A5007747198- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Stress Responses and Cortisol
- Lymphatic System and Diseases
- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Spaceflight effects on biology
- RNA Interference and Gene Delivery
- Receptor Mechanisms and Signaling
- Neonatal Respiratory Health Research
Wuhan National Laboratory for Optoelectronics
2021-2024
Huazhong University of Science and Technology
2021-2024
The hybrid chain reaction (HCR), an isothermal and enzyme-free amplification strategy, has found extensive use in fluorescent
Rationale: Hepatic lymphatics are essential for liver homeostasis and immune function. However, the 3D structure spatial distribution of hepatic lymphatic vessels (LVs) need to be confirmed. Moreover, molecular information endothelial cells (LyECs) needs further studied. The bottleneck is lack specific markers or labeling methods Methods: Here, we proposed a method spatiotemporal sequential injection antibodies (STSI-Ab) selectively label LyECs in vivo. In addition, also developed an...
Optical visualization of complex microstructures in the entire organ is essential for biomedical research. However, existing methods fail to accurately acquire detailed whole organs with good morphological and biochemical preservation. This study proposes a cryo-fluorescence micro-optical sectioning tomography (cryo-fMOST) image three dimensions (3D) submicron resolution. The system comprises line-illumination microscope module, cryo-microtome, three-stage refrigeration heat insulation...
Abstract Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts fixed cells and tissues. Despite the recent advances FISH detection methods, it remains challenging achieve high-level imaging with simple workflow. Here, we introduce method prepare long single-strand DNA concatemers (lssDNAc) through controllable rolling-circle amplification (CRCA). Prepared lssDNAcs are used develop AmpFISH workflows. In addition, present its applications different scenarios....
Abstract The forebrain regions displays distinct yet under-characterized gene expression patterns. This study analyzed region-specific feature genes in and Isocortex sub-regions at both transcriptomic proteomic levels. key finding is the observation of low correlation but high functional similarity between mRNA protein expression, which offers insights into connection forms neuronal pathways activity state. Cholinergic neurons (CNs), play a vital role for forebrain’s sensory motor...
Abstract Fluorescence in situ hybridization (FISH) is a powerful tool to visualize transcripts fixed cells and tissues. Despite the recent advances FISH detection methods, it remains challenging achieve high-level imaging with simple workflow. Here, we introduce method prepare long single-strand DNA concatemers (lssDNAc) through controllable rolling-circle amplification (CRCA). Prepared lssDNAcs were used develop novel AmpFISH workflows. In addition, present its applications different...