A5003756579- High Altitude and Hypoxia
- Erythropoietin and Anemia Treatment
- Neuroscience of respiration and sleep
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Retinal Development and Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Retinal Diseases and Treatments
- Cancer, Hypoxia, and Metabolism
- Blood transfusion and management
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Heart Rate Variability and Autonomic Control
- Glaucoma and retinal disorders
- scientometrics and bibliometrics research
- Angiogenesis and VEGF in Cancer
- Pulmonary Hypertension Research and Treatments
- Cell Adhesion Molecules Research
- Inhalation and Respiratory Drug Delivery
- Sleep and Wakefulness Research
- Connexins and lens biology
- Corneal Surgery and Treatments
- Connective tissue disorders research
- Dietary Effects on Health
- melanin and skin pigmentation
- Hemoglobinopathies and Related Disorders
Inserm
1999-2025
Sorbonne Université
1999-2025
Université Sorbonne Paris Nord
2013-2022
Hypoxie et Poumon
2017-2022
Université Paris Cité
2009-2019
University of Florida
2014-2019
Sorbonne Paris Cité
2012-2019
Centre de Recherche des Cordeliers
2019
Florida Museum of Natural History
2015-2019
Délégation Paris 5
2001-2006
Chloroplast retrograde signaling networks are vital for chloroplast biogenesis, operation, and signaling, including excess light drought stress signaling. To date, has been considered in the context of land plant adaptation, but not regarding origin evolution cascades linking function to stomatal regulation. We show that key elements process, nucleotide phosphatase (SAL1) 3'-phosphoadenosine-5'-phosphate (PAP) metabolism, evolved streptophyte algae-the algal ancestors plants. discover an...
Endoplasmic Reticulum (ER) stress of alveolar epithelial cells (AECs) is recognized as a key event cell dysfunction in pulmonary fibrosis (PF). However, the mechanisms leading to AECs ER and ensuing unfolded protein response (UPR) pathways idiopathic PF (IPF) remain unclear. We hypothesized that hypoxic microenvironment would generate apoptosis through hypoxia-inducible factor-1α (HIF-1α). Combining ex vivo, vivo vitro experiments, we investigated effects hypoxia on UPR stress-mediated...
Abstract Apoptosis plays a major role in the development of central nervous system. Previous studies apoptosis induction during retinal are difficult to interpret, however, because they explored different mouse strains, developmental periods, and used assays. Here, we first established comprehensive sequential pattern cell death whole C57BL/6J retina, from E10.5 postnatal day (P) 21 by using terminal deoxynucleotidyl transferase (TdT) ‐mediated deoxyuridine triphosphate (dUTP)–biotinylated...
The long interspersed element-1 (LINE-1 or L1) retrotransposition has altered the human genome in many ways. In particular, recent vitro studies have demonstrated that retrotranspositional insertion of L1 elements resulted significant genomic deletions. Here we provide evidence for its operation by identifying a approximately 46-kb pathological deletion PDHX gene directly linked to full-length element, patient with pyruvate dehydrogenase complex (PDHc) deficiency. Both deduced bottom and top...
Effective workflows are essential components in the digitization of biodiversity specimen collections. To date, no comprehensive, community-vetted have been published for digitizing flat sheets and packets plants, algae, fungi, even though latest estimates suggest that only 33% herbarium specimens digitally transcribed, 54% herbaria use a database, 24% imaging specimens. In 2012, iDigBio, U.S. National Science Foundation's (NSF) coordinating center national resource public, nonfederal...
Background . Severe obstructive sleep apnea (OSA) with chronic intermittent hypoxia (IH) is common in idiopathic pulmonary fibrosis (IPF). Here, we evaluated the impact of IH on bleomycin‐ (BLM‐) induced mice. Methods C57BL/6J mice received intratracheal BLM or saline and were exposed to (40 cycles/hour; FiO 2 nadir: 6%; 8 hours/day) air (IA). In four experimental groups, (i) survival; (ii) alveolar inflammation, edema, lung oxidative stress, antioxidant enzymes; (iii) cell apoptosis; (iv)...
We developed an experimental approach with genetically engineered and encapsulated mouse NIH 3T3 fibroblasts to delay the progressive degeneration of photoreceptor cells in dark-eyed Royal College Surgeons rats. These xenogeneic can survive 1.5-mm-long microcapsules made biocompatible polymer AN69 for at least 90 days under vitro vivo conditions because their stable transfection gene 18-kDa form human basic fibroblast growth factor (hFGF-2). Furthermore, when transferred surgically into...
It is important to understand the development of normal retinal vascular system, because it may provide clues for understanding mechanisms underlying neovascularization associated with several retinopathies infancy and adulthood. However, little known about human ocular vascularization. VEGF a key growth factor during one its receptors, KDR, plays pivotal role in endothelial cell proliferation differentiation. The purpose this study was analyze KDR gene expression patterns eye embryonic...
We tested the effect of acetazolamide on blood mechanical properties and pulmonary vascular resistance (PVR) during chronic hypoxia. Six groups rats were either treated or not with (curative: after 10 days hypoxic exposure; preventive: before exposure 40 mg·kg −1 ·day ) exposed to 3 weeks hypoxia (at altitude >5,500 m). They then used assess role artery pressure, cardiac output, volume, haematological haemorheological parameters. Chronic increased haematocrit, viscosity PVR, decreased...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and fatal interstitial lung disease of unknown origin. Alveolar epithelial cells (AECs) play an important role in the fibrotic process as they undergo sustained endoplasmic reticulum (ER) stress, may acquire mesenchymal phenotype through epithelial-to-mesenchymal transition (EMT), two phenomena that could be induced by localized alveolar hypoxia. Here we investigated potential links between hypoxia, ER stress EMT AECs. markers...
