A5002573915- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Computational Drug Discovery Methods
- Cholinesterase and Neurodegenerative Diseases
- Proteins in Food Systems
- Protein Interaction Studies and Fluorescence Analysis
- Multiple Myeloma Research and Treatments
- Enzyme Structure and Function
- Supramolecular Self-Assembly in Materials
- Nanoparticle-Based Drug Delivery
- Prion Diseases and Protein Misfolding
- Drug Transport and Resistance Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Trace Elements in Health
- Cancer Treatment and Pharmacology
- Toxin Mechanisms and Immunotoxins
- Neuroscience and Neuropharmacology Research
- Curcumin's Biomedical Applications
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Advanced Drug Delivery Systems
- Click Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Advanced Glycation End Products research
- Dementia and Cognitive Impairment Research
Slovak Academy of Sciences
2016-2025
Institute of Experimental Physics of the Slovak Academy of Sciences
2016-2025
Biomedical Research Center of the Slovak Academy of Sciences
2025
University of Pavol Jozef Šafárik
2014-2020
Technical University of Košice
2015-2018
Ho Chi Minh City University of Science
2017
Sapienza University of Rome
2007
Max Planck Society
2006
Max Planck Unit for Structural Molecular Biology
2004-2005
The abnormal aggregation of tau protein into paired helical filaments (PHFs) is one the hallmarks Alzheimer's disease. Aggregation takes place in cytoplasm and could therefore be cytotoxic for neurons. To find inhibitors PHF we screened a library 200,000 compounds. hits found inhibition assay were also tested their ability to dissolve preformed PHFs. results obtained using thioflavin S fluorescence detection quantification solution, tryptophan tryptophan-containing mutants tau, confirmed by...
Peptide amyloid aggregation is a hallmark of several human pathologies termed diseases. We have investigated the effect electrostatically stabilized magnetic nanoparticles Fe3O4 on lysozyme, as prototypical amyloidogenic protein. Thioflavin T fluorescence assay and atomic force microscopy were used for monitoring inhibiting disassembly activity Fe3O4. found that are able to interact with lysozyme amyloids in vitro leading reduction aggregates, thus promoting depolymerization; studied also...
A structural series of 7-MEOTA-adamantylamine thioureas was designed, synthesized and evaluated as inhibitors human acetylcholinesterase (hAChE) butyrylcholinesterase (hBChE). The compounds were prepared based on the multi-target-directed ligand strategy with different linker lengths (n = 2–8) joining well-known NMDA antagonist adamantine hAChE inhibitor 7-methoxytacrine (7-MEOTA). Based in silico studies, these proved dual binding site character capable simultaneous interaction peripheral...
The review summarizes research into the highly relevant topics of cholinesterase and amyloid aggregation inhibitors connected to tacrine congeners, both which are associated with neurogenerative diseases. Various opinions will be discussed regarding dual binding site characterized by increased inhibitor potency against acetylcholin/butyrylcholine esterase formation. It is suggested that these compounds can raise levels acetylcholine active site, also prevent aggregation. In connection this...
Abstract The amyloidoses are diseases associated with nonnative folding of proteins and characterized by the presence protein amyloid aggregates. ability quercetin, resveratrol, caffeic acid, their equimolar mixtures to affect aggregation hen egg white lysozyme in vitro was detected Thioflavin T fluorescence assay. anti‐amyloid activities tested polyphenols were evaluated median depolymerization concentrations DC 50 inhibition IC . Single substances more efficient (by at least one order)...
We studied the effect of water-soluble fullerenol C<sub>60</sub>(OH)<sub>16</sub> on amyloid fibrillization Aβ<sub>40</sub> peptide <italic>in vitro</italic> and silico</italic>.
Abstract Exogenous insulin, used as a therapeutic agent for diabetes, forms insoluble deposits containing amyloid fibrillar structures near the administration site. We have analyzed in vitro anti-amyloid activity of four green tea constituents: (-)-epigallocatechin gallate (EGCG), (-)-epicatechin (EC), gallic acid (GA), caffeine (CF), and their equimolar mixtures. Regarding individually tested compounds, only EGCG inhibited fibrillization process. The individual EC, GA, CF molecules were...
