A5034831021- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Membrane Separation and Gas Transport
- Enzyme Catalysis and Immobilization
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Microbial metabolism and enzyme function
- biodegradable polymer synthesis and properties
- Catalysis for Biomass Conversion
- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Synthesis and properties of polymers
- Fungal and yeast genetics research
- Microbial Community Ecology and Physiology
- Microplastics and Plastic Pollution
- Fermentation and Sensory Analysis
- Supramolecular Self-Assembly in Materials
- Mass Spectrometry Techniques and Applications
- Mesoporous Materials and Catalysis
- Virus-based gene therapy research
- Membrane Separation Technologies
- Polyamine Metabolism and Applications
- Pickering emulsions and particle stabilization
- Zeolite Catalysis and Synthesis
National Institute of Advanced Industrial Science and Technology
2012-2021
University of the Ryukyus
2009
Kagoshima University
2009
Tokyo University of Science
2007
Sustainable Innovation (Sweden)
2005
Panasonic (Japan)
2002
National Institute for Materials Science
1993-2001
Institut de Chimie
2001
Food Research Institute
1998
University of Tsukuba
1990-1997
Sophorolipids (SLs) are glycolipid biosurfactants abundantly produced from different feedstocks by yeasts, and have been widely developed for various applications. In this study, we searched novel SLs, aiming to broaden the functions application range. As a result of screening based on phylogenetic information known SL producer, found that Candida batistae CBS 8550 produces new types SLs. Interestingly, present product mainly constituted acid-form SLs (more than 60% total SLs), considerably...
Mannosylerythritol lipids (MELs) are biosurfactants known for their versatile interfacial and biochemical properties. To broaden application in cosmetics, we investigated the antioxidant properties of different MEL derivatives (MEL-A, -B, -C) by using a 1,1-diphenyl-2-picryl hydrazine (DPPH) free-radical- superoxide anion-scavenging assay. All tested showed activity vitro, but at lower levels than those arbutin. Of MELs, MEL-C, which is produced from soybean oil Pseudozyma hubeiensis,...
The biological activities of 7 microbial extracellular glycolipids including mannosylerythritol lipid (MEL)-A, MEL-B, polyol (PL), rhamnolipid (RL), sophorose (SL), succinoyl trehalose (STL)-1, and STL-3 were investigated. All except for RL found to induce cell differentiation instead proliferation in the human promyelocytic leukemia line HL60. To identify direction induced cells, leukocyte esterase cytologically investigated, results showed that MEL-A, PL HL60 differentiate into...
Pseudozyma antarctica is one of the best producers glycolipid biosurfactants known as mannosylerythritol lipids (MELs), which show not only excellent surface-active properties but also versatile biochemical actions. In order to obtain a variety producers, all species genus were examined for their production MELs from soybean oil. fusiformata, P. parantarctica and tsukubaensis newly identified be MEL producers. Of strains tested, gave yield (30 g L(-1)). The obtained corresponded those...
Coacervate (L3 phase) formation of the single component "natural" glycoliped biosurfactant, MEL-A, was observed for first time by using an optical microscope, a confocal laser scanning microscope (CLSM), and freeze-fracture electron (FF-TEM). It also found that only slight decrease in spontaneous curvature resulting from absence one acetyl group on headgroup induced drastic morphological change 3D self-assembled structure coacervates to ordered vesicles (Lalpha phase).
A yeast strain, isolated from the exudate of a tree, accumulated biosurfactants abundantly when grown on soybean oil as sole carbon source. The were found to be mixture 4 mannosylerythritol lipids, including two new lipids major components. components, which amounted about 80% total determined 4-O-(di-O-acetyl-di-O-alkanoyl-β-D-mannopyranosyl)-erythritol and 4-O-(mono-O-acetyl-di-O-alkanoyl-β-D-mannopyranosyl)-erythritol. strain was identified Candida antarctica (Goto et al.) Kurtzman al.
Journal Article Extracellular Accumulation of Mannosylerythritol Lipids by a Strain Candida antarctica Get access Dai Kitamoto, Kitamoto Institute Applied Biochemistry, University Tsukuba, Ibaraki 305, Japan Present address: National Chemical Laboratory for Industry, Japan. Search other works this author on: Oxford Academic Google Scholar Shyunichi Akiba, Akiba Kashima Research Laboratories Kao Corporation, Kamisu-cho, 314-02, Chieko Hioki, Hioki Takeshi Tabuchi To whom correspondence should...
ABSTRACT Glyceric acid (GA), an unfamiliar biotechnological product, is currently produced as a small by-product of dihydroxyacetone production from glycerol by Gluconobacter oxydans . We developed method for the efficient GA target compound new surplus applications in biodiesel and oleochemical industries. investigated ability 162 acetic bacterial strains to produce found that patterns productivity enantiomeric compositions obtained several differed significantly. The growth parameters two...
The aqueous-phase behavior of mannosylerythritol lipid A (MEL-A), which is a glycolipid biosurfactant produced from vegetable oils by yeast strains the genus Pseudozyma, was investigated using polarized optical microscopy, small-angle X-ray scattering (SAXS), and differential scanning calorimetry (DSC). MEL-A found to self-assemble into variety distinctive lyotropic liquid crystals including sponge (L3), bicontinuous cubic (V2), lamella (Lα) phases. On basis SAXS measurements, we determined...
ABSTRACT The basidiomycetous yeast Pseudozyma antarctica T-34 is an excellent producer of mannosylerythritol lipids (MELs), members the multifunctional extracellular glycolipids, from various feedstocks. Here, genome sequence P. was determined and annotated. Analysis might provide insights into properties this that make it superior for use in production functional leading to further development industrial applications.
Self-assembling properties of "natural" glycolipid biosurfactants, mannosyl-erythritol lipids A and B (MEL-A, MEL-B), which are abundantly produced from yeast strains, were investigated by using the fluorescence-probe method, dynamic light-scattering (DLS) analysis, freeze-fracture transmission electron microscopy (FF-TEM), synchrotron small/wide-angle X-ray scattering (SAXS/WAXS) among other methods. Both MEL-A MEL-B exhibit excellent self-assembly at extremely low concentrations; they...
Abstract Microbial extracellular glycolipids, succinoyl trehalose lipid (STL), and mannosylerythritol (MEL) inhibited the growth of a human promyelocytic leukemia cell line, HL60, induced their morphological changes. The results specific nonspecific leukocyte esterase activities showed that STL monocytotic differentiation while MEL granulocytic differentiation. markedly increased common differentiation‐associated characteristics in monocytes granulocytes, such as nitroblue tetrazolium (NBT)...
In order to evaluate the feasibility of using lecithins for nanocapsules including functional food materials, liposomes were prepared from different commercially available (SLP-WHITE, SLP-PC70 and PL30S) by Bangham method, their physicochemical properties examined a confocal laser scanning microscopy (CLSM) measurements trapping efficiency. There was little difference in efficiency among three types liposomes. all cases, clearly increased with an increase lecithin concentration up 10 wt % ,...