<b>Purpose:</b> The mutations responsible for Best vitelliform macular dystrophy (BVMD) are found in a gene called <i>VMD2</i>. <i>VMD2</i> encodes transmembrane protein named bestrophin-1 (hBest1) which is Ca<sup>2+</sup>-sensitive chloride channel. This study was performed to identify disease-specific 27 patients with BVMD. Because this disease characterised by an alteration Cl<sup>−</sup> channel function, patch clamp analysis used test the hypothesis that one of mutated variants causes...
Administration of bone marrow-derived human mesenchymal stem cells (hMSC) reduces lung inflammation, fibrosis, and mortality in animal models injury, by a mechanism not completely understood. We investigated whether hMSC would prevent epithelial-mesenchymal transition (EMT) induced hypoxia primary rat alveolar epithelial cell (AEC). In AEC cultured on semipermeable filters, prolonged hypoxic exposure (1.5% O 2 for up to 12 days) phenotypic changes consistent with EMT, i.e., change...
Abstract Obstructive sleep apnoea ( OSA ) leading to chronic intermittent hypoxia CIH is a common comorbidity associated with idiopathic pulmonary fibrosis IPF ), progressive fatal disease characterized by adverse lung remodeling and parenchymal stiffening. In the mouse model of induced intra‐tracheal instillation ITI bleomycin, we have previously shown that exacerbates fibrosis, similar pre‐exposure . It has been suggested correction positive airway pressure may beneficial effect on...
We analyzed the distribution of FMR1, FXR1, FXR2 mRNA, and FMRP in whole normal human embryos brains fragile X fetuses. The distributions mRNA for 3 genes male female carrier fetuses were similar, with large amounts nervous system several non-nervous tissues. No FMR1 (mRNA protein) was detected no evident neuropathologic abnormalities found fetuses, suggesting that product (FMRP) may have crucial function early stages development. FXR1 had same similar intensity pathologic (female carriers)....
Anemia and hypoxia in rats result an increase factors potentially involved cerebral angiogenesis. Therefore, the aim of this study was to assess effect chronic anemia and/or on cellular responses angiogenesis wild-type anemic transgenic mice. These studies were done erythropoietin-deficient mice (Epo-TAg(h)) normoxia following acute (one day) (14 days, barometric pressure = 420 mmHg) hypoxia. In normoxia, Epo-TAg(h) showed transcript protein levels hypoxia-inducible factor 1alpha...
We assessed ventilatory patterns and responses to hypoxia (HVR) in high-altitude (HA) plateau pikas, repetitively exposed hypoxic burrows, control rats. evaluated the role of neuronal nitric oxide synthase (nNOS) dopamine by using S-methyl-l-thiocitrulline (SMTC) inhibitor haloperidol antagonist, respectively. Ventilation (V̇i) was measured a whole body plethysmograph conscious pikas ( n = 9) low-altitude (LA) rats 7) at different Pi O 2 (56, 80, 111, 150, 186 mmHg) HA acclimatized 9, 8 days...
Abstract Objectives: The aim of this study was to evaluate whether hypoxia and/or erythropoietin would be able modulate proliferation/differentiation processes rat and human myoblasts. Materials methods: Rat L6 primary myoblasts were grown in 21% or 1% O 2 the presence absence recombinant (RhEpo). Presence receptors (EpoR) assayed using RT‐PCR Western blotting techniques. Cell proliferation evaluated by determining doubling time kinetics cultures counting cells. differentiation analysed...
ABSTRACT We report five novel VMD2 mutations in Best's macular dystrophy patients (S16F, I73N, R92H, V235L, and N296S). An SSCP analysis of the 11 exons revealed electrophoretic mobility shifts exclusively 2, 3, 4, 6 8. Direct sequencing indicated that these are caused by mono-allelic transition 6, 8 transversion 3 6. Five "silent" polymorphisms also reported: 213T>C, 323C>A, 1514A>G, 1661C>T, 1712T>C. Hum Mutat 17:235, 2001. © 2001 Wiley-Liss, Inc.
The aim of this study was to assess maximal heart rate (HR) and morphological changes in high altitude living "plateau pikas" rats bred at 2260 m. Rats pikas were catheterized measure HR (2260 m). After baseline measurements, 1 mg/kg atropine (AT) increasing doses isoproterenol (IsoP) (0.1, 1, 10, 100 μg kg) injected into animals. Right (RV) left ventricles (LV) removed calculate Fulton's ratio (LV + septum (S) RV weights) mRNA expression level β1- β2-adrenoceptors, muscarinic m1 m2...
This work aims to study the regulation of glutathione peroxidase and catalase activities in myoblasts from L6 line exposed 21%, 5% 1% O2 during cell differentiation.Rat were grown 1%, or 21% presence absence N-acetyl cysteine. The proliferation was evaluated by determining doubling time kinetics cultures counting cells. differentiation analyzed myogenic fusion index using antibodies against myosin heavy chain. assayed. p110-PI3K/Thr308-Akt pathway studied western blotting. oxidative status...