A5004341867- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
- Biosimilars and Bioanalytical Methods
- Animal Disease Management and Epidemiology
- Protein purification and stability
- Synthesis and Characterization of Heterocyclic Compounds
- Antibiotics Pharmacokinetics and Efficacy
- Advanced Chemical Sensor Technologies
- Milk Quality and Mastitis in Dairy Cows
- Microbial infections and disease research
- Silicon Nanostructures and Photoluminescence
- Effects and risks of endocrine disrupting chemicals
- Viral gastroenteritis research and epidemiology
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Pharmacological Effects and Assays
- T-cell and Retrovirus Studies
- Pesticide Residue Analysis and Safety
- Pesticide and Herbicide Environmental Studies
- Microfluidic and Bio-sensing Technologies
- Virus-based gene therapy research
- Flow Measurement and Analysis
Lomonosov Moscow State University
2015-2025
Moscow State University
2014-2025
Vavilov Institute of General Genetics
2024-2025
National University of Science and Technology
2015-2019
Faculty (United Kingdom)
2016
Queen's University Belfast
2002-2007
Lateral flow immunoassay (LFIA) is a widely used express method and offers advantages such as short analysis time, simplicity of testing result evaluation. However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications. In this study, spherical nanogold labels along with new types nanopopcorns nanostars were prepared, characterized, applied for model protein antigen procalcitonin. It was found that label structure close to provided more...
Bilirubin (BR) is a product of hemoglobin breakdown, and its increasing levels in the blood may indicate liver disorders lead to jaundice. Kernicterus most dangerous newborns when unconjugated BR concentration can quickly rise toxic levels, causing neurological damage even death. The development an accurate, fast, sensitive sensor for detection will help reduce diagnostic time ensure successful treatment. In this study, we propose new method creating surface-enhanced Raman scattering...
New viral infections, due to their rapid spread, lack of effective antiviral drugs and vaccines, kill millions people every year. The global pandemic SARS-CoV-2 in 2019-2021 has shown that new strains viruses can widespread very quickly, causing disease death, with significant socio-economic consequences. Therefore, the search for methods combating different pathogenic is an urgent task, strategies based on nanoparticles are interest. This work demonstrates adsorption (virucidal) efficacy...
Whole blood, plasma, whole milk and urine samples from domestic goats (Capra hircus) in both liquid dried form were analysed by quantitative ELISA. Dried matrix prepared using two types of absorbance support: glass fibre strip (strip-dried samples) cellulose membrane (dried spots). Strip-dried have advantages over spots due to better cortisol recovery, reproducibility, ease sample aliquoting no need for recalculations against standard protocol. In the ELISA, best concordance between results...
Abstract An indirect competitive ELISA for the detection of chloramphenicol (CAP) in food animal origin (milk, meat, eggs) is described. Influence immunoreagent structure and composition on assay sensitivity specificity was investigated. Two CAP derivatives were used conjugation with proteins: succinate a diazo derivative CAP. Molar incorporation into coating conjugates also varied. To eliminate matrix effect results, special casein-containing buffer milk samples, whereas meat egg samples...
We report new sensing element based on double-etched porous silicon (DEPSi) for sensitive detection of influenza viruses (H1N1). The proposed structure provided efficient penetration virions into layer and trapping them. Adsorption the led to significant shift resonant frequency DEPSi coupled with a coil, measured by impedance spectrometer. limit was lower than 100 TCID50. results can be used invention H1N1 sensor, which provide rapid, label-free low-cost influenza.
In this study, new types of nanogold labels for lateral flow immunoassay model antigen procalcitonin based on photometric and surface-enhanced Raman scattering methods detection were obtained. The linear range determination applying gold nanoflowers as a label was between 0.5 10 ng/mL. limit achieved at 0.1 ng/mL that five times lower than the sensitivity traditional with spherical nanoparticles label. addition, conjugate polyclonal antibodies against labeled 4-mercaptobenzoic acid reporter...
Nanomaterials are a central pillar in modern medicine. They thought to optimize drug delivery, enhance therapeutic efficacy, and reduce side-effects. To foster this technology, analytical methods needed validate not only the localization distribution of these nanomaterials, but also their compatibility with cells, drugs, release. In present work, we assessed nanoparticles based on porous silicon (pSiNPs) loaded clinically used tyrosine kinase inhibitor sunitinib for effectiveness release,...
The sensitive BRET system for the homogeneous immunoassay of a low-molecular weight antigen was developed using progesterone as an example. Two thermostable mutants Luciola mingrelica firefly luciferase (Luc)-the "red" mutant with λmax.em = 590 nm (RedLuc) and "green" 550 (GreenLuc)-were tested donors. water-soluble Alexa Fluor 610× (AF) dye selected acceptor because its two absorption maxima, located at 610 nm, are close to bioluminescence maxima GreenLuc RedLuc, respectively. methods...