A5056949894- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Drug Delivery Systems
- Inhalation and Respiratory Drug Delivery
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Peptidase Inhibition and Analysis
- Proteoglycans and glycosaminoglycans research
- DNA and Nucleic Acid Chemistry
- Chemical Synthesis and Analysis
- Drug Solubulity and Delivery Systems
- 3D Printing in Biomedical Research
- MXene and MAX Phase Materials
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- Microfluidic and Bio-sensing Technologies
- Electrostatics and Colloid Interactions
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Polymer Surface Interaction Studies
Centre National de la Recherche Scientifique
2013-2024
Université Paris-Saclay
2006-2024
Institut Galien Paris-Saclay
2013-2023
Université Paris Cité
2022
Université Paris-Sud
2009-2019
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2009-2019
Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique
2012-2018
Inserm
2011
Harvard University
2009
Institut Gustave Roussy
2008
Nucleoside analogues display significant anticancer or antiviral activity by interfering with DNA synthesis. However, there are some serious restrictions to their use, including rapid metabolism and the induction of resistance. We have discovered that linkage nucleoside squalene leads amphiphilic molecules self-organize in water as nanoassemblies 100−300 nm, irrespective analogue used. The squalenoyl gemcitabine exhibited superior vitro human cancer cells gemcitabine-resistant murine...
Background: Because of the described hazards related to inhalation manufactured nanoparticles, we investigated lung toxicity biodegradable poly (lactide-co-glycolide) (PLGA) nanoparticles displaying various surface properties on human bronchial Calu-3 cells. Methods: Positively and negatively charged as well neutral were tailored by coating their with chitosan, Poloxamer, or (vinyl alcohol), respectively. Nanoparticles characterized in terms size, zeta potential, chemical composition,...
CD44 receptor protein is found to be overexpressed by many tumors and identified as one of the most common cancer stem cell surface markers including affecting colon, breast, pancreas, head neck, making this an attractive for therapeutic targeting. In study, 2'-F-pyrimidine-containing RNA aptamer (Apt1), previously selected against CD44, was successfully conjugated PEGylated liposomes using thiol-maleimide click reaction. The conjugation Apt1 confirmed change in size zeta potential migration...
Despite the wide interest raised by lung administration of nanoparticles (NPs) for treatment various diseases, little information is available on their effect toward airway epithelial barrier function. In this study, potential damage pulmonary epithelium upon exposure to poly(lactide-co-glycolide) (PLGA) NPs has been assessed in vitro using a Calu-3-based model bronchial barrier. Positively and negatively charged as well neutral PLGA were obtained coating surface with chitosan (CS),...
Cancer stem cells (CSCs) are a subset of tumor that has the ability to self-renew and generate diverse comprise mass. The cell-surface glycoprotein CD44 is one most common surface markers used identify CSCs. Aptamers synthetic oligonucleotides selected from pools random sequences can bind wide range targets with high affinity specificity. In this study, systematic evolution ligands by exponential enrichment (SELEX) technology was isolate RNA aptamers using human recombinant full-length...
To design nanoparticle (NP)-based drug delivery systems for pulmonary administration, biodegradable materials are considered safe, but their potential toxicity is poorly explored. We here explore the lung in mice of nanoparticles (NPs) and compare it to non-biodegradable ones. NP formulations poly(d,l-lactide-co-glycolide) (PLGA) coated with chitosan (CS), poloxamer 188 (PF68) or poly(vinyl alcohol) (PVA), which renders 200 nm NPs positive, negative neutral surface charge respectively, were...
Abstract This study describes a new simple method to obtain high loading of anticancer or antiviral nucleoside analogues into “stealth” poly(ethylene glycol) (PEG)‐coated nanoassemblies. These nanodevices are obtained by co‐nanoprecipitation in water (i) squalenoyl prodrugs the bioconjugation natural lipid squalene with either drug gemcitabine (Gem‐Sq) deoxycytidine (ddC‐Sq) (ii) PEG derivative cholesterol (Chol‐PEG) (Sq‐PEG). It was found that both derivatives (Chol‐PEG Sq‐PEG) were...
To introduce optical imaging among methods available to follow nanoparticle biodistribution, we have evaluated the concept of covalently labeling poly(lactide-co-glycolide) (PLGA) with a near-infrared (NIR) dye obtain stable NIR fluorescent nanoparticles. PLGA was coupled (DY-700, Dyomics) by an amide bond 38% efficiency. Incorporating 1% this conjugate into nanoparticles stabilised polyvinyl alcohol (PVA) leads (NPs) without affecting their colloidal characteristics (average diameter,...
The dynamics of the formation siRNA-lipoplexes coated with hyaluronic acid (HA) and parameters influencing their supramolecular organization were studied. insertion a HA-dioleylphosphatidylethanolamine (DOPE) conjugate in liposome structure as well subsequent complexation siRNA increased size. Lipoplexes around 110 nm at high ± charge ratios zeta potential +50 mV 230 low ratios, that decreased to negative values, reaching −45 mV. addition did not compromise binding liposomes, although these...
Modified nanoparticles (NPs) can interact with the immune system by causing its activation to fight tumors or for vaccination. During this activation, dendritic cells (DCs) are effective in generating robust response. However, effect of nanomaterials on cell (DC) maturation, and associated adjuvant effect, should be assessed as a novel biocompatibility criteria biomaterials since consequences may constitute potential complications nanomedicine. Among emerging biomaterials,...
Oral lichen planus (OLP) is an ongoing and chronic inflammatory disease affecting the mucous membrane of oral cavity. Currently, treatment choice consists in direct application into buccal cavity semisolid formulations containing a corticosteroid molecule to decrease signs symptoms. However, this administration route has shown various disadvantages limiting its clinical use efficacy. Indeed, frequency incorrect preparation may lead poor efficacy limit compliance. Furthermore, saliva...