Denise Treue
A5002077225- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Advanced Breast Cancer Therapies
- Lung Cancer Treatments and Mutations
- Breast Cancer Treatment Studies
- Bioinformatics and Genomic Networks
- BRCA gene mutations in cancer
- RNA Research and Splicing
- Cancer Cells and Metastasis
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- COVID-19 Clinical Research Studies
- Esophageal Cancer Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Colorectal Cancer Treatments and Studies
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Long-Term Effects of COVID-19
- Cancer, Stress, Anesthesia, and Immune Response
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Renal cell carcinoma treatment
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
Charité - Universitätsmedizin Berlin
2015-2025
Humboldt-Universität zu Berlin
2017-2024
Freie Universität Berlin
2017-2024
Heidelberg University
2014-2019
University Hospital Heidelberg
2014-2019
Klinik für Frauenheilkunde
2019
Sana Klinikum
2019
Universitätsklinikum Erlangen
2019
Rotkreuzklinikum München
2019
Helios Hospital Berlin-Buch
2019
A DNA methylation–based classifier facilitates the differentiation of primary lung and metastatic head neck cancer with diagnostic accuracy.
Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha, PIK3CA, is one of the most frequently mutated genes in breast cancer, and mutation status PIK3CA has clinical relevance related to response therapy. The aim our study was investigate gene evaluate concordance between NGS SGS for important hotspot regions exon 9 20, additional hotspots outside these exons using NGS, correlate with clinicopathological characteristics cohort.In current study, next-generation sequencing...
Abstract Objective to assess reactogenicity and immunogenicity of heterologous prime-boost immunisations ChAdOx1-nCoV19 (Vaxzevria, ChAdOx) followed by BNT162b2 (Comirnaty, BNT) compared homologous BNT/BNT immunisation. Design prospective, observational cohort study. Setting unicenter study in a health care workers at tertiary center Berlin, Germany. Participants 340 immunised between 27 December 2020 21 May 2021 Charité - Universitätsmedizin Germany Main outcome measures the main outcomes...
Abstract Background Increasing evidence suggests that secondary sclerosing cholangitis (SSC), which can lead to cirrhosis or liver failure, may be a hepatobiliary long-term complication of COVID-19. The aim this study was estimate the frequency and outcome COVID-19 sequela identify possible risk factors. Methods This observational study, conducted at University Hospital Charité Berlin Unfallkrankenhaus Berlin, Germany, involved hospitalized patients with pneumonia, including 1082 ventilated...
Cancer medicine relies on the paradigm that cancer is an organ‐ and tissue‐specific disease, which basis for classifying tumors. With extensive genomic information now available tumors it possible to conduct analyses reveal common genetic features across types explore whether established anatomy‐based tumor classification actually reflected level, might provide important guides new therapeutic directions. Here, we have conducted analysis of similarity from 14 major entities using somatic...
Purpose: Platinum-based drugs, in particular cisplatin (cis-diamminedichloridoplatinum(II), CDDP), are used for treatment of squamous cell carcinoma the head and neck (SCCHN). Despite initial responses, CDDP often results chemoresistance, leading to therapeutic failure. The role primary resistance at subclonal level treatment-induced clonal selection development remains unknown.Experimental Design: By applying targeted next-generation sequencing, fluorescence situ hybridization,...
Abstract Purpose: We evaluated mRNA signatures to predict response neoadjuvant PD-L1 inhibition in combination with chemotherapy early triple-negative breast cancer. Experimental Design: Targeted sequencing of 2,559 transcripts was performed formalin-fixed, paraffin-embedded samples from 162 patients the GeparNuevo trial. focused on validation four predefined gene and differential expression analyses for new predictive markers. Results: Two [GeparSixto signature (G6-Sig) IFN (IFN-Sig)] were...
Next-generation sequencing (NGS) can be used for comprehensive investigation of molecular events in breast cancer. We evaluated the relevance genomic alterations response to neoadjuvant chemotherapy (NACT) GeparSepto trial.Eight hundred fifty-one pretherapeutic formalin-fixed paraffin-embedded (FFPE) core biopsies from study were sequenced. The panel included 16 genes mutational (AKT1, BRAF, CDH1, EGFR, ERBB2, ESR1, FBXW7, FGFR2, HRAS, KRAS, NRAS, SF3B1, TP53, HNF1A, PIK3CA, and PTEN) 8...
We performed next-generation sequencing (NGS) in the CONKO-001 phase III trial to identify clinically relevant prognostic and predictive mutations conducted a functional validation The Cancer Genome Atlas (TCGA) data.Patients of received curatively intended surgery for pancreatic adenocarcinoma (PDAC) followed by adjuvant chemotherapy with gemcitabine (Gem) or observation only (Obs). Tissue samples 101 patients were evaluated NGS 37 genes. Cox proportional hazard models applied survival...
The PI3K signaling pathway is frequently dysregulated in breast cancer, and mutations PIK3CA are relevant for therapy resistance HER2-positive (HER2pos) cancer. Mutations exons 9 or 20 may have different impacts on response to neoadjuvant chemotherapy-based treatment regimens.
