- Microbial Metabolic Engineering and Bioproduction
- Microbial bioremediation and biosurfactants
- Biofuel production and bioconversion
- Methane Hydrates and Related Phenomena
- Microbial Community Ecology and Physiology
- Building Energy and Comfort Optimization
- Enzyme Catalysis and Immobilization
- Viral Infectious Diseases and Gene Expression in Insects
- Microstructure and mechanical properties
- Algal biology and biofuel production
- Geological Studies and Exploration
- Fermentation and Sensory Analysis
- Metabolomics and Mass Spectrometry Studies
- Electromagnetic Effects on Materials
- Mass Spectrometry Techniques and Applications
- Infrared Thermography in Medicine
- Bacterial Genetics and Biotechnology
- Material Properties and Applications
- Hydrogen embrittlement and corrosion behaviors in metals
- Microstructure and Mechanical Properties of Steels
- Subcritical and Supercritical Water Processes
- Metal Alloys Wear and Properties
- Enzyme Production and Characterization
- Advanced ceramic materials synthesis
- Ecology and Conservation Studies
Kitami Institute of Technology
2016-2025
Kwansei Gakuin University
2021
National Institute of Advanced Industrial Science and Technology
2006-2018
Japan Agency for Marine-Earth Science and Technology
2010-2016
Kobe University
2007-2008
Tokyo University of Science
2007
Osaka University
2005-2006
Hiroshima Institute of Technology
1987-2004
Asahi Glass (Belgium)
1999-2002
Asahi Glass (Japan)
2000-2002
Sophorolipids (SLs) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts, and have been widely developed for various applications. In this study, we searched novel SLs, aiming to broaden the functions application range. As a result of screening based on phylogenetic information known SL producer, found that Candida batistae CBS 8550 produces new types SLs. Interestingly, present product mainly constituted acid-form SLs (more than 60% total SLs), considerably...
Pseudozyma antarctica is one of the best producers glycolipid biosurfactants known as mannosylerythritol lipids (MELs), which show not only excellent surface-active properties but also versatile biochemical actions. In order to obtain a variety producers, all species genus were examined for their production MELs from soybean oil. fusiformata, P. parantarctica and tsukubaensis newly identified be MEL producers. Of strains tested, gave yield (30 g L(-1)). The obtained corresponded those...
The aqueous-phase behavior of mannosylerythritol lipid A (MEL-A), which is a glycolipid biosurfactant produced from vegetable oils by yeast strains the genus Pseudozyma, was investigated using polarized optical microscopy, small-angle X-ray scattering (SAXS), and differential scanning calorimetry (DSC). MEL-A found to self-assemble into variety distinctive lyotropic liquid crystals including sponge (L3), bicontinuous cubic (V2), lamella (Lα) phases. On basis SAXS measurements, we determined...
The use of biodegradable plastics can reduce the accumulation environmentally persistent plastic wastes. rate degradation depends on environmental conditions and is highly variable. Techniques for achieving more consistent are needed. However, only a few microorganisms involved in process have been isolated so far from environment. Here, we show that Pseudozyma spp. yeasts, which common phyllosphere easily plant surfaces, displayed strong activity films made poly-butylene succinate or...
Mannosylerythritol lipids (MELs) are glycolipid biosurfactants produced by the yeast strains of genus Pseudozyma. These show not only excellent surface-active properties but also versatile biochemical actions. In course MEL production from soybean oil P. shanxiensis, new extracellular glycolipids (more hydrophilic than previously reported MELs) were found in culture medium. As a result structural characterization, identified as mixture 4-O-[(2′,...
A basidiomycetous yeast, Pseudozyma graminicola CBS 10092, was found to accumulate a large amount of glycolipids in the cultured medium when grown on soybean oil as sole carbon source. Based thin layer chromatography, extracellular gave spots corresponding those mannosylerythritol lipids (MELs), which are highly functional and promising biosurfactants. From structural characterization by 1H 13C NMR, main product identified 4-O-[(4′-mono-O-acetyl-2′,...
The basidiomycetous yeast-like fungus Pseudozyma hubeiensis strain SY62 is capable of producing an abundant amount the glycolipid biosurfactant mannosylerythritol lipids (MELs), which are a major component monoacetylated MEL (MEL-C). To reveal synthetic pathway MELs SY62, we present 18.44-Mb draft genome sequence.
We discovered that Candida floricola ZM1502 is capable of selectively producing the promising hydrophilic biosurfactants, acid-form sophorolipids (SLs), from glycerol. However, productivity was very low (approximately 3.5 g L-1) under initial culture conditions. Here, we describe design medium for abundant production SLs by C. using waste glycerol and hydrophobic substrates in order to develop a method SL disposal produced oleo-chemical industries. Urea provided best nitrogen source among...
Mannosylerythritol lipids (MELs) are one of the most promising biosurfactants known, because their multifunctionality and biocompatibility. In order to attain an efficient production MELs, Pseudozyma parantarctica JCM 11752T, which is a newly identified strain genus, was examined for productivity MELs at different culture conditions. The yeast showed significant cell growth di-acylated even 36°C. contrast, on conventional high-level MEL producers including P. rugulosa, yield considerably...