A5044264647- Pancreatic function and diabetes
- Circadian rhythm and melatonin
- Dietary Effects on Health
- Birth, Development, and Health
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Regulation of Appetite and Obesity
- Sleep and related disorders
- Diet and metabolism studies
- Diabetes Treatment and Management
- Genetics, Aging, and Longevity in Model Organisms
- Biochemical Analysis and Sensing Techniques
- Heart Rate Variability and Autonomic Control
- Diabetes and associated disorders
- Diabetes Management and Research
- Obesity, Physical Activity, Diet
- Sports Performance and Training
- Sodium Intake and Health
- Education Pedagogy and Practices
- Bariatric Surgery and Outcomes
- Light effects on plants
- Neuropeptides and Animal Physiology
- Cardiovascular and exercise physiology
- Exercise and Physiological Responses
Universidade Estadual de Campinas (UNICAMP)
2001-2025
Universidade Estadual de Maringá
2015-2022
Universidade Católica de Brasília
2016-2021
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2010-2016
Universidade Estadual de Ponta Grossa
2015
Creative Research Enterprises (United States)
2015
Karolinska Institutet
2008-2014
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2013-2014
Universitat de Miguel Hernández d'Elx
2010-2013
Uppsala University
2000-2006
Bisphenol A (BPA) is a widespread endocrine-disrupting chemical used as the base compound in manufacture of polycarbonate plastics. In humans, epidemiological evidence has associated BPA exposure adults with higher risk type 2 diabetes and heart disease.We examined action environmentally relevant doses on glucose metabolism mice during pregnancy impact these females later life. We also investigated consequences utero to metabolic parameters pancreatic function offspring.Pregnant were treated...
Bisphenol-A (BPA) is one of the most widespread endocrine disrupting chemicals (EDC) used as base compound in manufacture polycarbonate plastics. Although evidence points to consider exposure BPA a risk factor for insulin resistance, its actions on whole body metabolism and insulin-sensitive tissues are still unclear. The aim present work was study effects low doses peripheral adult mice. Adult mice were treated with subcutaneous injection 100 µg/kg or vehicle 8 days. Whole energy...
Melatonin has a number of beneficial metabolic actions and reduced levels melatonin may contribute to type 2 diabetes. The present study investigated the pathways involved in effects on mitochondrial function insulin resistance rat skeletal muscle. effect was tested both vitro isolated rats muscle cells vivo using pinealectomized (PNX). Insulin induced by treating primary with palmitic acid for 24 hr. Insulin-stimulated glucose uptake followed decreased phosphorylation AKT which prevented my...
Hypersecretion of glucagon contributes to the dysregulation glucose homeostasis in diabetes. To clarify underlying mechanism, glucose-regulated secretion was studied mouse pancreatic islets and clonal hamster In-R1-G9 glucagon-releasing cells. Apart from well-known inhibition with maximal effect around 7 mmol/l glucose, we discovered that showed paradoxical stimulation release at 25-30 cells 12-20 sugar. Whereas absence inhibited by hyperpolarization diazoxide, this agent tended further...
Disturbances of circadian rhythms have been associated with obesity and type 2 diabetes. The nuclear receptor Rev-erbα was suggested to link metabolism in peripheral tissues. aim the present study dissect role this clock gene pancreatic β-cell function analyze whether its expression is modulated by leptin diet-induced obesity. To address Rev-erbα, we used small interfering RNA mouse islet cells MIN-6 cells. Cell proliferation measured bromodeoxyuridine incorporation, apoptosis terminal...
Insulin resistance and hyperinsulinemia are generally associated with obesity. Obese nondiabetic individuals develop a compensatory β-cell response to adjust insulin levels the increased demand, maintaining euglycemia. Although several studies indicate that this compensation relies on structural changes, existence of functional adaptations is incompletely understood. Here, we fed female mice high-fat diet (HFD) for 12 weeks. These animals became obese, hyperinsulinemic, insulin-resistant,...
Disruption of pancreatic clock genes impairs beta-cell function, leading to the onset diabetes. Despite importance alpha-cells in regulation glucose homeostasis and diabetes pathophysiology, nothing is known about role these cells. Here, we identify gene Rev-erb alpha as a new intracellular regulator glucagon secretion. down-regulation by siRNA (60–70% inhibition) alphaTC1-9 cells inhibited low-glucose induced secretion (p<0.05) led decrease key exocytotic machinery. The agonist GSK4112...
Circadian clocks coordinate physiological, behavioral, and biochemical events with predictable daily environmental changes by a self-sustained transcriptional feedback loop. CLOCK ARNTL are activators that regulate Per Cry gene expression. PER CRY inhibit their own transcription, turnover allows this cycle to restart. The transcription factors BHLHB2 BHLHB3 repress activation, whereas orphan nuclear receptors of the NR1D ROR families control Arntl Here we show AMP-activated protein kinase...
Clock gene expression was associated with different components of metabolic syndrome (MS) in human adipose tissue. However, no study has been done to compare the clock genes visceral tissue (VAT) from lean and obese subjects its clinical implications. Therefore, we studied women endogenous 24 h isolated adipocytes association MS components. VAT obtained (BMI 21–25 kg/m2; n = 21) morbidly >40 28). The pattern analyzed every 6 hours using RT-PCR. Correlation data by Spearman analysis. showed...
Abstract Patients in maintenance hemodialisys (HD) present sleep disorders, increased inflammation, unbalanced redox profiles, and elevated biomarkers representing endothelial dysfunction. Resistance training (RT) has shown to mitigate the loss of muscle mass, strength, improve inflammatory function while decreasing oxidative stress for those HD. However, relation between factors quality are inadequately described. The aim this study was verify effects 3 months RT on quality, balance, nitric...
The mechanism by which protein malnutrition impairs glucose-stimulated insulin secretion in the pancreatic beta-cell is not completely known but may be related to alterations signaling events involved release. Here, we aimed study stimulus-secretion coupling of beta-cells from mice fed with low-protein (LP) diet or normal-protein (NP) for 8 wk after weaning. Patch-clamp measurements isolated cells showed that LP had a resting membrane potential was more hyperpolarized than controls....
AMP-activated protein kinase (AMPK) is a heterotrimeric complex, composed of catalytic subunit (α) and two regulatory subunits (β γ), which act as metabolic sensor to regulate glucose lipid metabolism. A mutation in the γ3 (AMPKγ3(R225Q)) increases basal AMPK phosphorylation, while concomitantly reducing sensitivity AMP. AMPKγ3(R225Q) (γ3(R225Q)) transgenic mice are protected against dietary-induced triglyceride accumulation insulin resistance. We determined whether skeletal muscle-specific...
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of the insulin signalling pathway. It has been demonstrated that PTP1B deletion protects against development obesity and Type 2 Diabetes, mainly through its action on peripheral tissues. However, little attention paid to role in β-cells. Therefore, our aim was study pancreatic Silencing expression β-cell line (MIN6 cells) reveals significance this endoplasmic reticulum bound regulation cell proliferation apoptosis. Furthermore,...
REV-ERB ALPHA has been shown to link metabolism with circadian rhythms. We aimed identify new polymorphisms in the promoter of and tested whether these could be associated obesity Spanish population. Of 1197 subjects included our study, 779 were obese (BMI 34.38±3.1 kg/m2) 418 lean 23.27±1.5 kg/m2). In group, 469 had type 2 diabetes. Genomic DNA from all was obtained peripheral blood cells genotyping analyzed by High Resolution Melting. found six identified rs939347 as a SNP highest...