A5066020693- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Single-cell and spatial transcriptomics
- Advanced Sensor and Energy Harvesting Materials
- Modular Robots and Swarm Intelligence
- Cell Image Analysis Techniques
- Advanced Materials and Mechanics
- Photoacoustic and Ultrasonic Imaging
- Functional Brain Connectivity Studies
- Diatoms and Algae Research
- Cellular Mechanics and Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Identification and Quantification in Food
- Molecular Biology Techniques and Applications
- 3D Printing in Biomedical Research
- Advanced Chemical Sensor Technologies
- Spectroscopy and Chemometric Analyses
- Microfluidic and Bio-sensing Technologies
- Memory and Neural Mechanisms
- Water Quality Monitoring and Analysis
- Wastewater Treatment and Nitrogen Removal
- Advanced Electron Microscopy Techniques and Applications
University of Wollongong
2024
Huazhong University of Science and Technology
2013-2022
Wuhan National Laboratory for Optoelectronics
2017-2022
The dorsal raphe nucleus (DR) and median (MR) contain populations of glutamatergic GABAergic neurons that regulate diverse behavioral functions. However, their whole-brain input-output circuits remain incompletely elucidated. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive atlas inputs outputs in the DR MR. found these received from similar upstream brain regions. same had divergent projection patterns differences critical...
A neural circuit is a structural-functional unit of achieving particular information transmission and processing, have various inputs, outputs molecular phenotypes. Systematic acquisition comparative analysis the features circuits are crucial to elucidating operating mechanisms brain function. However, no efficient, systematic approach available for describing phenotypes specific at whole scale. In this study, we developed rapid whole-brain optical tomography method devised an efficient map...
To decipher the organizational logic of complex brain circuits, it is important to chart long-distance pathways while preserving micron-level accuracy local network. However, mapping neuronal projections with individual-axon resolution in large and primate still challenging. Herein, we describe a highly efficient pipeline for three-dimensional entire macaque subcellular resolution. The includes novel poly-N-acryloyl glycinamide (PNAGA)-based embedding method long-term structure fluorescence...
The glutamatergic and GABAergic neurons in the ventral tegmental area (VTA) substantia nigra pars compacta (SNc) mediated diverse brain functions. However, their whole-brain neural connectivity has not been comprehensively mapped. Here we used virus tracers to characterize inputs outputs of VTA SNc. We found that these received similar from upstream regions, but some quantitative differences were also observed. Neocortex dorsal striatum provided a greater share input neurons. Periaqueductal...
Optical visualization of complex microstructures in the entire organ is essential for biomedical research. However, existing methods fail to accurately acquire detailed whole organs with good morphological and biochemical preservation. This study proposes a cryo-fluorescence micro-optical sectioning tomography (cryo-fMOST) image three dimensions (3D) submicron resolution. The system comprises line-illumination microscope module, cryo-microtome, three-stage refrigeration heat insulation...
Cutting tissues into ultrathin slices is highly desired in sectioning-based organ-wide imaging. However, it difficult to perform tissue cutting at a high speed with excellent quality. Here, we design precision vibratome based on paired double parallelogram flexure, which enables vibrating blade move strictly along straight line. Meanwhile, develop high-speed method that does not compromise quality, the operated frequency mode. The characterized parasitic motion errors of 180-Hz were less...
Leveraging the unique attributes of functional soft materials to generate force and deformation, significant advancements in actuators are driving evolution smart robotics. Liquid metals (LMs), known for their high deformability tunable morphology, demonstrate remarkable actuating capabilities through controllable surface tension. Inspired by predation method chameleons, this work introduces a bioinspired LM actuator (BLMA) modulating morphology LM. This BLMA enables high‐strain (up 170%)...
Resin embedding is widely used to dissect the fine structure of bio-tissue with electron and optical microscopy. However, it difficult embed large-volume tissues resin. Here, we modified formula LR-White resin prevent sample cracking during polymerization process applied this method intact brains mouse, ferret, macaque. Meanwhile, increased fluorescence preservation rate for green fluorescent protein (GFP) from 73 ± 4.0% 126 3.0% tdTomato 60 3.3% 117 2.8%. Combined whole-brain imaging...
Acquiring brain-wide composite information of neuroanatomical and molecular phenotyping is crucial to understand brain functions. However, current whole-brain imaging methods based on mechnical sectioning haven't achieved acquisition both due the lack appropriate immunostaining embedded samples. Here, we present a novel strategy acquiring structural maps in same brain, combining subsequent automated-collected slices. We developed system capable automatically then collecting imaged tissue...
Abstract The dorsal raphe nucleus (DR) and median (MR) contain populations of glutamatergic GABAergic neurons regulating diverse behavioral functions. Their whole-brain input-output circuits remain incompletely understood. We used viral tracing combined with fluorescence micro-optical sectioning tomography to generate a comprehensive atlas inputs outputs in the DR MR. discovered that these receive from similar upstream brain regions. same have divergent projection patterns differences...