A5089809397- Phytochemicals and Antioxidant Activities
- Free Radicals and Antioxidants
- Alzheimer's disease research and treatments
- Antioxidant Activity and Oxidative Stress
- Phytochemistry and Biological Activities
- Tea Polyphenols and Effects
- Essential Oils and Antimicrobial Activity
- Computational Drug Discovery Methods
- Advanced Glycation End Products research
- Atherosclerosis and Cardiovascular Diseases
- Ginkgo biloba and Cashew Applications
- Heavy Metals in Plants
- Tryptophan and brain disorders
- Insect and Pesticide Research
- Sirtuins and Resveratrol in Medicine
- Synthesis and biological activity
- Neurological diseases and metabolism
- Analytical Chemistry and Chromatography
- Biochemical effects in animals
- Prion Diseases and Protein Misfolding
- Nigella sativa pharmacological applications
- Food Quality and Safety Studies
- Bee Products Chemical Analysis
- Natural Antidiabetic Agents Studies
- Pharmacological Effects of Natural Compounds
Comenius University Bratislava
2012-2025
Institute of Botany
2013
Detská Fakultná Nemocnica s Poliklinikou
2013
The well-known antioxidant activity of red wine is explained mostly by its polyphenols content, where the final effect based on components’ interaction. aim our work was study interaction three polyphenols—quercetin, resveratrol and caffeic acid—alone in their equimolar binary ternary mixtures different antioxidant/scavenging assays (inhibition 2-deoxy-D-ribose degradation hydroxyl radical, FRAP, Fe(III) reducing power, DPPH, ABTS NO scavenging, respectively). Interaction analysis, median...
Abstract The amyloidoses are diseases associated with nonnative folding of proteins and characterized by the presence protein amyloid aggregates. ability quercetin, resveratrol, caffeic acid, their equimolar mixtures to affect aggregation hen egg white lysozyme in vitro was detected Thioflavin T fluorescence assay. anti‐amyloid activities tested polyphenols were evaluated median depolymerization concentrations DC 50 inhibition IC . Single substances more efficient (by at least one order)...
Abstract Exogenous insulin, used as a therapeutic agent for diabetes, forms insoluble deposits containing amyloid fibrillar structures near the administration site. We have analyzed in vitro anti-amyloid activity of four green tea constituents: (-)-epigallocatechin gallate (EGCG), (-)-epicatechin (EC), gallic acid (GA), caffeine (CF), and their equimolar mixtures. Regarding individually tested compounds, only EGCG inhibited fibrillization process. The individual EC, GA, CF molecules were...
Caffeic acid (CA) is one of the most abundant natural compounds present in plants and has a broad spectrum beneficial pharmacological activities. However, some cases, synthetic derivation original molecules can expand their scope. This study focuses on synthesis caffeic phosphanium derivatives with ambition increasing biological Four salts (CAPs) were synthesized tested for cytotoxic, antibacterial, antifungal, amoebicidal activity vitro, aim identifying best area medicinal use. CAPs...
Vascular smooth muscle cell (VSMC) proliferation contributes to the development of atherosclerosis. Red wine consumption due polyphenol content has been reported counteract atherosclerosis progression possibly through inhibition VSMC proliferation, among other mechanisms. In this study we investigate antiproliferative activity four polyphenols: resveratrol, quercetin, ethyl gallate, and (+)-catechin in rat aortic VSMC. All polyphenols inhibited serum-induced when applied as a single...
Amyloidoses represent a group of pathological conditions characterized by amyloid fibrils accumulating in the form deposits intra- or extracellular space, leading to tissue damage. The lysozyme from hen egg-white (HEWL) is often used as universal model protein study anti-amyloid effects small molecules. vitro activity and mutual interactions green tea leaf constituents: (-)-epigallocatechin gallate (EGCG), (-)-epicatechin (EC), gallic acid (GA), caffeine (CF) their equimolar mixtures were...
Abstract Aim Enterococcus faecalis is a significant pathogen in endodontic infections, often leading to treatment failure root canal therapy and contributing chronic infections. The growing concern over antimicrobial resistance highlights the need for alternative approaches. This study aims evaluate antibacterial properties of aqueous green tea ( Camellia sinensis ) peppermint Mentha × piperita extracts, focusing on their phytochemical profiles activity against E. , major Material Methods...
Unlike its aerial parts, the underground parts of Mentha have so far been studied only marginally. By examining polyphenolic fingerprint, antioxidant efficacy and mutual behaviour mixtures mint rhizomes, our study presents a modest contribution to addressing this gap. Firstly, we examined composition rhizomes: × piperita cv. ‘Perpeta’ (MPP), M. longifolia (ML), villosa ‘Snežna’ (MVS). Our LC-MS-DAD analysis revealed presence ten compounds belonging groups phenolic acids flavonoids, which...
The compositions of leaf infusions three genotypes Lycopus europaeus L. with origins in central Europe, namely A (LeuA), B (LeuB), and C (LeuC), one genotype exaltatus (Lex), were examined by LC-MS-DAD (Liquid Chromatography Mass Spectrometry Diode Array Detection) analysis. This revealed the presence thirteen compounds belonging to groups phenolic acids flavonoids, a predominance rosmarinic acid (RA) luteolin-7-O-glucuronide (LGlr). antimicrobial activity was tested on collection strains...
Beneficial effects of red wine polyphenols on cardiovascular health are well known. The aim our research was an interaction study four – resveratrol (R), quercetin (Q), kaempferol (KF) and isorhamnetin (IR) their ability to activate endothelial NO synthase (eNOS) promoter when used alone in equimolar mixtures. To determine activity, we performed a luciferase reporter gene assay EA.hy926 cells stably transfected with construct containing eNOS promoter. Bradford also account the cytotoxicity...
Abstract The study of drug synergy plays a prominent role in the search for combinations with beneficial interactions. Firstly, this process, drug‐effect response individual parts and mixture needs to be derived. This function is usually well described by Hill (or other logistic or sigmoid) curve. Due its boundedness, it allows measured data normalized. normalized can then processed interaction analysis using Loewe, Bliss, models evaluate possible antagonism two more drugs. However,...
Abstract Resveratrol and caffeic acid belong to plant polyphenols are known for their antioxidant effects. The aim of our research was study impact on Maillard reaction. This one occurs when the reducing saccharides react with amino groups biomolecules including proteins, alter protein conformation transform variety advanced glycation end products (AGEs). AGEs exhibit browning generate fluorescence. There exist expectations that this oxidative glycosylation could be prevented by...