<title>Abstract</title> Lung cancer, the leading cause of cancer-related mortality, presents major challenges for both standard-of-care therapies and innovative treatments like CAR T cells due to tumor heterogeneity resistance. To aid in personalized treatment decisions, preclinical models that incorporate these patient-specific factors are essential. In this study, we developed a platform using matched lung tumoroids healthy organoids. The retained identity original tumors, as confirmed by...
Extrachromosomal DNA (ecDNA) is a common source of oncogene amplification across many types cancer. The non-Mendelian inheritance ecDNA contributes to heterogeneous tumour genomes that rapidly evolve resist treatment. Here, using single-cell and live-cell imaging, single-micronucleus sequencing, computational modelling, we demonstrate elevated levels predisposes cells micronucleation. Damage on ecDNA, commonly arising from replication stress, detaches the chromosomes upon which they...
// Jan Budczies 1,2 , Nicole Pfarr 2,3,4 Albrecht Stenzinger 2,3,5,6 Denise Treue 1 Volker Endris 2,3 Fakher Ismaeel 7 Nikola Bangemann 8 Jens-Uwe Blohmer Manfred Dietel Sibylle Loibl 9 Frederick Klauschen Wilko Weichert 2,3,4,5 and Carsten Denkert Institute of Pathology, Charité University Hospital, Berlin, Germany 2 German Cancer Consortium (DKTK), partner sites Heidelberg Munich, 3 Heidelberg, 4 Technical Munich (TUM), 5 National Center for Tumor Diseases (NCT), 6 Department Integrated...
The human Y-box binding protein 1 (YB-1) is known to be a promising target for cancer therapy. We have demonstrated that YB-1 plays an important role in the adenoviral life cycle by regulating E2-gene expression. Thus, we studied oncolytic effect of recombinant adenovirus Ad-Delo3-RGD, which transactivation domain CR3 E1A ablated enable viral replication only positive cells. In vitro Southern Blot analysis and cytopathic assays demonstrate high anti-glioma potency, was significantly...
Recent advances in cancer research largely rely on new developments microscopic or molecular profiling techniques offering high level of detail with respect to either spatial features, but usually not both. Here, we present a novel machine learning-based computational approach that allows for the identification morphological tissue features and prediction properties from breast imaging data. This integration microanatomic information tumors complex data, including protein gene expression,...
Abstract Background Pancreatic cancer remains a fatal disease. Experimental systems are needed for personalized treatment strategies, drug testing and to further understand tumor biology. Cell cultures can serve as an excellent preclinical platform, but their generation challenging. Methods Tumor cells from surgically removed pancreatic ductal adenocarcinoma (PDAC) specimens were cultured under novel protocols. Cellular growth composition analyzed culture conditions continuously optimized....
Abstract Perivascular epithelioid cell tumors (PEComas) form a family of rare mesenchymal neoplasms that typically display myomelanocytic differentiation. Upregulation mTOR signaling due the inactivation TSC1/2 (Tuberous Sclerosis 1 and 2) is believed to be key oncogenic driver in this disease . Recently, subgroup PEComas harboring TFE3 ( Transcription Factor E3 ) rearrangements presenting with distinctive morphology has been identified. aberrations are deemed mutually exclusive PEComa, two...
Dexamethasone has become the standard of care for severe coronavirus disease 2019 (COVID-19), but its virological impact is poorly understood. The objectives this work were to characterize kinetics acute respiratory syndrome 2 (SARS-CoV-2) concentration in upper tract (URT) and antibody response patients with (D+) without (D-) dexamethasone treatment.Data biosamples from hospitalized COVID-19, enrolled between 4th March 11th December 2020 a prospective observational study, analysed....
Abstract Acute myeloid leukemia (AML) is a heterogeneous disease characterized by genomic aberrations in oncogenes, cytogenetic abnormalities, and an aberrant epigenetic landscape. Nearly 50% of AML cases will relapse with current treatment. A major source therapy resistance the interaction mesenchymal stroma leukemic cells resulting therapeutic protection. We aimed to determine pro-survival/anti-apoptotic protein networks involved protection cells. Proteomic profiling cultured primary ( n =...
Objectives: Severe pneumonia may evoke acute lung injury, and sphingosine-1-phosphate is involved in the regulation of vascular permeability immune responses. However, role producing sphingosine kinase 1 remains elusive. We examined system regulating pulmonary barrier function bacterial pneumonia. Design: Controlled, vitro, ex vivo, vivo laboratory study. Subjects: Female wild-type SphK1 -deficient mice, 8–10 weeks old. Human postmortem tissue, human blood–derived macrophages, microvascular...
The maturation status of dendritic cells determines whether interacting T are activated or if they become tolerant. Previously we could induce cell tolerance by applying a 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitor (HMGCRI) atorvastatin, which also modulates MHC class II expression and has therapeutic potential in autoimmune disease. Here, aimed at elucidating the impact this strategy on differentiation as consequence alterations function. We investigated effect HMGCRI...
Immune checkpoint inhibition leads to response in some patients with head and neck squamous cell carcinoma (HNSCC). Robust biomarkers are lacking date. We analyzed viral status, gene expression signatures, mutational load signatures whole exome RNA-sequencing data of the HNSCC TCGA dataset (n = 496) a validation set (DKTK MASTER cohort, n 10). Public single-cell from 17 HPV-negative were separately reanalyzed. APOBEC3-associated TCW motif mutations but not total single nucleotide variant...