A5014780359- Glioma Diagnosis and Treatment
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Nanoplatforms for cancer theranostics
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Radiomics and Machine Learning in Medical Imaging
- Photoacoustic and Ultrasonic Imaging
- Meningioma and schwannoma management
- Brain Tumor Detection and Classification
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Research and Splicing
- Neurosurgical Procedures and Complications
- Mitochondrial Function and Pathology
- Vascular Malformations Diagnosis and Treatment
- RNA modifications and cancer
- Global Health and Surgery
- Teratomas and Epidermoid Cysts
- Circular RNAs in diseases
- Trigeminal Neuralgia and Treatments
- Global Health Workforce Issues
- Testicular diseases and treatments
- Cerebrospinal fluid and hydrocephalus
- Photodynamic Therapy Research Studies
- Ferroptosis and cancer prognosis
University of Minnesota Medical Center
2024
Massachusetts General Hospital
2019-2023
Harvard University
2019-2023
University of Minnesota
2023
Boston University
2023
Yale University
2022-2023
Yale New Haven Hospital
2022
Health First
2019
Guntur Medical College
2015
Liquid biopsy offers a minimally invasive tool to diagnose and monitor the heterogeneous molecular landscape of tumors over time therapy. Detection TERT promoter mutations (C228T, C250T) in cfDNA has been successful for some systemic cancers but yet be demonstrated gliomas, despite high prevalence these glioma tissue (>60% all tumors).Here, we developed novel digital droplet PCR (ddPCR) assay that incorporates features improve sensitivity allows simultaneous detection longitudinal monitoring...
Tumour cells release diverse populations of extracellular vesicles (EVs) ranging in size, molecular cargo, and function. We sought to characterize mRNA protein content EV subpopulations released by human glioblastoma (GBM) expressing a mutant form epidermal growth factor receptor (U87
Biopsies of tumors located in deep midline structures require highly accurate stereotaxy to safely obtain lesional tissue suitable for molecular and histological analysis. Versatile platforms are needed meet a broad range technical requirements surgeon preferences. The authors present their institutional experience with the robotic stereotactic assistance (ROSA) system series robot-assisted biopsies pediatric brainstem thalamic tumors.
BackgroundMalignant gliomas are rapidly progressive brain tumors with high mortality. Fluorescence guided surgery (FGS) 5-aminolevulinic acid (5-ALA) provides fluorescent delineation of malignant tissue, which helps achieve maximum safe resection. 5-ALA-based fluorescence is due to preferential accumulation the fluorophore protoporphyrin-IX (PpIX) in glioma tissue. Additionally, cells release extracellular vesicles (EVs) carry biomarkers disease. Herein, we performed animal and human studies...
H3K27M-mutant diffuse midline glioma is a recently classified unique entity predominantly affecting pediatric patients and rarely adults. The clinicopathologic features in adults remain poorly characterized. A 36-year-old man presented with subacute progressive cognitive visual deterioration, hydrocephalus requiring ventricular shunting. MRI revealed diffusely infiltrating lesion gliomatosis cerebri growth pattern, multiple foci of contrast enhancement, leptomeningeal involvement....
Over the last 20 years, gliomas have made up over 89% of malignant CNS tumor cases in American population (NIH SEER). Within this, glioblastoma is most common subtype, comprising 57% all glioma cases. Highly aggressive, this deadly disease known for its high genetic and phenotypic heterogeneity, rendering a complicated course. The current standard-of-care consists maximally safe resection concurrent with chemoradiotherapy. However, despite advances technology therapeutic modalities, rates...
Sequencing studies have provided novel insights into the heterogeneous molecular landscape of glioblastoma (GBM), unveiling a subset patients with gene fusions. Tissue biopsy is highly invasive, limited by sampling frequency and incompletely representative intra-tumor heterogeneity. Extracellular vesicle-based liquid provides minimally invasive alternative to diagnose monitor tumor-specific aberrations in patient biofluids. Here, we used targeted RNA sequencing screen GBM tissue matched...
Background: In glioblastoma (GB), tissue is required for accurate diagnosis and subtyping. Tissue can be obtained through resection or (stereotactic) biopsy, but these invasive procedures provide risks patients. Extracellular vesicles (EVs) are small, cell-derived that contain miRNAs, proteins, lipids, possible candidates liquid biopsies. GB-derived EVs found in the blood of patients, it difficult to distinguish them from circulating non-tumor EVs. 5-aminolevulinic acid (5-ALA) orally...
Liquid biopsy provides a minimally invasive platform for the detection of tumor-derived information, including hotspot mutations, such as BRAF V600E. In this study, we provide evidence technical development ddPCR assay V600E mutations in plasma patients with glioma or brain metastasis. small patient cohort (n = 9, n 5 V600E, 4 WT, healthy control), were able to detect mutation 4/5 V600E-tissue confirmed mutant tumors, and none WT tumors. We also two metastatic longitudinal monitoring, where...
Abstract Liquid biopsy is a minimally invasive alternative to surgical biopsy, encompassing different analytes including extracellular vesicles (EVs), circulating tumour cells (CTCs), DNA (ctDNA), proteins, and metabolites. EVs are released by virtually all cells, but at higher rate faster cycling, malignant cells. They encapsulate cargo native the originating cell can thus provide window into landscape. often analysed in bulk which hinders analysis of rare, tumour‐specific EV subpopulations...
OBJECTIVE Training of international medical graduates (IMGs) offers opportunities for the US neurosurgery community to engage global talent pool and impact national healthcare. Here, authors analyzed time trend IMGs matching into programs identified potential enhancing IMG engagement. METHODS The National Resident Matching Program (NRMP) match results, NRMP program director (PD) surveys, applicant surveys from 2013 2022. Regression methods were used analyze trends. RESULTS Between 2022,...
OBJECTIVE Gamma Knife radiosurgery (GKRS) is an established surgical option for the treatment of trigeminal neuralgia (TN), particularly high-risk candidates and those with recurrent pain. However, outcomes after three or more GKRS treatments have rarely been reported. Herein, authors reviewed among patients who had undergone procedures TN. METHODS The conducted a multicenter retrospective analysis at least TN between July 1997 April 2019 two different institutions. Clinical characteristics,...
External ventricular drains (EVDs or ventriculostomies) are frequently required for acute management of intracranial hypertension and monitoring pressure.Patients with ventriculostomies co-managed by multiple specialists including neurologists, neuro-intensivists, trauma specialists, emergency department physicians neurosurgeons.We provide background on the indications, placement technique, troubleshooting complications related to this procedure.We also discuss emerging best practices...
Rectal venous malformations (VMs) are rare clinical entities with variable patterns of presentation. Treatment requires unique, targeted strategies based on the symptoms, associated complications, and location, depth, extent lesion. We present a case large, isolated rectal VM treated by direct stick embolization (DSE) using transanal minimally invasive surgery (TAMIS). A 49-year-old man had presented mass incidentally detected computed tomography urography. Magnetic resonance imaging...