A5056351366- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Horticultural and Viticultural Research
- Plant responses to water stress
- Plant Water Relations and Carbon Dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Fermentation and Sensory Analysis
- Aluminum toxicity and tolerance in plants and animals
- Ion Transport and Channel Regulation
- Plant and Biological Electrophysiology Studies
- Plant Molecular Biology Research
- Plant Physiology and Cultivation Studies
- GABA and Rice Research
- Magnetic and Electromagnetic Effects
- Membrane-based Ion Separation Techniques
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Ion channel regulation and function
- Leaf Properties and Growth Measurement
- Postharvest Quality and Shelf Life Management
- Remote Sensing in Agriculture
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Magnesium in Health and Disease
The University of Adelaide
2016-2025
Australian Research Council
2015-2024
Australian Wine Research Institute
2012-2024
ARC Centre of Excellence in Plant Energy Biology
2014-2023
Charles Sturt University
2021-2022
Wine Australia
2006-2022
Plant Industry
1991-2012
ACT Government
2004-2012
Cooperative Research Centre for Tissue Growth and Repair
2004-2012
The University of Western Australia
2009-2011
There is strong evidence that aquaporins are central components in plant water relations. Plant species possess more aquaporin genes than from other kingdoms. According to sequence similarities, four major groups have been identified, which can be further divided into subgroups may correspond localization and transport selectivity. They involved compatible solute distribution, gas-transfer (CO2, NH3) as well micronutrient uptake (boric acid). Recent advances determining the structure of some...
Abstract We report physiological and anatomical characteristics of water transport across roots grown in soil two cultivars grapevine (Vitis vinifera) differing response to stress (Grenache, isohydric; Chardonnay, anisohydric). Both have similar root hydraulic conductances (L o; normalized dry weight) that change diurnally. There is a positive correlation between L o transpiration. Under stress, both reduced minimum daily (predawn) attributed the development apoplastic barriers....
Over the last decade, considerable advances have occurred in understanding molecular biology and biophysics of water permeation across plant membranes tissues. Spurred on by rapid cloning functional characterization a superfamily major intrinsic proteins, some which function as aquaporins, transport small non-electrolytes is being re-examined based proposed these membrane-integral proteins their native membranes. This review focuses number issues that are central to an aquaporin function:...
Abstract The non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues response to biotic and abiotic stress, regulates growth. Until now it was not known whether GABA exerts its effects plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux aluminium-activated malate transporter (ALMT) proteins is activated by anions negatively regulated GABA. Site-directed mutagenesis selected...
Abstract The physiological role and mechanism of nutrient storage within vacuoles specific cell types is poorly understood. Transcript profiles from Arabidopsis thaliana leaf cells differing in calcium concentration ([Ca], epidermis <10 mM versus mesophyll >60 mM) were compared using a microarray screen single-cell quantitative PCR. Three tonoplast-localized Ca2+ transporters, CAX1 (Ca2+/H+-antiporter), ACA4, ACA11 (Ca2+-ATPases), identified as preferentially expressed Ca-rich...
Abstract Background Hydroponic growth systems are a convenient platform for studying whole plant physiology. However, we found through trialling as they described in the literature that our experiments were frequently confounded by factors affected growth, including algal contamination and hypoxia. We also way which plants grown made them poorly amenable to number of common physiological assays. Results The drivers development this hydroponic system were: 1) exclusion light from solution; 2)...
The aquaporin AtPIP2;1 is an abundant plasma membrane intrinsic protein in Arabidopsis thaliana that implicated stomatal closure, and highly expressed membranes of root epidermal cells. When Xenopus laevis oocytes, increased water permeability induced a non-selective cation conductance mainly associated with Na+ . A mutation the pore, G103W, prevented both ionic PIP2;1. Co-expression AtPIP1;2 but abolished conductance. AtPIP2;2 (93% identical to AtPIP2;1) similarly not was inhibited by...
Chloroplast retrograde signaling networks are vital for chloroplast biogenesis, operation, and signaling, including excess light drought stress signaling. To date, has been considered in the context of land plant adaptation, but not regarding origin evolution cascades linking function to stomatal regulation. We show that key elements process, nucleotide phosphatase (SAL1) 3'-phosphoadenosine-5'-phosphate (PAP) metabolism, evolved streptophyte algae-the algal ancestors plants. discover an...
We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these include the need better understand how plants detect water availability, temperature, salinity, rising carbon dioxide (CO2) levels; environmental signals interface endogenous signaling development (e.g. circadian clock flowering time); this integrated controls downstream responses stomatal regulation,...
Silver and gold compounds were tested as potential inhibitors of aquaporins plant‐ human origin. AgNO 3 or silver sulfadiazine inhibited with high potency (EC 50 1–10 μM) the water permeability peribacteroid membrane from soybean (containing Nodulin 26), plasma roots integral proteins), red cells aquaporin 1). Gold HAuCl 4 was less effective but still =10 μM). are more potent than presently widely used mercury containing compounds.
To assess the extent of water flow through channels in membranes intact higher plant cells, effects HgCl2 on hydraulic conductivity (LP) wheat (Triticum aestivum L.) root cells were investigated using a pressure probe. The LP was reduced by 75% presence 100 &mgr;M HgCl2. K+-channel blocker tetraethylammonium had no effect at concentrations that normally block K+ channels. rapidly depolarized membrane potential (Vm) cells. dose-response relationship inhibition and depolarization Vm not...
Abstract The contrasting hydraulic properties of wheat (Triticum aestivum), narrow-leafed lupin (Lupinus angustifolius), and yellow luteus) roots were identified by integrating measurements water flow across different structural levels organization with anatomy modeling. Anatomy played a major role in root hydraulics, influencing axial conductance (L ax) the distribution uptake along root, more localized for aquaporins (AQPs). Lupin had greater L ax than roots, due to xylem development. r)...
We describe an anion channel in the plasmalemma of protoplasts isolated from wheat (Triticum aestivum L.) roots that is activated by aluminum (Al3+). In whole-cell configuration, addition 20-50 microM AlCl3 to external solution depolarized membrane and inward current could remain active for more than 60 min. The activation Al3+ was rapid 20% examined, whereas another 30% a delay 10 min occurred after added. Once activated, changing Cl- concentration shifted reversal potential with ECl,...