradication Xcc, it...
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As the most studied post-translational modification protein phosphorylation is part of a growing number proteomic studies. But these approaches generate large datasets from which, specific spectrum based data can be hard to find. In 2007 PhosPhAt database made it its goal collect and present Arabidopsis for scientific community. At present, 3.0 consolidates phosphoproteomics 19 published 5460 Unique phosphoproteins are listed, about 25% which have been identified in at least two independent...
This study reports the comprehensive comparison of 15N metabolic labeling and label free proteomic strategies for quantitation, with particular focus on plant proteomics. Our investigation proteome coverage, dynamic range quantitative precision a wide mixing ratios protein loadings aim to aid investigators in decision making process during experimental design. One main characteristics strategy is applicability all starting material, which limitation labeling. However, particularly at up...
Abstract A non-invasive plant phenotyping platform, GrowScreen-PaGe , was used to resolve the dynamics of shoot and root growth model cereal Brachypodium ( distachyon Bd21-3) in response promoting (PGP) bacteria Azospirillum brasilense Sp245). Inoculated plants had greater early vigor higher P use efficiency than non-inoculated at low temperature conditions. Root systems were imaged non-invasively eight time points data combined with leaf area, biomass nutrient content from destructive...
The potential of plant growth promoting (PGP) bacteria in improving the performance plants suboptimal environments is increasingly acknowledged, but little information available on mechanisms underlying this interaction, particularly when are subjected to a combination stresses. In study, we investigated effects inoculation with PGP Azospirillum brasilense (Azospirillum) metabolism model cereal Brachypodium distachyon (Brachypodium) grown at low temperatures and supplied insufficient...
High temperatures inhibit plant growth. A proposed strategy for improving productivity under elevated is the use of growth-promoting rhizobacteria (PGPR). While effects PGPR on shoots have been extensively explored, roots-particularly their spatial and temporal dynamics-have hard to study, due below-ground nature. Here, we characterized time- tissue-specific morphological changes in bacterized plants using a novel non-invasive high-resolution phenotyping imaging platform-GrowScreen-Agar II....
Summary Regulation of plant Zn acquisition is poorly understood, while deficiency affects over 2 billion people worldwide. We therefore dissected the dynamic response to changes in supply Arabidopsis. Hydroponically-grown starved plants were re-supplied with Zn. Subsequent time-resolved sampling strategy allowed concomitant quantification dynamics uptake, microsomal and soluble proteins, specific transcripts, space (roots shoots) time. accumulates roots within 10min, but 8h are needed before...
Abstract The biological processes underlying zinc homeostasis are targets for genetic improvement of crops to counter human malnutrition. Detailed phenotyping, ionomic, RNA‐Seq analyses and flux measurements with 67 Zn isotope revealed whole‐plant molecular events upon varying supply during resupply starved Brachypodium distachyon (Brachypodium) plants. Although both deficiency excess hindered growth, accumulation biomass micronutrients into roots shoots differed depending on supply....