The histopathological diagnosis of Alzheimer's disease relies on two kinds proteinaceous aggregates: the extracellular plaques built from filaments Abeta-peptide and intracellular tangles consisting tau polymerized into Paired Helical Filaments (PHFs). order aggregation events is still under debate, but it well accepted that tau-related changes have an important impact viability neurons. In neurons, early morphological are seen in axons which begin to loose retract synapses. This process...
Degenerative diseases, such as Alzheimer's and prion well type II diabetes, have a pathogenesis associated with protein misfolding, which routes amyloid formation. Recent strategies for designing small-molecule polypeptide antiamyloid inhibitors are mainly based on mature fibril structures containing cross β-sheet structures. In the present study, we tackled hypothesis that rational design of agents can target native proteins might offer advantageous prospect to effective therapeutics....
Abstract Nanoparticles have great potential to be used in various biomedical applications, including therapy or diagnosis of amyloid‐related diseases. The physical and chemical properties iron oxide superparamagnetic nanoparticles (MNPs) functionalized with different amino acids (AAs), namely, lysine (Lys), glycine (Gly), tryptophan (Trp), been characterized. cytotoxicity their effect on amyloid fibrillization lysozymes vitro was also verified. AA‐MNPs under study are nontoxic human SHSY5Y...
Pathogenesis of amyloid-related diseases is associated with the presence protein amyloid deposits. Insulin amyloids have been reported in a patient diabetes undergoing treatment by injection insulin and causes problems production storage this drug application pumps. We studied interference fibrils series 18 albumin magnetic fluids (MFBSAs) consisting magnetite nanoparticles modified different amounts bovine serum (w/w BSA/Fe3O4 from 0.005 up to 15). found that MFBSAs are able destroy vitro....
While amyloid-related diseases are at the center of intense research efforts, no feasible cure is currently available for these diseases. The experimental and computational techniques were used to study ability glyco-acridines prevent lysozyme amyloid fibrillization in vitro. Fluorescence spectroscopy atomic force microscopy have shown that inhibit aggregation lysozyme; inhibition efficiency characterized by half-maximal concentration IC50 was affected structure derivative. We next...
The most frequent disease of the locomotor system is osteoarthritis (OA), which, as a chronic joint disease, might benefit more from nutrition than acute illnesses. Collagen hydrolysates (CHs) are peptidic mixtures that often used nutraceuticals for OA. Three CHs were characterized biochemically and pharmacologically. Our biophysical (MALDI-TOF-MS, NMR, AFM) fluorescence assays revealed marked differences between fish (Peptan® F 5000, Peptan® 2000) porcine (Mobiforte®) origin with respect to...
Self-assembly of Aβ peptides into amyloid aggregates has been suggested as the major cause Alzheimer’s disease (AD). Nowadays, there is no medication for AD, but experimental data indicate that reversion process aggregation reduces symptoms disease. In this paper, all 8000 tripeptides were studied their ability to destroy fibrils. The docking method and more sophisticated MM-PBSA (molecular mechanics Poisson–Boltzmann surface area) employed calculate binding affinity mode these depolymerize...
Amyloidoses represent a group of pathological conditions characterized by amyloid fibrils accumulating in the form deposits intra- or extracellular space, leading to tissue damage. The lysozyme from hen egg-white (HEWL) is often used as universal model protein study anti-amyloid effects small molecules. vitro activity and mutual interactions green tea leaf constituents: (-)-epigallocatechin gallate (EGCG), (-)-epicatechin (EC), gallic acid (GA), caffeine (CF) their equimolar mixtures were...
Silica-coated magnetic nanoparticles can be utilized for the separation of nucleic acids, which is an important step in many clinical procedures. A series silica-functionalized superparamagnetic iron oxide (SiO2@MNPs) was synthesized to efficiently separate acids sensitive detection viral infections. Magnetite (MNPs) coated with different thicknesses silica layer resulting from ratios tetraethylorthosilicate (TEOS) precursor magnetite (TEOS/MNPs = 0.4, 1.0, 2.0, 3.0, 4.0, 5.0 ml/g) have been...
The sodium/calcium exchanger (NCX) type 1 has been well described in various cancers, but little is known about the other two NCX types (NCX2 and NCX3). In this study, we used selective blocker of NCX3 - YM-244769 to investigate changes apoptosis induction, migration, proliferation, intracellular calcium ATP four cancer cell lines DLD1, HeLa, MDA-MB-231 JIMT1. all observed a concentration-dependent increase number apoptotic cells, as reduced migration proliferation. Induction hypoxic...